Comparison of biogenic and chemical sulfur as electron donors for autotrophic denitrification in sulfur-fed membrane bioreactor (SMBR)

Uçar, Deniz; Yılmaz, Tülay; Capua, Francesco Di; Esposito, Giovanni; Şahinkaya, Erkan

doi:10.1016/j.biortech.2019.122574

Cited by 69 publications

(13 citation statements)

References 38 publications

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“…Biogenic S 0 , with a structure made of orthorhombic S 0 crystals, is known to be hydrophilic, stabilized in water by long-chain polymers (mainly proteins), making it more bioavailable for SOB . In comparison, chemically synthesized S 0 is hydrophobic, with very low solubility, resulting in lower denitrification rates . SOB growth on biogenic S 0 was found to be 1.7-fold faster than on chemically synthesized S 0 , but it was still much lower than that on thiosulfate.…”

Section: Resultsmentioning

confidence: 97%

Assessing Intermediate Formation and Electron Competition during Thiosulfate-Driven Denitrification: An Experimental and Modeling Study

Yang

Calleja

Liu

et al. 2022

Environ. Sci. Technol.

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There is increasing interest in thiosulfate-driven denitrification for low C/N wastewater treatment, but the denitrification performance varies with the thiosulfate oxidation pathways. Models have been developed to predict the products of denitrification, but few consider thiosulfate reduction to elemental sulfur (S0), an undesirable reaction that can intensify electron competition with denitrifying enzymes. In this study, the model using indirect coupling of electrons (ICE) was developed to predict S0 formation and electron competition during thiosulfate-driven denitrification. Kinetic data were obtained from sulfur-oxidizing bacteria (SOB) dominated by the branched pathway and were used to calibrate and validate the model. Electron competition was investigated under different operating conditions. Modeling results reveal that electrons produced in the first step of thiosulfate oxidation typically prioritize thiosulfate reduction, then nitrate reduction, and finally nitrite reduction. However, the electron consumption rate for S0 formation decreases sharply with the decline of thiosulfate concentration. Thus, a continuous feeding strategy was effective in alleviating the competition between thiosulfate reduction and denitrifying enzymes. Electron competition leads to nitrite accumulation, which could be a reliable substrate for anammox. The model was further evaluated with anammox integration. Results suggested that the branched pathway and continuous supply of thiosulfate are favorable to create a symbiotic relationship between SOB and anammox.

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Section: Resultsmentioning

confidence: 97%

Assessing Intermediate Formation and Electron Competition during Thiosulfate-Driven Denitrification: An Experimental and Modeling Study

Yang

Calleja

Liu

et al. 2022

Environ. Sci. Technol.

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“…NGS analysis further confirmed that the granules community was dominated by heterotrophic, nitrifying and denitrifying genera suggesting that shortcut nitrification-denitrification instead of PN/A process was established since the first weeks of operation. Chryseobacterium (genera containing species both capable of heterotrophic nitrification and aerobic denitrification [ 54 , 55 ]) was the most common genera (7.7–27.2%), while other dominant genera were Hylemonella (7.3–13.9%, denitrifiers [ 56 ]), followed by Nitrosomonas (9.9–14.0%, mainly nitrifiers [ 57 ]), Parvibaculum (7.0–11.7%, aerobic hydrocarbon-degrading bacteria and denitrifiers [ 58 , 59 ]), Rubrivivax (3.3–15.7%, involved in multiple biogeochemical and aromatic transformations, also denitrification, due to a wide metabolic capacity [ 58 , 60 ]) and other Comamonadaceae (4.3–8.9%, aromatic degraders and denitrifiers [ 61 ]), Chitinophagaceae (3.6–10.9%), other Saphrospirales (0.1–9.4%), Sphingopyxis (2.2–4.7%), other Burkholderiales (2.1–4.3%), Bdellovibrio (0.7–2.9%), Diaphorobacter (1.2–2.2%, both heterotrophic nitrifiers and denitrifiers [ 62 ]), Rhodanobacter (1.0–1.6%), the families of Alcaliganaceae (0.7–1.7%) and Xanthomonadaceae (0.8–1.1%), Clostridium (0.3–1.3%, biopolymers degraders able of N fixation [ 63 , 64 ]), Thermomonas (0.6–1.0%, denitrifiers [ 65 , 66 ]), Pseudomonas (0.0–1.4%, capable of both heterotrophic nitrification and/or aerobic denitrification [ 67 ]), and the family of Bradyrhizobiaceae (0.2–1.0%, N fixation [ 68 ]), other bacteria that singularly accounted for less than 1% of the total abundance were 7.6–14.3%.…”

Section: Resultsmentioning

confidence: 99%

Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater

Clagnan

Brusetti

Pioli

et al. 2021

Heliyon

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Implementation of onsite bioremediation technologies is essential for textile industries due to rising concerns in terms of water resources and quality. Partial nitritation-anaerobic ammonium oxidation (PN/A) processes emerged as a valid, but unexplored, solution. In this study, the performance of a PN/A pilot-scale (9 m 3 ) sequencing batch reactor treating digital textile printing wastewater (10–40 m 3 d −1 ) was monitored by computing nitrogen (N) removal rate and efficiencies. Moreover, the structure of the bacterial community was assessed by next generation sequencing and quantitative polymerase chain reaction (qPCR) analyses of several genes, which are involved in the N cycle. Although anaerobic ammonium oxidation activity was inhibited and denitrification occurred, N removal rate increased from 16 to 61 mg N g VSS −1 d −1 reaching satisfactory removal efficiency (up to 70%). Ammonium (18–70 mg L −1 ) and nitrite (16–82 mg L −1 ) were detected in the effluent demonstrating an unbalance between the aerobic and anaerobic ammonia oxidation activity, while constant organic N was attributed to recalcitrant azo dyes. Ratio between nitrification and anammox genes remained stable reflecting a constant ammonia oxidation activity. A prevalence of ammonium oxidizing bacteria and denitrifiers suggested the presence of alternative pathways. PN/A resulted a promising cost-effective alternative for textile wastewater N treatment as shown by the technical-economic assessment. However, operational conditions and design need further tailoring to promote the activity of the anammox bacteria.

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“…SOB is a group of microorganisms which oxidize the sulfide, elemental sulfur, thiosulfite and sulfite to sulfate or intermediates. In Y2 sediments, SOB of Gallionella, Sulfuriferula and Thermomonas was observed, and the abundance of Gallionella and Sulfuriferula was very low; Thermomonas is a strict anaerobic bacteria that could drives denitrification coupling with the oxidation of reduced inorganic sulfur compounds (He et al 2017;Yavuz et al 2007;Ucar et al 2020). Gallionella, Sulfuriferula and Ferruginibacter were observed in YO sediments.…”

Section: Vertical Distribution Of Microbial Diversitymentioning

confidence: 99%

Diverse Biotransformation of Sulfur in Antarctic Lake Sediments

Song

Huang

Chen

et al. 2021

Preprint

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Microbial communities, sulfur isotope of sulfides (δ34SAVS and δ34SCRS) and sulfur and oxygen isotopes of sulfate (δ34SSO4 and δ18OSO4) in sediments were analyzed to study the biotransformation of sulfur in a penguin-affected lake Y2 and a pristine YO from Fildes Peninsula, Antarctic Peninsula. The microbial communities in Y2 were mainly associated with penguin activities, while those in YO were limited by nutrients. The much enriched δ34SSO4 recorded at depth of 30, 41 and 52 cm in Y2 indicates very strong sulfate reduction therein. The sulfur-degrading bacteria Pseudomonas in 0–23 cm of Y2 was 3.5 times as abundant as that of sulfur oxidizing bacteria (SOB), indicating remarkable remineralization of organic sulfur. While abundant SOB and 34S-depleted sulfate indicate considerable sulfur oxidation in 34–56 cm layer in Y2. In YO sediments, the highest abundance of Desulfotalea and the most enriched δ34SSO4 (35.2‰) and δ34SCRS (2.5‰) indicate strongest sulfate reduction in 28 cm layer. High abundance of Pseudomonas indicates active remineralization of organic sulfur in 3–5 cm layer in YO. While the medium δ34SSO4 and considerable abundance of SOB and SRB indicate concurrence of sulfur oxidation and sulfate reduction in other layers in YO. Our results show that high level of organic matter inputs from penguin populations support the diverse microbial community and biotransformation of sulfur in freshwater ecosystems in Antarctica.

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Comparison of biogenic and chemical sulfur as electron donors for autotrophic denitrification in sulfur-fed membrane bioreactor (SMBR)

Cited by 69 publications

References 38 publications

Assessing Intermediate Formation and Electron Competition during Thiosulfate-Driven Denitrification: An Experimental and Modeling Study

Assessing Intermediate Formation and Electron Competition during Thiosulfate-Driven Denitrification: An Experimental and Modeling Study

Microbial community and performance of a partial nitritation/anammox sequencing batch reactor treating textile wastewater

Diverse Biotransformation of Sulfur in Antarctic Lake Sediments

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