Comparison of artificial diets for rearing Bracon hebetor Say (Hymenoptera: Braconidae)

Magro, Sandra; Parra, José Roberto Postali

doi:10.1016/j.biocontrol.2003.07.001

Cited by 16 publications

(15 citation statements)

References 23 publications

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“…After 24h, parasitized larvae were removed from the vials, transferred to petri dishes (6 x 2 cm), and kept under controlled conditions (25 ± 2ºC, 70 ± 10% RH, and 14h photophase), until adult emergence. Part of the eggs obtained from parasitized A. kuehniella were transferred to the artificial diet according to Magro & Parra (2004).…”

Section: Methodsmentioning

confidence: 99%

“…The holotissues macerate was collected into an appropriate container, centrifuged (4000 rpm x 3 min), and the supernatant collected and stored at -18ºC for further use. All steps for diet preparation and manipulation were conducted in a laminar flow hood to provide for the required asepsis (Magro & Parra 2004). …”

Section: Methodsmentioning

confidence: 99%

“…As an attempt to develop an alternative system to mass rear this parasitoid, an artificial diet was devised which yielded 40% of adults as compared to the 70% obtained when B. hebetor was reared on the natural host, which were similar in size and displayed the same parasitism and host handling behavior (Magro & Parra 2004). Besides the lower larval survivorship, 60% of the in vitro-reared pupae lacked the protective cocoon, which was hypothesized to be due to the low protein content of the artificial diet.…”

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confidence: 99%

“…Therefore, we aim to understand the immature biology o of B. hebetor and its nutrient acquisition behavior, and to detect proteins and lipids in the insect's digestive tract, based on comparative histochemical studies of in vitro and in vivoreared larvae (following Magro & Parra 2004). This is a decisive step to understand both the nutritional benefits of this parasitoid's natural diet as means to improve its artificial diet, and to use the information as a model for rearing other parasitoids.…”

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confidence: 99%

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Biological, nutritional, and histochemical basis for improving an artificial diet for Bracon hebetor Say (Hymenoptera: Braconidae)

Magro¹,

Dias²,

Terra³

et al. 2006

Neotrop. Entomol.

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Neotropical Entomology 35(2): 215-222 (2006) Bases Biológicas, Nutricionais e Histoquímicas para o Aprimoramento de Dieta Artificial para Bracon hebetor Say (Hymenoptera: Braconidade) PALAVRAS-CHAVE: Controle biológico, ácido graxo, idiobionte, exigência nutricional, proteínaABSTRACT -The biology of Bracon hebetor Say (Hymenoptera: Braconidae) reared on fifth instars of Anagasta kuehniella (Zeller) (Lepidoptera: Pyralidae) (natural diet) and in vitro (artificial diet) was evaluated. Data on the number of instars, development time and food intake were collected, and histochemical tests were conducted to detect proteins and lipids in the parasitoid's digestive tract. The data disclosed differences that can help to improve artificial rearing of B. hebetor. B. hebetor had three instars in both diets, but the developmental time on the artificial diets was prolonged due to the increase in larval and pupal development times. Larvae grew faster on the natural host and required a lower food intake (2.7 μl) as compared to that required by the larvae feeding on the artificial diet (3.8 μl). Analysis of diet protein content and host hemolymph and the observations on the parasitoid larvae gut content indicated altogether the artificial diets requires the addition of others sources of proteins and lipids to improve the overall nutrition quality of the in vitro rearing system for this ectoparasitoid.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Biological, nutritional, and histochemical basis for improving an artificial diet for Bracon hebetor Say (Hymenoptera: Braconidae)

Magro¹,

Dias²,

Terra³

et al. 2006

Neotrop. Entomol.

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“…The introduction of methods for in vitro insect rearing using artificial diets created high expectations during the 1980s, particularly after the successful in vitro production of Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) (Li-Ying et al, 1988) by Chinese researchers. Although no significant advances were made in world technology for in vitro insect rearing, some promising results were obtained in Brazil for Trichogramma pretiosum Riley, T. galloi (Cônsoli and Parra, 1999) and Trichogramma atopovirilia Oatman and Platner (Dias et al, 2010) as well as for the ectoparasitoid Bracon hebetor Say (Hymenoptera: Braconidae) (Magro and Parra, 2004).…”

Section: -Foundation Of the Entomologicalmentioning

confidence: 99%

Biological control in Brazil: An overview

Parra

2016

2016 International Congress of Entomology

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The use of Biological Control methods is on the increase, mainly as a result of the mobilization of human resources in entomology studies since the establishment of graduate programs in this country in the 1960s. This review approaches the retrospective of Biological Control in Brazil in recent decades, with an emphasis on the "culture of applying agrochemicals" adopted by Brazilian growers, which constrains progress in this area. Successful cases of Biological Control have been reported on in Brazil and there are, in fact, excellent programs in place that use insects or entomopathogenic microrganisms for insect pest control. Most of the studies in this area have been published in Portuguese and are, therefore, not readily available internationally. Importantly, half of the planted sugarcane (Saccharum officinarum), around four million hectares, is treated with natural enemies (insects) and/or pathogens. In contrast to other countries that employ Biological Control in small areas, the challenge in Brazil is to implement programs in large farms. Many obstacles must be overcome and discussed in working groups so that we can assume a world leadership position in the use of Biological Control in tropical regions as Brazil is already considered the leader in tropical agriculture. In this review, use of Biological Control is discussed within the Integrated Pest Management philosophy, as a path toward sustainable agriculture that is in harmony with other pest control methods. We must develop a technology of Biological Control adapted to tropical regions, rather than copying models developed for temperate regions, which are usually inappropriate for Brazilian conditions.

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2014

The Braconid and Ichneumonid Parasitoid Wasps

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Comparison of artificial diets for rearing Bracon hebetor Say (Hymenoptera: Braconidae)

Cited by 16 publications

References 23 publications

Biological, nutritional, and histochemical basis for improving an artificial diet for Bracon hebetor Say (Hymenoptera: Braconidae)

Biological, nutritional, and histochemical basis for improving an artificial diet for Bracon hebetor Say (Hymenoptera: Braconidae)

Biological control in Brazil: An overview

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