Comparison of Antinociceptive Effects of Total, Water, Ethyl Acetate, Ether and n-butanol Extracts of Phlomis anisodonta Boiss. and Indomethacin in Mice

Hajarolasvadi, Naghmeh; Zamani, Mohammad Jafar; Sarkhail, Parisa; Khorasani, Reza; Mohajer, Masoomeh; Amin, Gholamreza; Shafiee, Abbas; Sharifzadeh, Mohammad; Abdollahi, Mohammad

doi:10.3923/ijp.2006.209.212

Cited by 5 publications

(3 citation statements)

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“…The difference between inflamed and right knee , s joint width at 6 time points was calculated (indicating the degree of inflammation) and was compared to the amount for vehicle group (N) [26]. …”

Section: Methodsmentioning

confidence: 99%

Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats

et al. 2016

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BackgroundMany plants have been introduced in Iranian traditional medicine for treatment of different joint problems including knee pain. Topical application of the mixture of Lawsonia inermis L. leaves (Henna) with aqueous extract of Ricinus communis L. leaves have been mentioned to have significant effects on reducing knee pain. The present study was designed to evaluate the analgesic and anti-inflammatory effects of the mixture of these two herbs in male rats.MethodsWe induced knee osteoarthritis as a model of chronic pain by intra-articular injection of mono sodium iodoacetate (MIA). Mechanical allodynia, hotplate latency test, spontaneous movements and gait analysis were used for the evaluation of analgesic activity. Anti-inflammatory activity was evaluated by measuring the diameter and the volume of the injected paw compared to contralateral paw. These tests were monitored at days 1, 3, 7, 14 and 21 of MIA administration. Histopathological evaluations were also used to assess the efficacy of the treatment on inflammation and lesions in knee tissue. In all tests, diclofenac topical gel was used as a positive control. The herbal extracts, their mixture, and vehicle or diclofenac gel were administered daily for 14 days by topical route.ResultsThe mixture of these two extracts significantly reduced the knee joint width and volume of the injected paws and also improved foot prints in gait analysis after 3 days of MIA injection. Analysis of mechanical allodynia (after 21 days), hotplate latency test (after 10 days), spontaneous movements (after 7 days) and in positive control group (after 3 days in all tests and in mechanical allodynia after 14 days) compared to the vehicle group, showed significant effects. Topical usage of the selected formulation made significant histopathological changes on the knee of the rats. Compared to the vehicle group, the tests and diclofenac groups showed less reactions characterized by negligible edema and a few scattered inflammatory lymphoid cells.ConclusionThe present findings showed that the present formulation not only was able to mitigate pain and inflammation in the paws but also made significant histopathological changes on the knee of the rats. Further studies are necessary to confirm the effect of the formulation.

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Section: Methodsmentioning

confidence: 99%

Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats

et al. 2016

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“…Phlomis species contain a diverse range of compounds, including iridoid glucosides [14][15][16], phenylpropanoid glycosides [17], flavonoid glycosides [18], lignans [19], neolignan glycosides [20], monoterpene glycosides [21], megastigmanes [22], diterpene glycosyl esters [23,24], nortriterpenes [25,26] and essential oils [5], which were reported to be responsible for various biological and pharmacological properties. Among them are the antinociceptive, anti-inflammatory [27][28][29], antioxidant [30][31][32][33][34], antimicrobial [35,36], wound-healing, antidiabetic [37,38] and antihemoroidal activity [39], as well as the antiplasmodial and anticancer activities [12,38,[40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

Chemical Constituents and Anticancer Activities of the Extracts from Phlomis × commixta Rech. f. (P. cretica × P. lanata)

Stefanakis,

Tsiftsoglou,

Mašković

et al. 2024

IJMS

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The present work is the first report on the ingredients of the P. × commixta hybrid, a plant of the genus Phlomis. So far, thirty substances have been isolated by various chromatographic techniques and identified by spectroscopic methods, such as UV/Vis, NMR, GC-MS and LC-MS. The compounds are classified as flavonoids: naringenin, eriodyctiol, eriodyctiol-7-O-β-D-glucoside, luteolin, luteolin-7-O-β-D-glucoside, apigenin, apigenin-7-O-β-D-glucoside, diosmetin-7-O-β-D-glucoside, quercetin, hesperetin and quercetin-3-O-β-D-glucoside; phenylpropanoids: martynoside, verbascoside, forsythoside B, echinacoside and allysonoside; chromene: 5,7-dihydroxychromone; phenolic acids: caffeic acid, p-hydroxybenzoic acid, chlorogenic acid, chlorogenic acid methyl ester, gallic acid, p-coumaric acid and vanillic acid; aliphatic hydrocarbon: docos-1-ene; steroids: brassicasterol and stigmasterol; a glucoside of allylic alcohol, 3-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranosyl-oct-1-ene-3-ol, was fully characterized as a natural product for the first time. Two tyrosol esters were also isolated: tyrosol lignocerate and tyrosol methyl ether palmitate, the latter one being isolated as a natural product for the first time. Moreover, the biological activities of the extracts from the different polarities of the roots, leaves and flowers were estimated for their cytotoxic potency. All root extracts tested showed a high cytotoxic activity against the Hep2c and RD cell lines.

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“…A number of Phlomis species (Lamiaceae) have been used in folk medicine as stimulants, anticough agents, and to treat gastric, intestinal and abdominal pains, as a tonic, sedative, carminative and astringent. [ 11 ] Various activities such as antinociceptive,[ 12 13 ] antigenotoxic and antioxidant,[ 14 ] antiulcerogenic, anticancer, anti-inflammatory and antiallergic activities have been reported for some Phlomis species. [ 11 ] This genus is rich in terpenoids, iridoids, flavonoids and other phenolic compounds that contribute potential biological effects like antioxidant activities to many of them.…”

Section: Introductionmentioning

confidence: 99%

Effect of Phlomis persica on glucose levels and hepatic enzymatic antioxidants in streptozotocin-induced diabetic rats

et al. 2010

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Methanol extract of the aerial parts of Phlomis persica Boiss. (Lamiaceae) (PPE) was studied to evaluate the effects of antidiabetic potential, by measuring fasting blood glucose, insulin, total antioxidant power (TAP), using ferric reducing antioxidant power (FRAP), lipid peroxidation (using thiobarbituric acid reactive substances, TBARS), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) on streptozotocin-induced diabetes in rats. Male Wistar rats were randomly divided into five groups of six animals each. Oral administration of PPE at doses of 100 and 200 mg/kg once a day for 10 days resulted in a significant reduction in fasting blood glucose and an increase in serum insulin levels, in comparison with diabetic control group. It also prevented diabetes-induced loss in body weight. Hepatic TAP increased and TBARS decreased following PPE treatments. The extract at 100 and 200 mg/kg increased the activity of hepatic SOD, CAT, and GPx in diabetic rats. It is concluded that PPE has antidiabetic potential that is comparable with glibenclamide. In conclusion, the results of the present study show positive effects of P. persica on experimental diabetes and thus the antidiabetic effect of PPE is related to its potential to inhibit hepatocellular oxidative stress.

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Comparison of Antinociceptive Effects of Total, Water, Ethyl Acetate, Ether and n-butanol Extracts of Phlomis anisodonta Boiss. and Indomethacin in Mice

Cited by 5 publications

References 16 publications

Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats

Preliminary investigation of the effects of topical mixture of Lawsonia inermis L. and Ricinus communis L. leaves extract in treatment of osteoarthritis using MIA model in rats

Chemical Constituents and Anticancer Activities of the Extracts from Phlomis × commixta Rech. f. (P. cretica × P. lanata)

Effect of Phlomis persica on glucose levels and hepatic enzymatic antioxidants in streptozotocin-induced diabetic rats

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