Comparison of biological processes induced in HepG2 cells by tert-butyl hydroperoxide (t-BHP) and hydroperoxide (H2O2): The influence of carvacrol

In vitro genotoxicity testing of carvacrol and thymol using the micronucleus and mouse lymphoma assays

“…On the other hand, although a dose-dependent response was reported in human lymphocytes for thymol using the comet assay, only the higher concentrations of this compound (above 0.1-0.2 mM) induced DNA damage [21][22]. These results contrast with those reported by Slamenova et al [38] who reported that thymol significantly increased the frequency of sister-chromatid exchanges (SCE) and induced structural CA and MN in human lymphocytes at all of the concentrations tested (25-100 g/mL, equivalent to 17-66.5 M). These contradictory results are related to the differences between the selected cells types, with human lymphocytes being more sensitive than established cell-lines, including mouse lymphoma cells (used in our present study).…”

“…The absence of genotoxicity observed for thymol in the present work could be attributed to its extensively proven antioxidant properties [21,24,38]. As a phenolic compound, thymol has high potential to scavenge oxidative radicals by donating protons from its phenolic hydroxyl groups [38]. This fact has already been described in several studies, which reported the antigenotoxic or protective effects of thymol in several experimental models exposed to DNA-damaging agents [21][22][23]39].…”

“…These contradictory results are related to the differences between the selected cells types, with human lymphocytes being more sensitive than established cell-lines, including mouse lymphoma cells (used in our present study). The absence of genotoxicity observed for thymol in the present work could be attributed to its extensively proven antioxidant properties [21,24,38]. As a phenolic compound, thymol has high potential to scavenge oxidative radicals by donating protons from its phenolic hydroxyl groups [38].…”

An assessment of techniques to manipulate oxidative stress in animals

“…; Slamenova et al . ). The cellular effects of t BHP have been found to be comparable to those of H 2 O 2 exposure, though t BHP has the logistical advantage of remaining chemically stable until administered to cells or tissue (Spector et al .…”

Mutagenicity, genotoxicity and gene expression of Rad51C, Xiap, P53 and Nrf2 induced by antimalarial extracts of plants collected from the middle Vaupés region, Colombia

“…Por otro lado, la sobreexpresión de P53, Rad51C y Xiap podría explicarse por la incapacidad celular para reducir los niveles de estas especies, los cuales interactuarían con el ADN causando rupturas en la doble cadena (43). En tal contexto, el gen P53 detendría el ciclo celular para permitir la activación de los sistemas de reparación, así como el gen Rad51C, molécula clave en la reparación del ciclo celular de células mitóticas por recombinación homóloga en el punto de control S/G (34).…”