Comparison of Antioxidant Activity and Main Active Compounds Among Different Parts of Alpinia officinarum Hance Using High-Performance Thin Layer Chromatography-Bioautography

Abstract

Background: Alpinia officinarum Hance (ginger family) is an important Chinese medicine, especially in Southern China. Objective: A simple and effective high-performance thin-layer chromatography coupled with 2, 2-diphenyl-1-picrylhydrazyl bioautography (HPTLC-DPPH) and electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI-Q-TOF-MS/MS) method was developed for the bioactivity-based quality control of A. officinarum. Methods: The HPTLC-DPPH and ESI-Q-TOF-MS/MS were applied for the analysis of different parts of A. officinarum after using methanol extraction for 23 batches of taproot, four batches of aerial, and three batches of fibril parts. Results: The systematic evaluation showed that similar components in taproot and aerial parts make the major antioxidant activity. However, based on our evaluation, the antioxidant ability of the aerial parts is lower than the taproot parts. There is also a significant difference (P < 0.05) between taproot and fibril parts of the root. The chemical structures of compounds with the antioxidant capacity were tentatively identified as 5R-hydroxy-7-(4-hydroxy-3-methoxyphenyl)-1-phenyl-3-heptanone (band 1), kaempferide (band 2), and galangin (band 3) based on ESI-Q-TOF-MS/MS analytical results and further confirmed by standards. Conclusions: This identification indicated that two flavonoid compounds and one diarylheptanoid compound possessed high potentials to be used as the antioxidant biomarkers for the quality control of A. officinarum. Highlights: The comparison of different parts could be considered as guidelines for the usage of A. officinarum.

LC-MS/MS Screening of Phenolic Compounds in Wild and Cultivated Grapes Vitis amurensis Rupr.

“…The anthocyanins identified in V. amurensis in this study were previously identified and annotated in the vines [17] Solanium nigrum [18], Gaultheria Antarctica [19], and Vitis vinifera [20] and wheat [21]. Our identification of flavonoid compounds agrees with bibliographic data for Echinops [22], Rhodiola rosea [23], Ocimum [24], Alpinia officinarum [25], Brazilian propolis [26], Vitis vinifera [20], Rubus occidentalis [27], C. edulis [28], and Vaccinium macrocarpon [29].…”

A Concise Review of Current In Vitro Chemical and Cell-Based Antioxidant Assay Methods

“…Thus, it is imperative to develop new, reliable, simple, more sensitive, highly specific, and selective antioxidant assays with clearly identifiable reaction mechanisms and fully tested reaction conditions [ 17 , 23 ]. Examples of efforts in this regard include the use of malondialdehyde/high-performance liquid chromatography (MA/HPLC) assay and malondialdehyde/gas chromatography (MA/GC) assay for measuring lipid peroxidation, using high-performance thin-layer chromatography coupled with DPPH bioautography (HPTLC-DPPH) for quantifying antioxidant activity in the medicinal plant Alpinia officinarum, and combining EPR spectroscopy with a free radical scavenging method such as DPPH for quantifying the various antioxidants in natural extracts such as pomegranate fruit juice [ 23 , 88 , 89 ].…”

Research of 5 extracts of wild Amur grape Vitis amurensis Rupr. and identification of its polyphenolic composition by tandem mass spectrometry (HPLC-MS / MS)

“…The identification of compounds (m/z values and fragmented ions) was carried out by comparing the obtained experimental data with known scientific results or mass spectrometric libraries. Anthocyanins have been identified in the extracts: Malvidin [13,24,25,29,41,42,45,51,54,56,57,60,62,64,65,68,70,74]; flavones: Apigenin, Luteolin, Nevadensin, Apigenin-7-O-glucoside, Isovitexin 6"-O-deoxyhexoside, Vitexin 2"-O-glucoside, Apigenin 6-C-[6"-acetyl-2"-O-deoxyhexoside]-glucoside [13,15,17,34,43,44,45,58,60,67,69]; flavanones: Naringenin, Hesperitin, Eriodictyol-7-O-glucoside [16,29,42,53,60,62,63]; Flavan-3-ols: Catechin, Epicatechin [13,25,29,46,53,57,60].…”