Comparison of biological measurement and physical estimates of space radiation in the International Space Station

Yoshida, Kayo; Hada, Megumi; Kizu, Akane; Kitada, Kohei; Eguchi-Kasai, Kiyomi; Kokubo, Toshiaki; Teramura, Takeshi; Yano, Sachiko; Suzuki, Hiromi; Watanabe, Hidenobu; Kondoh, Gen; Nagamatsu, Aiko; Saganti, Premkumar B.; Cucinotta, Francis A.; Morita, Takashi

doi:10.1016/j.heliyon.2022.e10266

Cited by 10 publications

(7 citation statements)

References 34 publications

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“…The quality factor of the ISS was estimated to be about 1.48 on the ISS. This physically estimated value was similar to the relative biological effectiveness (RBE) of 1.54, which was established by the biological measurement of chromosome aberration using frozen mouse ES cells in the MELFI freezer on the ISS [ 19 ].…”

Section: Introductionmentioning

confidence: 63%

“…For space experiments, we used wild-type mouse ES cells. Preparation of mouse ES cells lacking the histone H2AX gene with 129 Sv and C57BL/6 heterozygous background was performed as described before [ 19 ].…”

Section: Methodsmentioning

confidence: 99%

“…Frozen mouse ES cells were thawed at 37 °C. After adding the warmed culture medium [ 19 ], the cells were inoculated on an 8-hole plastic dish and cultured for the experimentally determined time. The pre-culture cell samples and time-indicated samples were taken separately after rubbing with scrapers.…”

Section: Methodsmentioning

confidence: 99%

“…The dose-equivalent rates, indicating the biological effect that is calculated from a physical dose measurement, are about 0.5–0.62 mSv/day on the International Space Station (ISS) [ 11 , 12 ], 1.15 mSv/day on the surface of the moon of the Earth [ 13 , 14 ], 0.5 mSv/day on the surface of Mars [ 15 ], and 1.84 mSv/day during the flight from the Earth to Mars [ 16 , 17 , 18 ]. In our “Stem Cells” experiment, mouse ES cells were frozen and kept in MELFI on the ISS for 1584 days to quantitate the biological effect of space radiation [ 18 , 19 ]. The physical doses were measured by a passive dosimeter for life science experiments in space (PADLES), and it was revealed that the absorbed dose rate of space radiation in MELFI was 0.36 mGy/day, and the dose-equivalent rate was 0.53 mSv/day, as shown in Figure 1 [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…In our “Stem Cells” experiment, mouse ES cells were frozen and kept in MELFI on the ISS for 1584 days to quantitate the biological effect of space radiation [ 18 , 19 ]. The physical doses were measured by a passive dosimeter for life science experiments in space (PADLES), and it was revealed that the absorbed dose rate of space radiation in MELFI was 0.36 mGy/day, and the dose-equivalent rate was 0.53 mSv/day, as shown in Figure 1 [ 19 ]. About 4.1% of particles had more than 10 keV/μm of LET (linear energy transfer), but they contributed 35.3% of total dose-equivalent.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis by RNA Sequencing of Mouse Embryonic Stem Cells Stocked on International Space Station for 1584 Days in Frozen State after Culture on the Ground

Yoshida,

Hada,

Hayashi

et al. 2024

IJMS

Self Cite

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As a space project, in “Stem Cells” by the Japan Aerospace Exploration Agency (JAXA), frozen mouse ES cells were stored on the International Space Station (ISS) in the Minus Eighty Degree Laboratory Freezer for ISS (MELFI) for 1584 days. After taking these cells back to the ground, the cells were thawed and cultured, and their gene expressions were comprehensively analyzed using RNA sequencing in order to elucidate the early response of the cells to long-time exposure to space radiation consisting of various ionized particles. The comparisons of gene expression involved in double-stranded break (DSB) repair were examined. The expressions of most of the genes that were involved in homologous recombination (HR) and non-homologous end joining (NHEJ) were not significantly changed between the ISS-stocked cells and ground-stocked control cells. However, the transcription of Trp53inp1 (tumor protein 53 induced nuclear protein-1), Cdkn1a (p21), and Mdm2 genes increased in ISS-stocked cells as well as Fe ion-irradiated cells compared to control cells. This suggests that accumulated DNA damage caused by space radiation exposure would activate these genes, which are involved in cell cycle arrest for repair and apoptosis in a p53-dependent or -independent manner, in order to prevent cells with damaged genomes from proliferating and forming tumors.

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Section: Introductionmentioning

confidence: 63%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Transcriptome Analysis by RNA Sequencing of Mouse Embryonic Stem Cells Stocked on International Space Station for 1584 Days in Frozen State after Culture on the Ground

Yoshida,

Hada,

Hayashi

et al. 2024

IJMS

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Cosmic‐Ray Radiation Effects on Ibuprofen Tablet Formulation Inside and Outside of the International Space Station

Tran,

Spooner,

Geoghehan

et al. 2024

Adv Healthcare Materials

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In extreme environments people will have different needs for medicine(s), making it crucial to understand how such environments affect drug efficacy. Ibuprofen, commonly used in tablet formulation on Earth, could fail in space despite standard pharmaceutical packaging. We introduce the concept of ‘space medicines’, where solid‐dosage forms protect the pharmaceutical from accelerated degradation in spaceflight. We simulate dose(s) in International Space Station (ISS) through radionuclide and photon experiments, and establish the impact of alpha, beta and gamma rays. We demonstrate that tablet formulation protects from impact of alpha and beta rays; however, gamma rays decompose ibuprofen even when ‘masked’. We systematically analyse 19 tablet compositions inside and outside the ISS to determine the effect of compositional changes in the tablet matrix. We confirm that the iron oxide‐shielded tablets show minimal degradation (〈10%) inside the ISS, compared to moderate reductions (〉10%) for other formulations, with one exception. The tablets exhibited significantly greater ibuprofen degradation (〉 30‐50%) outside ISS, due to harsh conditions. Significantly, we found that flavour have shielding potential by scavenging free radicals. We conclude that ibuprofen efficacy is adversely affected in space, and these effects are expected to worsen on missions to deeper space destinations.

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Integrating evolutionarily conserved mechanism of response to radiation for exploring novel Caenorhabditis elegans radiation-responsive genes for estimation of radiation dose associated with spaceflight

Zhao,

Li,

Huang

et al. 2024

Chemosphere

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Comparison of biological measurement and physical estimates of space radiation in the International Space Station

Cited by 10 publications

References 34 publications

Transcriptome Analysis by RNA Sequencing of Mouse Embryonic Stem Cells Stocked on International Space Station for 1584 Days in Frozen State after Culture on the Ground

Transcriptome Analysis by RNA Sequencing of Mouse Embryonic Stem Cells Stocked on International Space Station for 1584 Days in Frozen State after Culture on the Ground

Cosmic‐Ray Radiation Effects on Ibuprofen Tablet Formulation Inside and Outside of the International Space Station

Integrating evolutionarily conserved mechanism of response to radiation for exploring novel Caenorhabditis elegans radiation-responsive genes for estimation of radiation dose associated with spaceflight

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