Comparison of bioactive components and pharmacological activities of ophiopogon japonicas extracts from different geographical origins

Zhao, Min; Xu, Wanfeng; Shen, Hao; Shen, Pei-qiang; Zhang, Jun; Wang, Dandan; Xu, Han; Wang, Hong; Yan, Tingting; Wang, Lin; Hao, Haiping; Wang, Guangji; Cao, Lijuan

doi:10.1016/j.jpba.2017.02.013

Cited by 33 publications

(26 citation statements)

References 26 publications

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“…It was also demonstrated that the Zhejiang O. japonicus extract had a stronger antioxidant and anti-inflammatory capacity than the Sichuan O. japonicus extract. Interestingly, both O. japonicus root extracts exhibited cytotoxicity with different selectivity on cancer cell lines in vitro [24]. Ge also reported that O. japonicus from different producing areas possessed different properties with cytotoxicity and drug-metabolising enzyme induction [25].…”

Section: Introductionmentioning

confidence: 95%

“…Similar to many other medicinal plants, O. japonicus roots are currently grown in several locations across China. Previous studies have shown that the quality of O. japonicus roots is influenced by different factors such as species [22] and growth location [23,24]. Among these factors, the geographical origin of O. japonicus has been proven to produce most of the variation in its clinical activity.…”

Section: Introductionmentioning

confidence: 99%

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Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms

Chen

Gođevac

et al. 2019

Planta Med

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Ideally, metabolomics should deal with all the metabolites that are found within cells and biological systems. The most common technologies for metabolomics include mass spectrometry, and in most cases, hyphenated to chromatographic separations (liquid chromatography- or gas chromatography-mass spectrometry) and nuclear magnetic resonance spectroscopy. However, limitations such as low sensitivity and highly congested spectra in nuclear magnetic resonance spectroscopy and relatively low signal reproducibility in mass spectrometry impede the progression of these techniques from being universal metabolomics tools. These disadvantages are more notorious in studies of certain plant secondary metabolites, such as saponins, which are difficult to analyse, but have a great biological importance in organisms. In this study, high-performance thin-layer chromatography was used as a supplementary tool for metabolomics. A method consisting of coupling 1H nuclear magnetic resonance spectroscopy and high-performance thin-layer chromatography was applied to distinguish between Ophiopogon japonicus roots that were collected from two growth locations and were of different ages. The results allowed the root samples from the two growth locations to be clearly distinguished. The difficulties encountered in the identification of the marker compounds by 1H nuclear magnetic resonance spectroscopy was overcome using high-performance thin-layer chromatography to separate and isolate the compounds. The saponins, ophiojaponin C or ophiopogonin D, were found to be marker metabolites in the root samples and proved to be greatly influenced by plant growth location, but barely by age variation. The procedure used in this study is fully described with the purpose of making a valuable contribution to the quality control of saponin-rich herbal drugs using high-performance thin-layer chromatography as a supplementary analytical tool for metabolomics research.

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Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms

Chen

Gođevac

et al. 2019

Planta Med

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“…As bajitian is processed from M. officinalis roots, whereas our specimen for the transcriptome sequencing was from its leaves, although the chances are small, it is possible that the genes responsible for these chemical compounds may be specific to the roots [35]. Alternatively, the differences in reported gene synthases could be caused by phenotypic changes, whereby a comprehensive comparison of bioactive components and pharmacological activities between different geographical origins could perhaps be able to link components to the related genes in this species [36].…”

Section: Categories and Annotations Of Unigenes Containing Ssrsmentioning

confidence: 99%

Characterization and novel Est-SSR marker development of an important Chinese medicinal plant, Morinda officinalis How (Rubiaceae)

Liao

Lee

Meng

et al. 2019

Biotechnology & Biotechnological Equipment

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Morinda officinalis How (Rubiaceae) is well known as one of the four most popular medicinal plants in the southern region of China. In order to assess its genetic diversity and assist the development of its molecular assisted-selection breeding in China, we constructed the transcriptome database of M. officinalis using fresh leaf material and developed novel expressed sequence tag-simple sequence repeat (EST-SSR) markers for this important medicinal plant species. Our study identified 8064 potential EST-SSRs from 6668 unigenes. For perfect repeat motifs, the mononucleotide repeats were most abundant (4221; 52.34%), followed by the trinucleotide repeats (1588; 19.69%), and the dinucleotide ones (1040; 12.90%). The seven most commonly occurring repeat motifs were A/T (4661), AG/CT (588), AAG/CTT (408), AC/GT (353), ATC/ATG (338), AT/AT (296) and AGG/CCT (277). Of a total of 5605 SSR primer sets that were successfully designed de novo, 100 were selected for validation analysis. There, 24 primer sets were polymorphic across the 96 M. officinalis individuals collected from four different populations in Guangdong province. Based on cross-species transferability test, 19 and 22 primer sets produced successful amplification in M. umbellata subsp. obovata and M. parvifolia, respectively. These novel EST-SSR markers with high cross-species transferability between M. officinalis and its closely-related species could serve as aids to future studies in genetic diversity, conservation, assisted-selection breeding and genes associated with the medical uses of the genus.

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“…Bygone investigations have discovered that the Chinese herb Mai-Dong contains several classes of ingredients, including homoisoflavonoids, steroidal saponins and polysaccharides (Chen et al, 2016a;Zhao et al, 2017;Fang et al, 2018;Chen et al, 2019;Tan et al, 2019;Tian et al, 2019;Zhan et al, 2019). Among all reported ingredients, the homoisoflavonoids (such as methylophiopogonanone A and its analougs) have been identified as the principal bioactive compounds and marker compounds of Mai-Dong, which have been found with broad biological activities including anti-cancer, anti-inflammatory and anti-bacterial activities (Zhu et al, 2004;Lin et al, 2010;Li et al, 2012;Wang et al, 2017;Wang et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Reversible and Irreversible Inhibition of Cytochrome P450 Enzymes by Methylophiopogonanone A

Mao

Zhang

et al. 2021

Drug Metab Dispos

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Methylophiopogonanone A (MOA), an abundant homoisoflavonoid bearing a methylenedioxyphenyl (MDP) moiety, is one of the major consitituents in the Chinese herb Ophiopogon japonicas. This work aims to assess the inhibitory potentials of MOA against cytochrome P450 enzymes, as well as to decipher the molecular mechanisms for CYP inhibition by MOA. The results showed that MOA concentration-dependently inhibited CYPs1A, 2C8, 2C9, 2C19 and 3A in human liver microsomes (HLMs) in a reversible way, with IC 50 values varying from 1.06 μM to 3.43 μM. By contrast, MOA time-, concentrationand NADPH-dependently inhibited CYP2D6 and CYP2E1, along with K I and k inact values of 207 µM and 0.07 min -1 for CYP2D6, as well as 20.9 µM and 0.03 min -1 for CYP2E1. Further investigations demonstrated that a quinone metabolite of MOA could be trapped by GSH in a HLM incubation system, while CYPs2D6, 1A2 and 2E1 were the major contributors to catalyze the metabolic activation of MOA to the corresponding O-qunione intermediate.Additionally, the potential risks of herb-drug interactions triggered by MOA or MOA-related products were also predicted. Collectively, our findings verify that MOA is a reversible inhibitor of CYPs1A, 2C8, 2C9, 2C19 and 3A but acts as an inactivator of CYP2D6 and CYP2E1.

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Comparison of bioactive components and pharmacological activities of ophiopogon japonicas extracts from different geographical origins

Cited by 33 publications

References 26 publications

Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms

Metabolic Profiling of Saponin-Rich Ophiopogon japonicus Roots Based on 1H NMR and HPTLC Platforms

Characterization and novel Est-SSR marker development of an important Chinese medicinal plant, Morinda officinalis How (Rubiaceae)

Reversible and Irreversible Inhibition of Cytochrome P450 Enzymes by Methylophiopogonanone A

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