Comparison of Apricot Genotypes With Different Resistance Level to Plum Pox Virus (Ppv)

Fuchs, E.; Grüntzig, Maria; Ernst, I.; Kegler, H.

doi:10.17660/actahortic.2001.550.12

Cited by 11 publications

(11 citation statements)

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“…On the other hand, Fuchs et al (1998) observed symptoms on leaves using chips as an inoculation method, which in out studies never happens. In another study carrried out under greenhouse conditions with five isolates Fuchs et al (2001) detected PPV in some leaves, 24 weeks after double grafting, from cultivars that they classified as 'qualitatively resistant,' which included 'Goldrich' and 'Stark Early Orange'. These results did not contradict the classification of 'Goldrich' as a cultivar 'resistant' to PPV (Dosba et al 1991;Karayiannis and Mainou 1994).…”

Section: Sharka Resistance Traitmentioning

confidence: 99%

Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.), and mapping of PPV (sharka) resistance

et al. 2002

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Genetic linkage maps for two apricot cultivars have been constructed using AFLP, RAPD, RFLP and SSR markers in 81 F1 individuals from the cross 'Goldrich' x 'Valenciano'. This family segregated for resistance to 'plum pox virus' (PPV), the most-important virus affecting Prunus species. Of the 160 RAPD arbitrary primers screened a total of 44 were selected. Sixty one polymorphic RAPD markers were scored on the mapping population: 30 heterozygous in 'Goldrich', 19 heterozygous in 'Valenciano', segregating 1:1, and 12 markers heterozygous in both parents, segregating 3:1. A total of 33 and 19 RAPD markers were mapped on the 'Goldrich' and 'Valenciano' maps respectively. Forteen primer combinations were used for AFLPs and all of them detected polymorphism. Ninety five markers segregating 1:1 were identified, of which 62 were heterozygous in the female parent 'Goldrich' and 33 in the male parent 'Valenciano'. Forty five markers were present in both parents and segregated 3:1. A total of 82 and 48 AFLP markers were mapped on the 'Goldrich' and 'Valenciano' maps. Twelve RFLPs probes were screened in the population, resulting in five loci segregating in the family, one locus heterozygous for 'Valenciano' and four heterozygous for both, segregating 1:2:1. Of the 45 SSRs screened 17 segregated in the mapping family, resulting in seven loci heterozygous for the maternal parent and ten heterozygous for both, segregating 1:2:1 or 1:1:1:1. A total of 16 and 13 co-dominant markers were mapped in the female and male parent maps respectively. A total of 132 markers were placed into eight linkage groups on the 'Goldrich' map, defining 511 cM of the total map-length. The average distance between adjacent markers was 3.9 cM. A total of 80 markers were placed into seven linkage groups on the 'Valenciano' map, defining 467.2 cM of the total map-distance, with an average interval of 5.8 cM between adjacent markers. Thirty six marker loci heterozygous in both parents revealed straightforward homologies between five linkage groups in both maps. The sharka resistance trait mapped on linkage group 2. The region containing sharka resistance is flanked by two co-dominant markers that will be used for targeted SSR development employing a recently constructed complete apricot BAC library. SSRs tightly linked to sharka resistance will facilitate MAS in breeding for resistance in apricot.

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Section: Sharka Resistance Traitmentioning

confidence: 99%

Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.), and mapping of PPV (sharka) resistance

et al. 2002

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“…They have been considered "immune" to PPV by Fuchs et al 2001, after artificial inoculations and polymerase chain reaction tests, independently of the PPV isolates used. The mechanism of resistance inherited by their hybrids is probably related to resistance to virus movement.…”

Section: Discussionmentioning

confidence: 99%

“…Apricots and plums are particularly susceptible to both D and M strains of the virus. Representatives of the M strain, used in experiments for comparison of apricot genotypes in Germany, did not prove to be more aggressive than isolates of the D strain (Fuchs et al 2001). Nemeth 1986 described typical PPV-induced symptoms.…”

Section: Introductionmentioning

confidence: 87%

Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.)

Karayiannis

Thomidis

Tsaftaris

2007

Tree Genetics & Genomes

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The inheritance of resistance to Plum pox virus (PPV) has been studied in 1,178 apricot hybrids. Seven hundred and eighteen F1 hybrids, obtained from controlled crosses between the susceptible Greek cultivar "Bebecou" and the resistant PPV cultivars of American origin ("Stark Early Orange," 'NJA2," 'Veecot," "Sunglo," "Harlayne," and "Orangered") were evaluated for resistance to the PPV-M (Marcus) strain, 8 years after artificial inoculation. The inheritance of resistance to PPV has been additionally studied for the first time in a BC 1 population of 95 apricot hybrids for four vegetative periods. Reaction of each hybrid to PPV-M was scored through visual symptoms, indexing onto GF-305 and double-antibody sandwich enzyme-linked immunosorbent assay tests. Segregation within the hybrids, determined by Chisquared analysis, fits a 1:1 ratio (P≤0.05) of the resistant vs susceptible, indicating that resistance to PPV is controlled by a single dominant gene locus and that the above six resistant cultivars are heterozygous for the trait. Plants carrying this gene may initially develop disease symptoms on leaves but eventually recover and no virus can be detected in leaves. Susceptible plants show similar symptoms initially but remain symptomatic. Inheritance of resistance to PPV also has been studied in 365 F1 hybrids by crossing the resistant cultivar "Stella" with the susceptible "Bebecou" and the resistant cultivars "Sunglo" and "NJA2," for 8 years after inoculation. The segregation ratio was 1:0 (resistant/susceptible) suggesting that "Stella" is homozygous for the resistance trait. The purpose of this work was the enhancement of the knowledge of inheritance of resistance to PPV for the selection of new cultivars.

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“…Characterization of the subgroup variability of Greek PPV isolates revealed a prevalence of the M (Marcus) strain (Thomidis & Karayiannis 2003;Varveri et al 2004). Samples of the M strain used in experiments for comparison of apricot genotypes showed similar aggressiveness to D (Dideron) strain isolates (Fuchs et al 2001). The deployment of natural genetic resistance against virus infection offers the potential to counter plant virus diseases in the future.…”

Section: Introductionmentioning

confidence: 94%

Reaction of an F1 apricot hybrid population to artificial and natural inoculation of Plum pox virus

Karayiannis

Thomidis

Michailides

et al. 2010

New Zealand Journal of Crop and Horticultural Science

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The reaction to plum pox virus (PPV) inoculation is investigated in 163 F 1 hybrids 'Polonais' x 'Stark Early Orange', susceptible and resistant to 'Sharka' disease, respectively. The hybrids are inoculated with PPV by grafting and planting in the field where the disease is endemic. Visual symptoms of 'Sharka' on the leaves of the hybrids are recorded eight months post-inoculation as well as in the second and fourth year. Mild disease symptoms are recorded in almost all hybrids in the first year. The ratio of hybrids presenting symptoms versus those not presenting is 9:1. This ratio changes to 1:1 in the second year and remains stable in the fourth year. The ELISA test applied to the leaves of the asymptomatic hybrids is negative, confirming the monogenic control of resistance to PPV conferred by cv. 'Stark Early Orange'. The phenomenon of 'recovery' from infection accounts for the reduction in infection levels after the first year.

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Comparison of Apricot Genotypes With Different Resistance Level to Plum Pox Virus (Ppv)

Cited by 11 publications

References 0 publications

Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.), and mapping of PPV (sharka) resistance

Genetic linkage maps of two apricot cultivars (Prunus armeniaca L.), and mapping of PPV (sharka) resistance

Inheritance of resistance to Plum pox virus in apricot (Prunus armeniaca L.)

Reaction of an F1 apricot hybrid population to artificial and natural inoculation of Plum pox virus

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