2020
DOI: 10.1155/2020/1858732
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Comparison of Bacterial Community Structure and Diversity in Traditional Gold Mining Waste Disposal Site and Rice Field by Using a Metabarcoding Approach

Abstract: Traditional small-scale gold mining mostly use mercury to extract the gold from ores. However, mercury contamination in the environment can affect the composition and structure of the bacterial community. The purpose of this study was to determine the effect of mercury contamination on the bacterial community in the traditional gold mining waste disposal site and in the rice field. Mercury analysis was carried out using the CVAFS method. Analysis of bacterial communities and structure was carried out based on … Show more

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Cited by 21 publications
(18 citation statements)
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“…The honeybee's gut was dissected under sterile conditions and homogenized in a FastPrep-24 Instrument at 4 m/s for 25 sec. The subsequent procedure was done according to Fatimawali et al [ 15 ]. The extraction of gut bacterial gDNA (genomic DNA) was performed using ZymoBiomics DNA Mini Kit (Zymo Research) following the manufacturer's protocol.…”
Section: Methodsmentioning
confidence: 99%
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“…The honeybee's gut was dissected under sterile conditions and homogenized in a FastPrep-24 Instrument at 4 m/s for 25 sec. The subsequent procedure was done according to Fatimawali et al [ 15 ]. The extraction of gut bacterial gDNA (genomic DNA) was performed using ZymoBiomics DNA Mini Kit (Zymo Research) following the manufacturer's protocol.…”
Section: Methodsmentioning
confidence: 99%
“…3.24 [ 17 ]. The alpha diversity was represented by dominance (D), Simpson (1 − D), Shannon-Wiener (H′), evenness (eH/S), Margalef (Dmg), and equitability ( J ) indices [ 15 ].…”
Section: Methodsmentioning
confidence: 99%
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“…QIIME (Version 1.7.0) software was used to calculate the alpha bacterial diversity obtained from the A. dorsata gut, which included Simpson (1-D) and Shannon-Wiener (H') indices (Fatimawali et al 2020).…”
Section: Alpha Diversity Analysismentioning
confidence: 99%
“…Many bacteria are found to be resistant to mercury and classified as MRB including Shigella flexneri, Pseudomonas putida, Pseudomonas stutzeri, P. fluorescens, P. aeruginosa (Fatimawali et Al. 2019;Imron et al 2019), Firmicutes, Planctomycetes (Fatimawali et al 2020), Klebsiella pneumoniae, Morganella morganii, Xanthomonas, Achromobacter spp., Sphingobium spp., Acinetobacter calcoaceticus, Serratia marcescens, Mycobacterium marinum, Bacillus spp., Enterobacter, Staphylococcus aureus, Sphingopyxis spp., Psychrobacter spp., Brevibacillus spp., Anoxybacillus spp., Luteimonas spp., and Geobacillus kaustophilus (Mahbub et al 2017), Brevundimonas spp. (Irawati et al 2012), Actinomycetes and Streptomycetes (Undabarrena et al 2017).…”
Section: Introductionmentioning
confidence: 99%