Comparison of biochemical and histological methods of estimating fat content of liver of dairy cows

Gaál, T.; Reid, I. M.; Collins, Robert A.; Roberts, C. J.; Pike, B.V.

doi:10.1016/s0034-5288(18)32268-9

Cited by 54 publications

(43 citation statements)

References 11 publications

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“…The ORO stain was more accurate, because ORO identifies mainly TAG and less phospholipids and cholesterol [15]. A steriological analysis of fatty livers revealed a highly significant correlation with the hepatic TAG content in cattle [8]. Also Collins et al revealed a high correlation between TAG content and volume fraction of fat droplets determined stereologically [23].…”

Section: Discussionmentioning

confidence: 99%

“…In addition, the assessment of a biopsy is only successful in diffuse hepatopathies. A percutaneous liver biopsy is always taken from the right lobe and represents 0.002 to 0.1% of the whole liver parenchyma, depending on the size of the biopsy needle [8]. Fatty liver in cattle is reported to be diffuse [8].…”

Section: Introductionmentioning

confidence: 99%

“…A percutaneous liver biopsy is always taken from the right lobe and represents 0.002 to 0.1% of the whole liver parenchyma, depending on the size of the biopsy needle [8]. Fatty liver in cattle is reported to be diffuse [8]. However, in beef bulls concentrations of cholesterol and phospholipids were higher in the intermediate lobe, compared to the right and left lobes [9].…”

Section: Introductionmentioning

confidence: 99%

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Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver

et al. 2017

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BackgroundThe assessment of a liver biopsy remains the gold standard for diagnosing and staging fatty liver in dairy cows, which is often necessary for diagnostic and research purposes. Accuracy of the diagnosis relays on the quality of the biopsy, the assumed representativeness of a small tissue sample for a disease process throughout the liver and accurate human evaluation of histologic specimens. The objective of the present study was to assess the distribution of triacylglycerol (TAG) infiltration throughout the parenchyma of livers with different degrees of fatty liver in dairy cows. In addition, histopathological scores from the corresponding specimens were compared to a quantitative measurement of TAG, as well as the agreement between two observers.MethodsThirty livers with different degrees of lipid infiltration were selected and 10 different locations throughout the liver were assessed. The TAG content was measured enzymatically, calculated in % or mg/g wet weight, and assigned to a scoring system. Corresponding tissue specimens were stained with hematoxylin-eosin (H&E) and Oil red O (ORO) for histopathological evaluation, using a scoring system.ResultsThe difference in TAG content between any locations was less than 2%. Based on the scoring system the TAG concentration was even distributed in 79.3% of the livers. Based on kappa statistics the agreement between two pathologists and staining technique in scoring histological specimens was moderate to fair.ConclusionsOverall the distribution of TAG throughout the liver and the accuracy of human evaluation of liver biopsies may lead to acceptable diagnoses for clinical purposes. Within the liver lobules a common pattern of lipid distribution depending on severity could be observed. For the staging of lipid infiltration for research projects, some degree of variation needs to be considered.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver

et al. 2017

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“…Sections (12 μ m) were taken from cryostat, and these were stained with Sudan Black B stain [17]. Fat rate in hepatocytes was determined with the method reported by Gaal et al [18]. …”

Section: Methodsmentioning

confidence: 99%

Effects of Aflatoxin on Liver and Protective Effectiveness of Esterified Glucomannan in Merino Rams

Çolakoğlu

Dönmez

2012

The Scientific World Journal

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The effects of total aflatoxin (AF) given orally on liver in Merino rams were studied. In addition, this study was conducted in order to evaluate the efficacy of an esterified glucomannan (EG) for protection against aflatoxicosis. One-year-old 32 Merino rams were divided into four equal groups. The control group (C) was fed with the commercial feed. The AF group was fed with commercial feed plus 250 μg/day of total AF. The EG group was fed with commercial feed plus 2 g/day of EG. The AF + EG group was fed with commercial feed plus 250 μg/day of total AF and 2 g/day of EG. After feeding period, tissue samples were taken from the liver in order to perform histological analyses. Vacuolar degeneration with small and large droplets and hydropic degeneration in hepatocytes were observed in the AF group. The ceroid pigmentation was observed in macrophages in groups or one by one. It was observed that the fat rate in hepatocytes was 2.6% in the C group, 35.5% in the AF group, 2.9% in the EG group, and 9.6% in the AF + EG group. In conclusion, the adverse effects caused by aflatoxicosis on the liver could be ameliorated by adding EG to the ration.

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“…Cows with less than 50 mg of triglyceride per gram of wet liver weight were considered to be in the normal group, those with 50-100 mg in the moderate fatty liver group, and those with more than 100 mg were considered as severely fatty in agreement with the classification proposed by Gaal et cK (22). The animals were divided into experimental groups according to the triglyceride content of the fatty liver.…”

Section: Methodsmentioning

confidence: 99%

Serum apolipoproteins B and A‐I and naturally occurring fatty liver in dairy cows

Marcos¹,

Mazur²,

Cardot³

et al. 1990

Lipids

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Serum lipids and apolipoprotein (apo) B and A-I concentrations were determined in 164 dairy cows which had undergone liver biopsy in early lactation. The animals were divided into groups according to fatty liver severity on the basis of hepatic triglyceride content. The serum free fatty acid (FFA) concentration was higher in cows that developed fatty livers than in normal cows, and it correlated highly with liver triglycerides. Serum total cholesterol and triglyceride levels did not correlate with hepatic triglycerides. Both apo B and apo A-I levels were significantly decreased in fatty liver cows. In particular, apo B levels showed a strongly negative correlation with liver triglycerides. The present results suggest that hepatic apolipoprotein synthesis is impeded in fatty liver cows.

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Comparison of biochemical and histological methods of estimating fat content of liver of dairy cows

Cited by 54 publications

References 11 publications

Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver

Variation in fat content between liver lobes and comparison with histopathological scores in dairy cows with fatty liver

Effects of Aflatoxin on Liver and Protective Effectiveness of Esterified Glucomannan in Merino Rams

Serum apolipoproteins B and A‐I and naturally occurring fatty liver in dairy cows

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