Comparison of Behavior, Morphology and Morphometry of Equine and Canine Adipose Derived Mesenchymal Stem Cells in Culture

Grzesiak, Jakub; Marycz, Krzysztof; Czogała, Joanna; Wrzeszcz, Karol; Nicpoń, Jakub

doi:10.4067/s0717-95022011000300059

Cited by 35 publications

(37 citation statements)

References 8 publications

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“…However, at P3, the nucleus length was 26.35µm (24h) and 25.22µm 120h). The nucleus lengths of the dog and horse AD-MSCs were 16.2µm, and 16.4µm, respectively (Grzesiak et al 2011). According to Grzesiak et al (2011), morphology and morphometry of equine and canine adipose derived mesenchymal stem cells (AD-MSCs) in culture tend to be very similar, however further studies of this topic are needed.…”

Section: Discussionmentioning

confidence: 99%

“…The nucleus lengths of the dog and horse AD-MSCs were 16.2µm, and 16.4µm, respectively (Grzesiak et al 2011). According to Grzesiak et al (2011), morphology and morphometry of equine and canine adipose derived mesenchymal stem cells (AD-MSCs) in culture tend to be very similar, however further studies of this topic are needed. Hoogduijn et al (2013) compared the morphology and size of mesenchymal stem cells (AD-MSCs) from two mammals of noticeably different body size (mouse and whale).…”

Section: Discussionmentioning

confidence: 99%

“…In response to this, studies have been developed to compare the morphology and size of mesenchymal stem cells mammalian species (Hoogduijn et al 2013, Grzesiak et al 2011. Morphology and morphometry of equine and canine adipose derived mesenchymal stem cells tend to be very similar, however further studies of this topic are needed (Grzesiak et al 2011).…”

Section: Introductionmentioning

confidence: 99%

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Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

et al. 2014

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RESUMO.-[Morfologia e morfometria das células-tronco mesenquimais isoladas de medula óssea de gato.] As células tronco mesenquimais são utilizadas na terapia de várias doenças na medicina humana e veterinária. As célu-las tronco foram isoladas da medula óssea de gato, entretanto, existem poucos dados referentes a morfologia e não existem informações sobre a morfometria das células tronco isoladas da medula óssea. Os objetivos do presente estudo foram o isolamento, avaliação do crescimento, potencial de diferenciação e caracterização morfológica e morfomé-trica das células mesenquimais de gato isoladas de medula óssea. A diferenciação in vitro foi realizada para confirmar a Mesenchymal stem cells (MSC) are increasingly being proposed as a therapeutic option for treatment of a variety of different diseases in human and veterinary medicine. Stem cells have been isolated from feline bone marrow, however, very few data exist about the morphology of these cells and no data were found about the morphometry of feline bone marrow-derived MSCs (BM-MSCs). The objectives of this study were the isolation, growth evaluation, differentiation potential and characterization of feline BM-MSCs by their morphological and morphometric characteristics. In vitro differentiation assays were conducted to confirm the multipotency of feline MSC, as assessed by their ability to differentiate into three cell lineages (osteoblasts, chondrocytes, and adipocytes). To evaluate morphological and morphometric characteristics the cells are maintained in culture. Cells were observed with light microscope, with association of dyes, and they were measured at 24, 48, 72 and 120h of culture (P1 and P3). The non-parametric ANOVA test for independent samples was performed and the means were compared by Tukey's test. On average, the number of mononuclear cells obtained was 12.29 (±6.05x10 6 ) cells/mL of bone marrow. Morphologically, BM-MSCs were long and fusiforms, and squamous with abundant cytoplasm. In the morphometric study of the cells, it was observed a significant increase in average length of cells during the first passage. The cell lengths were 106.97±38.16µm and 177.91±71.61µm, respectively, at first and third passages (24 h). The cell widths were 30.79±16.75 µm and 40.18±20.46µm, respectively, at first and third passages (24 h).The nucleus length of the feline BM-MSCs at P1 increased from 16.28µm (24h) to 21.29µm (120h). However, at P3, the nucleus length was 26.35µm (24h) and 25.22µm (120h). This information could be important for future application and use of feline BM-MSCs. ) células/mL de medula óssea. As células mesenquimais são longas e fusiformes, e escamosas com citoplasma abundante. No estudo morfométrico, observou-se aumento no comprimento médio das células durante a primeira passagem. As medidas de comprimento das células foram: 106,97±38,16µm e 177,91±71,61µm, respectivamente, na primeira e terceira passagens (24 horas). As medidas de largura das células foram: 30,79±16,75 µm e 40,18±20,46 µm, respectivamente, na primeira e terceir...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

et al. 2014

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“…The comparison group animals received 1 ml of betamethasone, applied intraarticularly, while horses from the control group were only limited in movement. Tissue collection was carried out at the base of the research horses' tails and was followed by immediate isolation of cells using the method described previously (Grzesiak et al 2011). In order to obtain a sufficient number of cells for the injections, AdMSCs were cultivated in vitro.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effect of adipose-derived mesenchymal stem cell injection in horses suffering from bone spavin

Nicpoń

Marycz

Grzesiak

2013

Polish Journal of Veterinary Sciences

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In this article we demonstrate the efficiency of autologous transplantations of adipose-derived mesenchymal stem cells for equine bone spavin treatment. Horses qualified to the study were divided into three groups: (i) research -treated with intra-articular injections of autologous stem cells, (ii) comparison treated with steroid drugs and (iii) control -untreated. All animals underwent comprehensive clinical examination before and after treatment. Our research confirms the long-term beneficial influence resulting from stem cell therapy in horse bone spavin treatment, in contrast to routine steroid usage.

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“…Owners of the animals provided proper agreement for the procedure. All stages of the procedure were performed as previously described [16,17] . Fat tissue biopsies (2 g) were washed in Hank's Balanced Salt Solution (HBSS) from tissue contaminations and digested in type I collagenase (5 mg/ml).…”

Section: Isolation Of Admscsmentioning

confidence: 99%

The Morphology and Proliferation Rate of Canine and Equine Adipose Derived Mesenchymal Stem Cells Cultured with Flunixin Meglumine-in vitro

Marycz¹,

Toker²,

Śmieszek³

et al. 2014

Kafkas Univ Vet Fak Derg

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The regenerative medicine in animals is a rapidly growing field, especially when therapies with stem cells are applied. Currently, stem cells are used for treatment of orthopedic diseases occurring both in small and large animals. Non-steroidal anti-inflammatory drugs (NSAIDs) are routinely used and are often accompanied with adipose derived mesenchymal stem cells (ADMSCs) therapy. Therefore, it is reasonable to monitor morphology of cells and the proliferation status of canine and equine ADMSCs cultured with NSAIDs. We focused on the analysis of the above mentioned parameters. Morphology of investigated cells was monitored using an epifluorescence microscope and scanning electron microscope (SEM). Moreover, SEM analysis was carried out for determination of microvesicles secretion. Proliferation activity of AdMSCs was evaluated with a resazurin-based test. Our research showed that the lowest concentration of Flunixin meglumine (0.01 mg/ml) had a stimulating effect on canine AdMSCs proliferation, while the same concentration significantly slowed down equine stem cell growth. Interestingly, the 0.01 mg/ml concentration of Flunixin meglumine did not effect morphology of the investigated stem cells population. Thus, results obtained from multilevel research allow us to conclude that the lowest concentration of Flunixin meglumine may be accompanied with canine adipose derived mesenchymal stem cells in orthopedic treatment. Keywords ÖzetHayvanlarda özellikle kök hücrelerinin kullanıldığı rejeneratif (yenilenebilir) tıp hızla gelişim gösteren bir alandır. Günümüzde hem büyük hem de küçük hayvanların ortopedik hastalıklarında kök hücre tedavisinden faydalanılmaktadır. Klinik pratikte rutin olarak kullanılan non-steroidal anti-inflamatuar ilaçlar, adipoz kökenli mezenşimal kök hücresi tedavisine de dahil edilmektedir. Bu çalışmada köpek ve atlardan elde edilmiş adipoz kökenli mezenşimal kök hücrelerinin, yaygın kullanılan bir non-steroidal anti-inflamatuar ilaç (flunixin meglumine) ile kültüre edilmesinin, hücre morfolojisi ve proliferasyonu üzerine etkileri araştırıldı. Hücrelerin morfolojik takibinde epifuloresans mikroskop ve taramalı elektron mikroskobu (SEM) kullanıldı. Hücrelerin mikrovezikül sekresyonları da SEM aracılığıyla izlendi. Adipoz kökenli mezenşimal kök hücrelerinin proliferasyon aktiviteleri resazurin-temelli bir test yardımıyla değerlendirildi. Araştırma sonuçlarına göre fluniksin meglumin'in en düşük konsantrasyonlarının (0.01 mg/ml) köpeklerden elde edilen adipoz kökenli mezenşimal kök hücrelerinde proliferasyonu uyardığı; ancak aynı konsantrasyonun at kökenli hücrelerde proliferasyonu belirgin düzeyde yavaşlattığı gözlendi. Enteresan bir sonuç olarak 0.01 mg/ml konsantrasyondaki flunixin meglumine'in, incelenen kök hücre populasyonunun morfolojilerine etki etmediği belirlendi. Çok aşamalı bu araştırmadan elde edilen bulgular ışığında, köpeklerden elde edilen adipoz kökenli mezenşimal kök hücrelerinin ortopedik tedavilerde kullanımı sırasında, flunixin meglumine'in en düşük konsantrasyonlarda kullan...

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Comparison of Behavior, Morphology and Morphometry of Equine and Canine Adipose Derived Mesenchymal Stem Cells in Culture

Cited by 35 publications

References 8 publications

Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

Morphology and morphometry of feline bone marrow-derived mesenchymal stem cells in culture

Therapeutic effect of adipose-derived mesenchymal stem cell injection in horses suffering from bone spavin

The Morphology and Proliferation Rate of Canine and Equine Adipose Derived Mesenchymal Stem Cells Cultured with Flunixin Meglumine-in vitro

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