Comparison of antimicrobial activity of selected, commercially available wound dressing materials

Szweda, Piotr; Gorczyca, Grzegorz; Tylingo, Robert

doi:10.12968/jowc.2018.27.5.320

Cited by 22 publications

(13 citation statements)

References 37 publications

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“…By contrast, MH, made from the nectar of the manuka bush (Leptospermum scoparium), remains active after catalase treatment and is effective at low concentrations, and is therefore much more effective as an antimicrobial agent (Molan and Russell 2015). Manuka honey has proven effective at both the treatment and prevention of growth of multi-drug resistant bacteria (Jenkins et al 2011a), fungi (Irish et al 2006) and biofilms (Maddocks et al 2012); this has made it a suitable alternative to common antimicrobial treatments, especially in wound care (Guthrie et al 2014;Abd El-Malek et al 2017;Watson et al 2017;Hussain 2018;Szweda et al 2018). There are many factors contributing to the antimicrobial activity of MH, including low pH, high osmolarity and the presence of phytochemicals (Mandal and Mandal 2011).…”

Section: Introductionmentioning

confidence: 99%

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

et al. 2019

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Aim The aim of this study was to test the hypothesis that Manuka honey (MH) chelates iron and promotes an iron‐limiting environment, which contributes to its antimicrobial activity. Methods and Results Employing a ferrozine‐based assay, we observed that MH is an iron chelator that depletes iron from solution. Siderophores are small molecules that bind ferric iron (III) with high affinity and their levels are upregulated by bacteria under iron‐limiting conditions. We demonstrated by quantitating siderophore production that Escherichia coli and Pseudomonas aeruginosa treated with MH sub‐minimum inhibitory concentrations (sub‐MIC) experience an iron‐limiting environment and increase siderophore production. In addition, supplementation with ferrous iron (II) significantly increased growth of E. coli, Staphylococcus aureus and P. aeruginosa cultured at their MH MIC above that observed in nonsupplemented controls. By contrast, supplementation with ferric iron (III) significantly increased growth for only E. coli and P. aeruginosa, above their nonsupplemented controls. Conclusions Manuka honey chelates iron, thereby generating an iron‐limiting environment for E. coli and P. aeruginosa, and to a lesser extent S. aureus, which contributes to its antimicrobial properties. Significance and Impact of the Study Our work demonstrates that MH‐induced iron chelation is an antimicrobial mechanism that differentially impacts the bacterial species tested here. Iron chelation affects multiple diverse physiological processes in bacteria and would contribute to the lack of bacterial resistance to MH. Iron metabolism is tightly regulated; bacteria require this essential nutrient for survival, but in excess it is toxic. Additional exploration of MH’s iron chelation mechanism will facilitate its future use in mainstream medicine.

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Section: Introductionmentioning

confidence: 99%

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

et al. 2019

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“…However, both CMC and plasma-treated PCL possess high fluid absorption capacity. As a result, when the dressings absorb fluid and swell, bacteria available in the MH medium might be trapped inside their polymer matrix, leading to the reduction in bacteria colonies remained after being collected between 0 h and 1 h. On the other hand, even though PCLGelAg expressed a slower speed of kill against S. aureus , the dressing was able to reach maximal killing rate after 12 h of bacteria exposure and sustain the effect for at least 24 h. Indeed, the antimicrobial activity is further affected by the diverse silver species [ 42 ], which are in charge of the interaction with bacteria, and base materials, which manage the silver released duration and concentration [ 37 ]. Combining with the data from the agar diffusion assay and microdilution method, it is suggested that PCLGelAg should be used topically and changed daily to maintain the highest bactericidal performance.…”

Section: Discussionmentioning

confidence: 99%

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Thuan

Nguyen

et al. 2021

Polymers

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(1) Background: Wounds with damages to the subcutaneous are difficult to regenerate because of the tissue damages and complications such as bacterial infection. (2) Methods: In this study, we created burn wounds on pigs and investigated the efficacy of three biomaterials: polycaprolactone-gelatin-silver membrane (PCLGelAg) and two commercial burn dressings, Aquacel® Ag and UrgoTulTM silver sulfadiazine. In vitro long-term antibacterial property and in vivo wound healing performance were investigated. Agar diffusion assays were employed to evaluate bacterial inhibition at different time intervals. Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and time-kill assays were used to compare antibacterial strength among samples. Second-degree burn wounds in the pig model were designed to evaluate the efficiency of all dressings in supporting the wound healing process. (3) Results: The results showed that PCLGelAg membrane was the most effective in killing both Gram-positive and Gram-negative bacteria bacteria with the lowest MBC value. All three dressings (PCLGelAg, Aquacel, and UrgoTul) exhibited bactericidal effect during the first 24 h, supported wound healing as well as prevented infection and inflammation. (4) Conclusions: The results suggest that the PCLGelAg membrane is a practical solution for the treatment of severe burn injury and other infection-related skin complications.

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“…In "in vitro" studies from 2015, 2017, and 2018, it was shown that Mepilex® Ag was highly effective against some of the most common bacterial pathogens in burns, such as staphylococcus aureus and pseudomonas aeruginosa [115][116][117]. However, it has also shown to have a negative effect on cell survival [115,118].…”

Section: Cytotoxicity Of Mepilex®agmentioning

confidence: 99%

Studying the healing and long-term outcomes of two partial thickness wound models using different wound dressings

Karlsson

2019

Linköping University Medical Dissertations

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Background: Safe and effective wound dressing treatments are important for proper wound healing. Such procedures therefore need to be evidence-based regarding the most important outcome measures such as healing time, less discomfort for the patient, duration of hospital care and, importantly, less scarring. As the relation between longer healing times and more severe scarring is known, it is important to find dressing treatments that reduces such complications by providing fast and proper wound healing. In this thesis, four established wound dressing treatments (hydrofibre covered with film; porcine xenografts and polyurethane foam, with and without silver), were evaluated for two types of acute, partial thickness wounds: split thickness skin graft (STSG) donor sites and partial thickness burn wounds in two randomised, controlled clinical trials (RCT) with longterm scar follow ups. The relations between factors thought to influence wound healing and scarring as sex, infection, wound extent and depth, healing time and skin grafting were also investigated in these two wound models. Methods: Data from these trials were collected on sex, infection rates, wound depth and extent, need of skin grafting, healing times and scarring frequency together with demographic data. Scars were evaluated at 8 years in Study II and III and at 6 and 12 months after injury in Study V.

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Comparison of antimicrobial activity of selected, commercially available wound dressing materials

Abstract: Some important differences in the antimicrobial potential of investigated materials have been found. The presented results could be of interest to clinicians managing wounds.

Cited by 22 publications

References 37 publications

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

Manuka honey chelates iron and impacts iron regulation in key bacterial pathogens

The Efficacy of Silver-Based Electrospun Antimicrobial Dressing in Accelerating the Regeneration of Partial Thickness Burn Wounds Using a Porcine Model

Studying the healing and long-term outcomes of two partial thickness wound models using different wound dressings

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