Comparison of ARDRA and recA-RFLP analysis for genomic species identification of Acinetobacter spp.

Abstract

The genus Acinetobacter is subdivided into genospecies on the basis of DNA relatedness of strains. Phenotypic tests are insufficient to identify the genospecies to which an isolate belongs. The effectiveness of two previously described PCR-based methods for genospeciating Acinetobacter spp. was compared using a group of 32 well-characterised strains representing six genospecies. Amplified ribosomal DNA restriction analysis (ARDRA) correctly identified all 32 strains. Using restriction fragment length polymorphism (RFLP) of recA PCR amplimers, only six of the 32 strains were correctly identified. Heterogeneity in the recA gene sequence was demonstrated within five of the genospecies. ARDRA proved to be a reliable method whereas analysis of recA RFLP profiles did not enable the genospecies of most of the isolates of Acinetobacter spp. to be determined. z

Distinct Antimicrobial Resistance Patterns and Antimicrobial Resistance-Harboring Genes According to Genomic Species of Acinetobacter Isolates

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Distribution of microbial communities in Guiyu soils and sediments investigated by 16S rRNA gene library and denaturing gradient gel electrophoresis (DGGE) fingerprinting

“…Using 16S rRNA gene library method might get more taxonomic status information about bacteria. If combined with amplified ribosomal DNA restriction analysis (ARDRA), restriction fragment length polymorphism (RFLP) and other technology, it would achieve better results (Jawad et al, 1998;Krizova et al, 2006).…”

Dehydrated pork manure by-product: effect of a chitosan amendment on bacterial community and common scab incidence

“…Bacteria of the Acinetobacter genus are widely distributed in nature and could be isolated from soil, water and human skin (Jawad et al 1998). In activated sludge systems, they participated in the removal of phosphorus (Beachman et al 1990;Mino et al 1998).…”