Comparison of Austrian, Hungarian and Macedonian methicillin-resistant and methicillin-sensitive Staphylococcus aureus strains in relation to prevalence of cytotoxin genes

Kocsis, Erika; Lagler, Heimo; Pesti, N.; Stich, Karin; Kristóf, Katalin; Nagy, K.; Hermann, Péter; Komka, Kinga; Cekovska, Zaklina; Graninger, Wolfgang; Rozgonyi, Ferenc

doi:10.1016/j.micpath.2009.04.002

Cited by 10 publications

(7 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is contrasted the results obtained by Kocsis et al (Kocsis et al, 2009) who reported a high prevalence rate of such genes among MRSA strains, isolated from Austria and Hungry. Although the genes encoding haemolysin toxins are located on chromosomes, the prevalence of these genes can be reduced by acquiring movable genes on genetic elements.…”

Section: Resultscontrasting

confidence: 85%

Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec type III

Emaneini

Jabalameli²,

Iman‐Eini³

et al. 2011

Pol J Microbiol

View full text Add to dashboard Cite

Methicillin resistant Staphylococcus aureus (MRSA), particularly strains with type III staphylococcal cassette chromosome mec (SCCmec), represent a serious human pathogen in Tehran, Iran. The disease-causing capability depends on their ability to produce a wide variety of virulent factors. The prevalence of exotoxin genes and multiple-locus variable number of tandem repeats fingerprinting (MLVF) profile among MRSA isolates, from patients in Tehran, was evaluated by PCR and Multiplex-PCR. The MLVF typing of 144 MRSA isolates with type III SCCmec produced 5 different MLVF types. Generally, 97.2% (140/144) of all the isolates were positive for at least one of the tested exotoxin genes. The most prevalent genes were hld, found in 87.5% (126/144) of the isolates followed by lukE-lukD and hla found in 72.9% (105/144) and 70.1% (101/144) of the isolates, respectively. The tst gene, belonging to MLVF types I, IV and V, was found among three of the isolates from blood and wound samples. The sea gene was detected in 58.3% (84/144) of the isolates and the sed and see genes were found in one isolate with MLVF type V. The coexistence of genes was observed in the 87.5% (126/144) of the isolates. The rate of coexistence of hld with lukE-lukD, hla with lukE-lukD and sea with lukE-lukD were 66.7% (96/144), 44.4% (64/144) and 44.4% (64/144), respectively. The present study demonstrated that MRSA strains with type III SCCmec show different MLVF patterns and exotoxin profiles.

show abstract

Section: Resultscontrasting

confidence: 85%

Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec type III

Emaneini

Jabalameli²,

Iman‐Eini³

et al. 2011

Pol J Microbiol

View full text Add to dashboard Cite

show abstract

“…The prevalence of the PVL genes in this study was unexpectedly higher than reported studies in other foreign hospitals. Some studies from Czech Republic, China, Trinidad and Tobago, and Austria and Hungary reported 0 % prevalence of PVL genes in all confirmed MRSA isolates [ 47 , 48 , 51 , 52 ]. PVL is a cytolytic toxin comprised of F and S subunits which are encoded by the lukPV operon, which contains the lukF - lukS genes.…”

Section: Discussionmentioning

confidence: 99%

Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates from a tertiary hospital in the Philippines

et al. 2016

View full text Add to dashboard Cite

BackgroundMethicillin-resistant Staphylococcus aureus (MRSA) poses a major threat to public health worldwide. There are relatively few studies addressing the molecular epidemiology of MRSA in the Philippines.MethodsThis study characterized MRSA isolates in terms of their antimicrobial susceptibility profile, the SCCmec type, and the presence of lukF-lukS genes for Panton-Valentine leukocidin (PVL) and determined the relatedness of the isolates by random amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR).ResultsA total of 236 S. aureus were isolated from clinical specimens of the Makati Medical Center in Makati City, Philippines, between January 2013 and June 2013, and 108 or 45.76 % were found to be MRSA. Results showed that the MRSA strains were resistant to trimethoprim-sulfamethoxazole (20.37 %), azithromycin (10.19 %), gentamicin (5.56 %), and linezolid (4.63 %), while all were susceptible to vancomycin, nitrofurantoin, levofloxacin, minocycline, rifampin, and tetracycline. One isolate was found positive for inducible clindamycin resistance. All of the 108 MRSA strains were confirmed to carry the mecA and SCCmec genes, while the PVL genes were detected in 41 (38 %) of the isolates. Ninety-six isolates (89 %) carried SCCmec type IV, while the remaining isolates carried SCCmec type I (11 isolates) or type III (one isolate).ConclusionThis study is the first to present a comprehensive MRSA surveillance data with molecular characterization in a tertiary hospital in the Philippines.

show abstract

“…Presumptive positive colonies were confirmed as S. aureus by species-specific 23S rDNA PCR [6] and as MRSA by PCR detection of mecA gene [7], before further characterisation by multilocus sequence typing (MLST) [8], spa typing [9], and determination of staphylococcal cassette chromosome mec (SCCmec) type [10] was performed. Moreover, strains were tested by PCR for lukS-PV and lukF-PV [11] encoding Panton-Valentine leukocidin (PVL), and for sea to sed [12] encoding staphylococcal enterotoxins (SE) A to D. To demonstrate phenotypic properties, strains were tested for their antibiotic resistance patterns using the disk diffusion method (BD BBL Sensi-Disc; Becton, Dickinson and Company, Sparks, MD, US). The following disks were used: ampicillin, cefoxitin, ciprofloxacin, clindamycin, erythromycin, gentamicin, oxacillin, penicillin, rifampicin, sulphamethoxazole/trimethoprim, tetracycline, and vancomycin.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland, 2009

et al. 2010

View full text Add to dashboard Cite

A total of 2,662 samples, collected from March to September 2009 in Switzerland, were tested for the presence of meticillin-resistant Staphylococcus aureus (MRSA). The collection comprised nasal swabs from 148 pig farmers, 133 veterinarians, 179 slaughterhouse employees, 800 pigs, 300 calves, 400 cattle, 100 pooled neck skin swabs from chicken carcasses, and 460 food samples of animal origin. Moreover, 142 S. aureus strains, isolated from bovine mastitis milk, were included in the study. Twenty samples (<1%; four veterinarians, 10 pigs, three calves, one young bull, and two mastitis milk samples) tested positive for MRSA. Genotyping of the MRSA strains was performed by multilocus sequence typing, spa- and SCCmec-typing, and revealed ST398 (n=18), ST8 (n=1), ST 1 (n=1), spa types t011 (n=7), t034 (n=11), t064 (n=1), t127 (n=1), and SCCmec types IV (n=4) and V (n=16). The 20 MRSA strains were subjected to antibiotic susceptibility testing and pulsed-field gel electrophoresis using the restriction enzyme EagI. Supplementary PCR reactions were performed to investigate the presence of Panton-Valentine leukocidin and staphylococcal enterotoxins A to D.

show abstract

Comparison of Austrian, Hungarian and Macedonian methicillin-resistant and methicillin-sensitive Staphylococcus aureus strains in relation to prevalence of cytotoxin genes

Cited by 10 publications

References 34 publications

Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec type III

Multiple-Locus Variable Number of Tandem Repeats Fingerprinting (MLVF) and Virulence Factor Analysis of Methicillin Resistant Staphylococcus aureus SCCmec type III

Molecular and phenotypic characterization of methicillin-resistant Staphylococcus aureus isolates from a tertiary hospital in the Philippines

Prevalence and characteristics of meticillin-resistant Staphylococcus aureus in humans in contact with farm animals, in livestock, and in food of animal origin, Switzerland, 2009

Contact Info

Product

Resources

About