Comparison of Barricor tube and serum separator tube in outpatients

Abstract

Objectives In clinical laboratories, it is common to obtain serum and plasma by using a barrier tube due to its various advantages. In this study, we aimed to compare 18 biochemistry analytes in outpatient clinic and outpatient oncology patients by measuring in a Barricor tube and a serum separator tube (SST). Methods Venous blood was drawn into the Barricor tube and SST from volunteers consisting of outpatients and outpatient oncology patients. The biochemical parameters were measured using the AU2700 autoanalyser (Beckman Coulter Inc., CA, USA) and Beckman Coulter Access immunoanalyser (Beckman Coulter Inc., CA, USA). The biochemical analytes evaluated in the two participant groups were compared between the Barricor tube and SST. Results In the study, when the results in both the outpatient group and the outpatient oncology patient group, bias (%), allowable bias (%), CV (%), allowable CV (%), total error (%), total allowable error (TEa) (%) were evaluated; potassium (K) total error (%) between Barricor tube and SST exceeded TEa, however, all other parameters were within TEa. Conclusions Considering its various advantages and compared biochemical analytes, we think that can be switched to the Barricor tube in clinical laboratories and the reference range change can be made for K.

The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory

“…Since they found clinically significant differences for K, LDH, and TP analytes, they reported that they could be used with reference value transfer. 4 Orhan et al 20 observed that the K test in tubes with mechanical separators exceeded the TEa limit they set. Still, they suggested its use in the laboratory because of the benefits of using plasma.…”

Perbandingan Kadar Ureum Dan Kreatinin Antara Sampel Plasma Tabung Lithium Heparine Dan Serum Tabung Clot Activator

“…Fase praanalitik bertanggung jawab atas sumber utama variabilitas hasil laboratorium. 1-3 Jenis tabung penampung darah memengaruhi kualitas sampel dan kadar analit yang diperiksa, 4 diantaranya kadar ureum dan kreatinin. Selain memengaruhi waktu tunggu hasil laboratorium, penggunaan tabung serum atau tabung dengan antikoagulan juga memiliki dampak yang signifikan disebabkan adanya perbedaan matriks dan zat yang menginterferensi.…”

“…3 Alternatif penambahan clot activator diharapkan berguna untuk waktu penyelesaian yang lebih singkat dan hasil yang akurat. 4 Penambahan clot activator mempercepat terjadinya koagulasi ketika darah ditampung pada tabung. 4,5 World Health Organization (WHO) menyatakan bahwa konstituen dalam plasma mencerminkan situasi patologis pasien lebih akurat dibandingkan dengan serum.…”

“…4 Penambahan clot activator mempercepat terjadinya koagulasi ketika darah ditampung pada tabung. 4,5 World Health Organization (WHO) menyatakan bahwa konstituen dalam plasma mencerminkan situasi patologis pasien lebih akurat dibandingkan dengan serum. 2 Penggunaan plasma mempunyai keuntungan penting bagi para profesional laboratorium.…”

“…5,9 Hal ini berdampak pada pengurangan waktu tunggu yang diperlukan (turn around time). 4,9 Waktu pemrosesan yang lebih singkat ini menjadikan sampel plasma lebih direkomendasikan pada uji biokimia darurat dibandingkan dengan serum. 4 Keunggulan lain sampel plasma dibandingkan serum adalah tidak adanya gangguan yang disebabkan oleh mikrofibrin pada alat kimia otomatis.…”