Comparison of Antibiotic Resistance Profile and Biofilm Production of Staphylococcus aureus Isolates Derived from Human Specimens and Animal-Derived Samples

Abstract

Background: The diffusion of antimicrobial resistance is a significant concern for public health worldwide. Staphylococcus aureus represents a paradigm microorganism for antibiotic resistance in that resistant strains appear within a decade after the introduction of new antibiotics. Methods: Fourteen S. aureus isolates from human specimens and twenty-one from samples of animal origin, were compared for their antimicrobial resistance and biofilm capability. In addition, they were characterized at the molecular level to detect the antimicrobial resistance mecA gene and genes related with enterotoxin, toxin, and biofilm production. Results: Both phenotypic and molecular analysis showed main differences among human- and animal-derived isolates. Among the human-derived isolates, more multidrug-resistant isolates were detected and mecA gene, enterotoxin, and toxin genes were more prevalent. Different genes involved in biofilm production were detected with bap present only in animal-derived isolates and sasC present in both isolates, however, with a higher prevalence in the human-derived isolates. Biofilm capability was higher in human-derived isolates mainly associated to the sasC gene. Conclusions: The overall results indicate that human S. aureus isolates are more virulent and resistant than the isolates of animal origin randomly selected with no infection anamnesis. This study confirms that selection for more virulent and resistant S. aureus strains is related to the clinical practice.

Is Caretta Caretta a Carrier of Antibiotic Resistance in the Mediterranean Sea?

“…When the biochemical analysis was not exhaustive, amplification and sequencing of the 464 bp fragment of the 16S rDNA were carried out. An aliquot (2 µL) of the bacterial lysate, prepared as previously described [47], was used to amplify the 464 bp internal fragment of the 16S rDNA using One Taq DNA polymerase (NEB), using primer pairs and the corresponding annealing temperatures listed in Table 4. After confirmation through agarose (1% w/v) gel electrophoresis, the polymerase chain reaction (PCR) products were purified and sequenced at Macrogen Inc. (Seoul, Korea, sequencer).…”

Superior Antibacterial Activity of Integral Lemon Pectin Extracted via Hydrodynamic Cavitation

“…The ability to control bacterial growth and colonization is of extreme practical importance in several fields, including prevention of food contamination in the food chain, in healthcare and even in the protection of cultural heritage to reduce biodeterioration processes . In the last years, the emergence of multi‐drug resistant strains occurred in several animals and environments, including wild and uncontaminated ones .…”

“…In the last years, the emergence of multi‐drug resistant strains occurred in several animals and environments, including wild and uncontaminated ones . One of the most frequent antibiotic resistant strain causing food poisoning is the Gram positive Staphylococcus aureus …”

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“…This assumption is supported by the report of Wójcik et al, 2018 [ 35 ] that demonstrated the incorporation of perfluorinated compounds into model membranes resembling those typical to Gram-negative bacteria. Antibacterial activity was also tested against S. aureus ATCC 25923 and S. aureus ATCC 33862, two pathogenic, toxigenic and biofilm producer strains, frequently associated with both human and animal infections [ 2 ] and we found that both complexes were active in inhibiting the growth of both the strains with complex 1 more performing than complex 2 .…”

“…Bacterial infections in clinical and veterinary environments can frequently be due to different MDR bacterial strains, such as Staphylococcus aureus (S. aureus) and Escherichia coli ( E. coli ) , etc. [ 2 , 3 ]. In the frame of our study, we have previously synthesized, characterized and investigated the biological activity of Pt(II) complexes with 2-(5-perfluoropropyl)-1,2,4-oxadiazole-3yl)-pyridine ( pfpop ), 2-(5-perfluoroheptyl-1,2,4-oxadiazole-3yl)-pyridine ( pfhop ), 2-(3-perfluoropropyl-1-methyl-1,2,4-triazole-5yl)-pyridine ( pfptp ), and 2-(3-perfluoroheptyl-1-methyl-1,2,4-triazole-5yl)-pyridine ( pfhtp ) ligands, finding interesting data concerning both antitumor and antimicrobial activity [ 4 , 5 ].…”