Comparison of bacteriolytic activity of human interleukin-2 and chicken egg lysozyme on Lactobacillus plantarum and Escherichia coli cells

Левашов, П. А.; Matolygina, D. A.; Osipova, H. E.; Савин, С. С.; Zaharova, G. S.; Gasanova, D. A.; Belogurova, N. G.; Ovchinnikova, E. D.; Smirnov, Sergey; Тишков, В. И.; Levashov, Andrey V.

doi:10.3103/s0027131415060048

Cited by 9 publications

(8 citation statements)

References 15 publications

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“…IL-2 acts on individual members of the Gram-negative family Enterobacteriaceae, including Ent. aerogenes and S. marcescens , as shown in our work, and, as previously established, on E. coli [ 1 - 3 ]. IL-2 is active against such Gram-positive members of the family Lactobacillaceae as L. acidophilus (current paper) and L. plantarum [ 3 ].…”

Section: Resultssupporting

confidence: 87%

“…aerogenes and S. marcescens , as shown in our work, and, as previously established, on E. coli [ 1 - 3 ]. IL-2 is active against such Gram-positive members of the family Lactobacillaceae as L. acidophilus (current paper) and L. plantarum [ 3 ]. It was also found that IL-2 acts on B. megaterium , B. mycoides, and B. cereus , Gram-positive spore-forming bacilli of the Bacillaceae family, which differ in cell wall structure and composition from the bacteria of the Enterobacteriaceae and Lactobacillaceae families.…”

Section: Resultssupporting

confidence: 87%

“…Activity optima for lysozyme and IL-2 are similar for B. mycoides and B. cereus (not presented on the graphs due to the similarity with the dependencies for B. megaterium ). A similar shift in lysozyme and IL-2 activity optima depending on the substrate (species of bacteria) was also observed in the case of E. coli and L. plantarum [ 3 ].…”

Section: Resultssupporting

confidence: 60%

“…This lymphokine is involved in the regulation of such processes as proliferation and differentiation of T lymphocytes, increase of the cytolytic activity of NK cells, proliferation of B lymphocytes, immunoglobulin secretion, etc. We have recently shown that human IL-2 is able to exhibit bacteriolytic activity [ 1 - 3 ]. A comparative test with several bacterial strains has shown that IL-2 has a narrower substrate specificity compared to hen egg white lysozyme.…”

Section: Introductionmentioning

confidence: 99%

“…A comparative test with several bacterial strains has shown that IL-2 has a narrower substrate specificity compared to hen egg white lysozyme. IL-2, as well as lysozyme, is capable of lysing Escherichia coli and Lactobacillus plantarum cells, but, unlike lysozyme, it shows no effect on Micrococcus luteus and Bacillus subtilis [ 1 - 3 ]. The detection of IL-2 activity against E. coli and L. plantarum turned out to be surprising.…”

Section: Introductionmentioning

confidence: 99%

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Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

et al. 2016

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The bacteriolytic activity of interleukin-2 and hen egg white lysozyme against 34 different species of microorganisms has been studied. It was found that 6 species of microorganisms are lysed in the presence of interleukin-2. All interleukin-2-sensitive microorganisms belong either to the Enterobacteriaceae, Bacillaceae, or the Lactobacillaceae family. It was also found that 12 species of microorganisms are lysed in the presence of lysozyme, and 16 species of microorganisms are lysed in the presence of sodium dodecyl sulfate (SDS). The bacteriolytic activity of interleukin-2 and lysozyme was studied at various pH values.

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Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 87%

Section: Resultssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

et al. 2016

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General characteristics of the influence of surfactants on the bacteriolytic activity of lysozyme based on the example of enzymatic lysis of Lactobacillus plantarum cells in the presence of Tween 21 and SDS

Smirnov

Левашов

2021

Biochemical and Biophysical Research Communications

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The antimicrobial potentialities of (Nk-lysin peptides of chicken, bovine, and human) against bacteria and rotavirus

Mahmoud¹,

Al-Hejin²,

Abujaml³

et al. 2021

Preprint

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For the first time, this study was carried out to investigate and evaluate the relative antibacterial activity of three different Nk-lysin peptides from human, chicken, and bovine activity compared to Gram-negative and Gram-positive bacteria as well as antiviral activity against rotavirus (strain SA-11) and finally mechanisms of action optionality. This report is the first of its kind that investigates the increased antimicrobial ability of (Nk-lysin + AgNPs) and (Nk-lysin + human IL-2) combinations against S. typhi activity by carrying out direct comparison under similar experimental settings. Our results showed that gram-negative and gram-positive microorganisms, including Streptococcus pyogenes, Streptococcus mutans, Escherichia coli, Pseudomonas aeruginosa, Klebsiella oxytoca, Shigella sonnei, Klebsiella pneumoniae and Salmonella typhimurium, are susceptible to NK-lysin treatment. It was shown in our findings that there was equal potentiality in mixture (Nk-lysin + AgNPs) and (Nk-lysin + human IL-2) for preventing the growth of S. typhi, however, when added together, there was minor increase in the level of action. In our study, the TOHO-1 gene was absent in treated bacteria. Following treatment with Nk-lysin peptides, the beta-lactamases genes (CTX-M-1, M-8, and M-9) were not found in any bacterial strains. The examination did not find any of the plasmid mediated quinolone resistance genes in the bacterial strains as a response to NK-lysin treatment. Nonetheless, no study has been carried out in the past that characterized the antiviral activity of bovine, human and chicken Nk-lysin peptides. Hence, this is the foremost study on the enhanced antimicrobial activity of human, bovine and chicken Nk-lysin peptides against Rotavirus (strain SA-11). The findings of the study demonstrated that the powerful antiviral activities were exhibited by Nk-lysin peptides against Rotavirus (strain SA-11). Based on the comparison between these peptides, it can be concluded that there is an evident potent antiviral activity of bovine Nk-lysin against Rotavirus (strain SA-11) as it restrains infection by up to 90%. However, growth was restricted by 80% by chicken Nk-lysin and by 50% by the human peptide.

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Comparison of bacteriolytic activity of human interleukin-2 and chicken egg lysozyme on Lactobacillus plantarum and Escherichia coli cells

Cited by 9 publications

References 15 publications

Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

Human Interleukin-2 and Hen Egg White Lysozyme: Screening for Bacteriolytic Activity against Various Bacterial Cells

General characteristics of the influence of surfactants on the bacteriolytic activity of lysozyme based on the example of enzymatic lysis of Lactobacillus plantarum cells in the presence of Tween 21 and SDS

The antimicrobial potentialities of (Nk-lysin peptides of chicken, bovine, and human) against bacteria and rotavirus

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