Purpose: To test whether some biometry measurements provided by the Lenstar LS900 compared well with the AL-Scan, Pentacam rotating Scheimpflug camera, Ultrasound Biomicroscopy (UBM) and Tomey EM-3000. Methods: 201 patients having routine cataract surgery had standard preoperative assessment. In this clinical study, the axis length (AL) and lens thickness (LT) were taken by Lenstar LS900 and AL-Scan; anterior chamber depth (ACD) were taken by Lenstar LS900, A-scan, Pentacam and UBM; central corneal thickness (CCT) were taken by Lenstar LS900, Pentacam and Tomey EM-3000. The results were compared using a Wilcoxon-Mann-Whitney U test and Pearson correlation calculations. Agreement was assessed through intraclass correlation coefficients and Bland-Altman plots.Results: The highest correlation was found between Lenstar and AL-Scan for AL (r = 0.975; P <0.001). For LT measurements, the correlation between these two devices was also good (r = 0.699; P <0.001). Excellent correlations were showed between Lenstar and pentacam or UBM for ACD (r=0.948, 0.704, respectively; both P <0.001), but AL-scan (r=0.453, P <0.001). According to CCT, correlation between Lenstar and Pentacam or Tomey EM-3000 were both excellent (r=0.817, 0.882, respectively; both P <0.001).Conclusions: In phakic eyes of cataract patients, measurements of AL, LT, ACD, and CCT from Lenstar LS900 yielded results that correlated very well with other clinical instruments.