Comparison of anterior capsule electron microscopy findings with and without trypan blue stain

Trypan blue staining for capsulorhexis: Ultrastructural effect on lens epithelial cells and capsules

“…Although the lens epithelium was not clearly identified in the frozen sections, it could barely be discerned as a refractive layer and did not stain with trypan blue. 18 Rangaraj et al 19 evaluated trypan blue-stained anterior lens capsules from 2 human eyes. Light microscopy of toluidine bluestained sections was unremarkable, and the lens capsule appeared focally ragged and irregular on TEM.…”

Trypan blue dye for anterior segment surgeries

“…2,5,48,49 The effect of TB on lens epithelial cells has been tested using different techniques. 50,51 Although in vitro studies found no effect on the viability of LECs at concentrations ranging from 0.025 to 5.0 mg/ml, 52 Nanavaty et al 11 reported a significant decrease in LEC density covering the anterior lens capsule after the use of 0.0125% TB for 30 s compared with that of untreated eyes. Portes et al 53 found LEC death with 0.1% TB which supports that staining with TB can help reduce the incidence of posterior capsule opacification.…”

Capsular staining: recent developments

“…Rangaraj et al [43] compared the ultrastructure of the anterior capsule with and without Trypan Blue. While light microscopy was unremarkable, electron microscopy showed that the outer limit of the stained capsule was focally ragged and irregular, and devoid of the electronloosened bubbles present in the unstained section.…”