Comparison of arterial and venous patency in a rat model of subendothelium-stimulated thrombosis

Abstract

The effectiveness of anticoagulants and platelet aggregation inhibitors was compared using comparable rat models of arterial and venous thrombosis. A mechanical endothelium-denuding injury was created on the lumenal surface of donor Lewis rat carotid arteries. These were cut into 4-5 mm lengths and grafted into femoral veins and arteries of recipient syngeneic rats using microvascular anastomotic techniques. Recipients received either systemic heparin, or aspirin with dipyridamole, or saline (control). In the arteries, the 1-day patency rate was 94% in the heparin-treated rats, but only 50% in the aspirin/dipyridamole group and 44% in the control group. The venous patency rate was 56% in the heparin group, 31% in the aspirin/dipyridamole group, and 0% in the control group. This unique model for comparing thrombosis in arteries and veins shows that anticoagulation is more effective than inhibition of platelet aggregation in the rat arterial system, with less of a differential effect in the venous system.

Comparison of the effect of coagulation and platelet function impairments on various mouse bleeding models

“…While there are examples to the contrary (33,34), platelets are generally thought to be more important in the pathogenesis of arterial thrombosis than venous thrombosis, owing partly to the higher shear in arteries (35)(36)(37). The same is believed to be true in injury-induced thrombosis models (36).…”

Successful Salvage of Venous Thrombosis of a Free Flap by Creation of a Venocutaneous Fistula

“…Several studies have demonstrated that heparin significantly increases arterial and venous patency and decreases thrombotic occlusion in rat femoral vessels. [11][12][13] Experimental evidence suggests that low-molecular-weight heparin significantly improves anastomotic patency and flap perfusion in animals, 14 but this has not been demonstrated in humans. In a prospective cohort study of 505 free flaps for oncologic reconstruction, a single intraoperative dose of systemic heparin was found to have no statistically significant effect on the incidence of microvascular thrombosis or on the incidence of hematoma.…”

“…8,9 Systemic therapies including aspirin, heparin, low-molecular-weight heparin, and dextran have been used with varying degrees of success. [10][11][12][13][14][15] When conservative measures fail, surgical reexploration allows evacuation of any hematoma, unkinking of a twisted pedicle, thrombectomy, and revision of the anastomosis either directly or by using an interposition vein graft. Alternatively, catheter infusion of thrombolytic agents may clear thrombosis within the venous outflow system.…”

Adjonction d’antithrombotiques in situ en cas de replantation digitale : étude prospective préliminaire de 13 cas

“…Cependant, plusieurs études ont montré la plus grande efficacité de l'héparine sur l'aspirine dans la prévention de thrombose artérielle ou veineuse [14]. En effet, l'héparine serait plus efficace sur l'inhibition de l'agrégation plaquettaire par rapport à l'association aspirine/dipyridamole dans la circulation artérielle, sans différence au niveau de la circulation veineuse [15]. Aux côtés de l'héparine et de l'aspirine, de nouveaux produits ont été testés : Lykoudis et al [16] [17].…”