The effect of adding Agaricus blazei to the diet of broilers on immunity, serum parameters, and antioxidant activity was evaluated. A total of 840 1-day-old chicks were used, distributed among five levels of a completely randomized design (0.0, 0.05, 0.10, 0.15, and 0.20% A. blazei), with six replications and 28 birds per experimental unit. The weights of the thymus, spleen and cloacal bursa were not influenced (P > 0.05). Leukocytes, macrophages and nitric oxide were unaffected (P > 0.05), but at each supplementation level compared with the control, differences appeared in the percentages of eosinophils and macrophages (P < 0.05) at inclusion levels of 0.10, 0.15 and 0.20%. At 28 days, the antibody titer against Newcastle disease showed a quadratic response (P < 0.05) with supplementation, and from the estimated level of 0.08% the production of antibodies was stimulated; however, the same behavior was not observed (P > 0.05) at 42 days. Hypocholesterolemic effect was demonstrated (P < 0.05), but the serum triglyceride concentration was not affected (P > 0.05). The antioxidant activity of mushroom showed a positive linear effect (P < 0.05) on DPPH capture on day zero of meat cooling. The inclusion of A. blazei in the diet of broilers provided an immunostimulatory activity and hypocholesterolemic effect. Residual compounds with antioxidant activity were present in the meat, which may promote tissue protection of the animal in vivo, making possible the use of A. blazei as a natural additive. Key words: Cholesterol. Free radical. Immunoglobulins. Leukocytes. Mushroom.
ResumoAvaliou-se o uso do Agaricus blazei na alimentação de frangos de corte sobre a imunidade, parâmetros séricos e a atividade antioxidante. Utilizou-se 840 pintos distribuídos em delineamento experimental inteiramente casualizado, cinco níveis (0,0; 0,05; 0,10; 0,15 e 0,20%) de Agaricus blazei, seis repetições e 28 aves por unidade experimental. O peso do timo, baço e bolsa cloacal não foi influenciado pelos tratamentos experimentais (P>0,05). Os leucócitos, macrófagos e o óxido nítrico não foram afetados pelos níveis de Agaricus blazei (P>0,05), no entanto a porcentagem de eosinófilo e de macrófagos diferenciaram-se (P<0,05) nos níveis de 0,10, 0,15 e 0,20% em relação ao controle. Aos 28 dias, os níveis de anticorpos contra a doença de Newcastle apresentaram resposta quadrática (P<0,05) à suplementação, e a partir do nível estimado de 0,08% estimulou-se a sua produção, contudo o mesmo