Comparison of attachment and proliferation of human gingival fibroblasts on different collagen membranes

Ardakani, Mohammad Reza Talebi; Hajizadeh, Farhad; Yadegari, Zahra

doi:10.4103/ams.ams_150_17

Cited by 12 publications

(4 citation statements)

References 39 publications

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“…The limited number of flat polyhedral cells with long cytoplasmic extensions attached to the membrane surfaces may have been caused by the short incubation duration, which was 3 days in this experiment; another reason may be that cells were lost in the process of preparation and fixation. Similar results were observed in another experiment that involved a crosslinked membrane [35]. The AlamarBlue viability assay was used to assess the viability and proliferation of the cultured cells on the collagen membranes using a migration assay assembly.…”

Section: Discussionsupporting

confidence: 68%

Can Vitamin C Improve Proliferation and Viability of Smokers’ Gingival Fibroblasts on Collagen Membranes? An In Vitro Study

Alshehri

2023

Applied Sciences

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Periodontal regeneration using a barrier membrane can be affected by several factors, including patient-related factors (such as smoking habits), surgical techniques, and type of barrier membrane. Smoking exposure has a negative impact on the periodontium due to its direct inhibition of gingival fibroblast function. Vitamin C is widely recognized as an antioxidant that can be used to mitigate the detrimental impact of smoking products on periodontal cells. This study aimed to investigate whether vitamin C could improve the proliferation and viability of gingival fibroblasts extracted from smoking and non-smoking donors and then cultured on non-crosslinked (CopiOs Pericardium) and crosslinked (BioMend) collagen membranes. To address this aim, human gingival fibroblasts were extracted from healthy periodontium of smoker patients (Group 1) and non-smoker patients (Group 2). The cells were cultivated and subsequently subcultured in a growth medium supplemented with the required nutrients. Subsequently, the medium at passage six was supplemented with vitamin C, i.e., at the start of the experiment. An evaluation of cell proliferation and viability was carried out using cell migration assays and AlamarBlue® assays for cells grown on BioMend and CopiOs Pericardium collagen membranes. Assessment of the morphology and attachment of gingival fibroblasts to the experimental collagen membranes was conducted using scanning electron microscopy (SEM). The viability and proliferation assessments of hGFs from the migration assay were evaluated using AlamarBlue®. The results exhibited significant fluorescent intensity of gingival fibroblasts on both membrane groups (BioMend and CopiOs Pericardium) in the smoker group compared to the non-smoker group (p < 0.05), which was interpreted to be the result of hGF metabolic activity and the exclusion of any cytotoxic effects, particularly from vitamin C addition. Vitamin C positively affected cells from the smoker group with statistically significant results in the BioMend group (Wilcoxon signed-rank test of p value < 0.05; p = 0.028). SEM images revealed the crosslinking pattern of the BioMend membrane and the non-crosslinked natural tissue structure of the CopiOs Pericardium membrane, which did not change regardless of whether the cultured smoker or non-smoker hGFs were treated with vitamin C. Small numbers of attached hGFs in membrane matrices in all samples, mainly in the peripheries, were observed. It can be concluded that the addition of vitamin C to collagen membranes in vitro seems to combat the adverse effects of smoking products on gingival fibroblasts.

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Section: Discussionsupporting

confidence: 68%

Can Vitamin C Improve Proliferation and Viability of Smokers’ Gingival Fibroblasts on Collagen Membranes? An In Vitro Study

Alshehri

2023

Applied Sciences

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“…Despite this, there were numerous other studies that employed even more complex collagen matrices than a simple coating, confirming the positive effect of this structural protein on the growth, vitality, and migration of fibroblasts [ 51 , 52 , 53 , 54 , 55 , 56 ].…”

Section: Discussionmentioning

confidence: 96%

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Romasco

Mandrillo²,

Morsut

et al. 2023

Biomedicines

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Maintaining periodontal and peri-implant soft tissues health is crucial for the long-term health of teeth and dental implants. New biomedical strategies aimed at avoiding connective tissue alterations and related diseases (e.g., periodontitis and peri-implantitis) are constantly evolving. Among these, collagen-based medical products have proven to be safe and effective. Accordingly, the aim of the present study was to evaluate the effects of Dental SKIN BioRegulation (Guna S.p.a., Milan, Italy), a new injectable medical device composed of type I collagen of porcine origin, on primary cultures of human gingival fibroblasts (hGF). To this end, hGF were cultured on collagen-coated (COL, 100 µg/2 mL) or uncoated plates (CTRL) before evaluating cell viability (24 h, 48 h, 72 h, and 7 d), wound healing properties (3 h, 6 h, 12 h, 24 h, and 48 h), and the activation of mechanotransduction markers, such as FAK, YAP, and TAZ (48 h). The results proved a significant increase in cell viability at 48 h (p < 0.05) and wound closure at 24 h (p < 0.001) of hGF grown on COL, with an increasing trend at all time-points. Furthermore, COL significantly induced the expression of FAK and YAP/TAZ (p < 0.05), thereby promoting the activation of mechanotransduction signaling pathways. Overall, these data suggest that COL, acting as a mechanical bio-scaffold, could represent a useful treatment for gingival rejuvenation and may possibly help in the resolution of oral pathologies.

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“…A scaffold that is suitable for biomedical applications must be highly porous, interconnected, and have a fibrous structure that supports biological processes, including cell growth, migration, and differentiation [ 16 ]. Previous in-vitro studies [ 10 , 26 ] demonstrated that fibroblast cells are able to adhere to collagen membrane for seven days; hence, the membrane needs to be intact and remain stable within the same duration after the surgical procedure to allow the establishment of the periodontal ligament cell attachment and proliferation [ 18 ]. This study demonstrated that there are significant changes in AM microstructures after 28 days of hydrolytic degradation test.…”

Section: Discussionmentioning

confidence: 99%

“…On the other hand, a study by Ardakani et al found that the viability of fibroblast cells cultured on the cross-linking membrane reduced after seven days of observation when compared with native collagen membranes [ 26 ]. It can be assumed that the cross-linking of collagen membranes may somewhat affect cell attachment despite increasing the membrane degradation time.…”

Section: Discussionmentioning

confidence: 99%

Biodegradability of Amniotic Membrane as Potential Scaffold for Periodontal Regeneration

Ling,

Roslan,

Taib

et al. 2023

Cureus

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Background In the periodontal regenerative procedure, the membrane used should possess good mechanical stability with suitable resorption time to allow restoration of the lost periodontium. Amniotic membrane (AM) has regenerative potential as a scaffold or barrier membrane due to its various beneficial properties. However, its degradation rate is not clearly reported. Methodology This study aimed to evaluate the resorption capacity of AM and its surface architecture after being subjected to hydrolytic degradation analysis in phosphate buffer solution (PBS). AM was cut into sizes of 10 × 10 mm 2 for three replicates. The membranes were weighed before and at different time intervals (days 7, 14, 21, and 28) after immersion in PBS. The degradation rate was determined by the percentage of mean weight loss from the initial weight at different time intervals. The AM surface profile was observed under scanning electron microscopy (SEM) before and after 28 days of immersion. Results The result shows a 92% loss of weight over 28 days with the highest attained in the first seven days (67%), followed by 7%, 17%, and 1% after days 14, 21, and 28, respectively. SEM of the AM surface before the degradation test showed a polygonal shape forming a well-arranged mosaic pattern covered with microvilli. At day 28, the remaining AM appears as porous surface architecture, irregularly arranged fibers, and no microvilli seen. Conclusions This study demonstrated that over four weeks of degradation analysis, AM was not entirely degraded but had lost some of the microstructure. The biodegradability of AM should be further evaluated to elucidate its stability within adequate time parallel with the tissue healing process in periodontal tissue regeneration.

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Comparison of attachment and proliferation of human gingival fibroblasts on different collagen membranes

Cited by 12 publications

References 39 publications

Can Vitamin C Improve Proliferation and Viability of Smokers’ Gingival Fibroblasts on Collagen Membranes? An In Vitro Study

Can Vitamin C Improve Proliferation and Viability of Smokers’ Gingival Fibroblasts on Collagen Membranes? An In Vitro Study

Morpho-Functional Effect of a New Collagen-Based Medical Device on Human Gingival Fibroblasts: An In Vitro Study

Biodegradability of Amniotic Membrane as Potential Scaffold for Periodontal Regeneration

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