Comparison of antibody responses in Atlantic cod (Gadus morhua L.) to Aeromonas salmonicida and Vibrio anguillarum

Viral and bacterial diseases of Atlantic cod Gadus morhua, their prophylaxis and treatment: a review

“…In this context, it is probably relevant that the Atlantic cod can mount a significant antibody response towards the LPS moiety of the Aeromonas salmonicida pathogen (supposed to be T cell independent), although immunisation with the LPS-containing Listonella anguillarum did not elicit similar antibody production (Lund et al 2006).…”

“…On the other hand, juveniles vaccinated at a weight of 2.2 g obtained good protection when challenged by bath 7 wk after vaccination (RPS = 77). The long-term protection after vaccination was not evaluated at this size, while cod vaccinated at 5 g were highly protected (RPS = 83) for as long as 28 wk after dip vaccination (Schrøder et al 2006). Vaccines administered by dip (immersion in a 1:10 diluted vaccine for 30 s) to 10 g cod also resulted in significantly better protection against classical vibriosis than bath vaccination (vaccine diluted 1:200 and the fish immersed for 30 min) (Schrøder et al 2006).…”

Vibriosis vaccines based on various sero-subgroups of Vibrio anguillarum O2 induce specific protection in Atlantic cod (Gadus morhua L.) juveniles

“…The specificity of the cod and rabbit antisera to V. anguillarum was determined by immunostaining of cell lysates on Western blot, as previously described [22]. The Bio-Rad Criterion electrophoresis and blotting system including pre-cast 12% Bis-Tris gels, premixed running buffer and XT MOPS sample buffer with reducing agent, were used.…”

Specificity and durability of antibody responses in Atlantic cod (Gadus morhua L.) immunised with Vibrio anguillarum O2b

“…The reason why only a few cod individuals reveal detectable antibody responses to V. anguillarum is unknown, but they appeared to reveal a highly specific and long lasting response. In the present study, we have compared the specificity of antibodies to V. anguillarum O2b induced in 2 cod individuals [6] with that in rabbit, using immunoblotting. Monoclonal antibodies (mAb) to V. anguillarum O2b was also included in the study.…”