Comparison of antiglobulin, direct, and anticomplement immunofluorescent staining for the identification of cytomegalovirus in cultured human fibroblasts

Abstract

The sequential development of intracellular antigens in cultured human embryonic fibroblasts infected with the AD-169 strain of cytomegalovirus was studied by means of antiglobulin, direct, and anticomplement immunofluorescent staining.Direct immunofluorescent staining, although less sensitive than the antiglobulin or anticomplement methods for the detection of early diffuse nuclear antigens, gave the clearest definition of the typical intracellular inclusions produced by cytomegalovirus.Quantitative complement fixation assays, carried out in parallel with anticomplement staining, showed that complement is fixed strongly by the intracellular antigens which develop in the late stages of infection.

Antisera to human cytomegalovirus produced in hamsters: reactivity in radioimmunoassay and other antibody assay systems

“…on September 12, 2020 by guest http://iai.asm.org/ Downloaded from gated with fluorescein isothiocyanate and used in direct FA staining with CMV-infected cells fixed at 100 h postinfection, the nuclear staining was more intense, and the cytoplasmic staining was less marked than that seen by indirect FA staining. These findings are in agreement with those obtained by Laing (16) using human antisera for direct and indirect FA staining of CMV-infected cells.…”

Serological Methods in the Identification and Characterization of Viruses

“…The anticomplement staining procedure employs unlabeled viral antibody (lgG) that will fix complement following its interaction with antigen. An anti-C3 fluorescent antibody conjugate is used to detect the presence of bound complexes (Laing, 1974). The sensitivity of this technique is superior to that of the direct staining procedure.…”