Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

Safitri, Erma; Hariadi, Mas’ud

doi:10.14202/vetworld.2019.916-924

Cited by 11 publications

(26 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Pada kelompok kontrol negatif (K-), tikus tidak dipuasakan dan tidak diberi madu, sedangkan pada kelompok kontrol positif (K+), tikus dipuasakan selama 5 hari dan tidak diberi madu. Pada kelompok P1, dan P2 tikus dipuasakan selama 5 hari dan masing-masing diberi madu 30% dan 50% dalam air minum secara ad libitum selama 10 hari (Safitri et al, 2019). Madu yang digunakan dalam penelitian ini adalah madu monoflora yaitu madu murni kaliandra.…”

Section: Perlakuan Hewan Cobaunclassified

POTENSI MADU PADA PENINGKATAN JUMLAH SEL SPERMATOGENIK TIKUS (Rattus norvegicus) YANG KEKURANGAN NUTRISI

Listyorini

Hernawati²,

Suprayogi³

2021

Ovz

View full text Add to dashboard Cite

This study aimed to determine honey's potential in increasing the number of spermatogenic cells (spermatogonium, spermatocyte and spermatid) of rats (Rattus norvegicus) with nutritional deficiencies. Twenty four male rats (Rattus norvegicus) were randomly divided into four groups. Negative control (K-) mice was not fasted nor given honey. Positive control (K+) mice was fasted for 5 days without the administration of honey. The treatment group rats were fasted for 5 days followed by administration of honey 30% (P1) and 50% (P2) in drinking water for 10 days. On the 76th day all rats were sacrificed and their testes were collected for histological slides using Hematoxylin-Eosin staining. The results showed that fasting treatment for five days (group K +) caused a decrease (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to those of the normal mice (group K–). Administration of 50% honey (group P2) for 10 days caused an increase (p <0.05) in the number of spermatogonia cells, spermatocytes and spermatids compared to the K + group mice. In P2 group, the number of spermatogonia cells was lower (p <0.05), while the number of spermatocytes and spermatids was not significantly different than in the K– group mice. It could be concluded that the administration of honey was able to regenerate the testicular tissue of rats with nutritional deficiency by increasing the number of spermatogenic cells in the seminiferous tubules.

show abstract

Section: Perlakuan Hewan Cobaunclassified

POTENSI MADU PADA PENINGKATAN JUMLAH SEL SPERMATOGENIK TIKUS (Rattus norvegicus) YANG KEKURANGAN NUTRISI

Listyorini

Hernawati²,

Suprayogi³

2021

Ovz

View full text Add to dashboard Cite

show abstract

“…At the indifferent stem stage, self-renewal is a symptom of the biological process and defense mechanism [23]. The homing signal based on the vascular endothelial growth factor (VEGF) expression following transplantation is critical for culture hypoxia-conditioned rat MSCs (1% O 2 concentration) [2]. VEGF is a component of the stem cell extracellular matrix that helps Available at www.veterinaryworld.org/Vol.14/November-2021/28.pdf maintain a favorable milieu for stem cells to survive after transplantation.…”

Section: Introductionmentioning

confidence: 99%

“…Mesenchymal stem cell (MSC) transplantation using rabbit [ 1 ] and rat [ 2 ] bone marrow and later rat [ 3 ] and rabbit [ 4 ] adipose tissue was shown to be effective in rebuilding the tissues supporting the endogenous stem cells, allowing them to multiply and mature into sperm cells. The viability of the transplanted MSCs from the bone marrow [ 2 , 5 ], adipose tissue [ 3 ], or umbilical cord blood [ 6 ] is low; hence, this treatment has limited efficacy. The reduced viability of MSCs is thought to be caused by a normoxia culture with a high oxygen concentration (O 2 >20%).…”

Section: Introductionmentioning

confidence: 99%

“…Other studies have indicated the critical significance of stem cell cultivation under hypoxic conditions as follows: To keep transplanted MSCs alive and adaptable, they were grown in a hypoxic environment (1-3% O 2 ) [ 2 , 4 ]. This condition was induced by cells in the quiescent state [ 4 , 16 , 17 ], allowing the cells to live longer [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

Safitri

Purnobasuki

2021

Vet World

Self Cite

View full text Add to dashboard Cite

Background and Aim: Mesenchymal stem cells (MSCs) transplanted into the testes of rats with testicular failure can help rescue fertility. However, the low viability of transplanted MSCs limits the success of this treatment. This study aimed to determine the effectiveness of MSCs cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus). Materials and Methods: Bone marrow-derived MSCs (200 million cells/rat) were transplanted into male rat models with induced infertility (10 rats/treatment group) after 4 days of culture in 21% O2 (normoxia) and 1% O2 (hypoxia). Ten fertile and 10 untreated infertile rats served as controls. In the infertile male rats that had been fasted from food for 5 days, the fasting condition induced malnutrition and then resulted in testicular failure. Results: The results indicated that the MSCs cultured under hypoxic conditions were more effective than those cultured in normoxic conditions as a treatment for testicular failure in infertile male rats based on the increased number of cells expressing p63 as a quiescent cell marker and ETV5 as a transcription factor expressed in Sertoli and germ cells. Furthermore, the structure of the seminiferous tubules, which contain spermatogonia, primary and secondary spermatocytes, and spermatid, Sertoli, and Leydig cells, was improved in infertile male rats treated with the MSCs cultured under hypoxic conditions. Conclusion: The testicular transplantation of MSCs cultured under hypoxic conditions was an effective treatment for testicular failure in rats.

show abstract

“…Multipotent of rabbit’s bone marrow-derived mesenchymal stem cells (BM-MSCs) can be induced to become pluripotent in vitro when cultured under low O 2 tension conditions [ 1 ]. Low O 2 tension is required to induce a favorable microenvironment to maintain MSCs in vitro , mimicking their BM niche [ 2 ], to ensure their viability for transplantation [ 3 ]. The low O 2 tension in the BM helps to maintain stem cells adjusted to the body’s normal physiological condition [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

Effect of low oxygen tension on transcriptional factor OCT4 and SOX2 expression in New Zealand rabbit bone marrow-derived mesenchymal stem cells

Safitri

2020

Vet World

Self Cite

View full text Add to dashboard Cite

Background and Aim: Octamer-binding transcription factor 4 (OCT4) and sex-determining region Y-box 2 (SOX2) are transcription factors whose functions are essential to maintain the pluripotency of embryonic stem cells. The purpose of this study was to derive stem cells for in vitro culture and to maintain their viability and pluripotency, with the goal to obtain a cell line for transplantation in patients with degenerative diseases or injuries. This research focused on examining the effect of low oxygen tension on the ability of bone marrow-derived mesenchymal stem cells (BM-MSCs) to express OCT4 and SOX2 in vitro. Materials and Methods: BM-MSCs were obtained from femurs of 2000 to 3000 g New Zealand male rabbits. BM-MSCs were divided into three groups to test different culture conditions: A control group under hyperoxia condition (21% O2) and two treatment groups with low oxygen tension (1% and 3% O2). We characterized the BM-MSCs using flow cytometric measurement of cluster differentiation 44 (CD44) and cluster differentiation 90 (CD90) expression. The expression of OCT4 and SOX2 was measured by immunofluorescence staining after 48 h of incubation in chambers with normal or low oxygen tension with controlled internal atmosphere consisting of 95% N2, 5% CO2, and 1% O2 (T1) and 3% O2 (T2). We considered OCT4 and SOX2 as two markers of pluripotency induction. All immunofluorescence data were subjected to a post hoc normality Tukey's honestly significant difference test; all differences with p<5% were considered significant. Results: BM-MSCs were positive for CD44 and CD90 expression after isolation. Oxygen tension culture conditions of 1% and 3% O2 led to OCT4 and SOX2 expression on culture days 2 and 4 (p<0.05), respectively, as compared to the hyperoxia condition (21% O2). Conclusion: Based on the OCT4 and SOX2 immunofluorescence data, we conclude that the stem cells were pluripotent at low O2 tension (at 1% O2 on day 2 and at 3% O2 on day 4), whereas under 21% O2 the OCT4 and SOX2 were not expressed.

show abstract

Comparison of biotechnological culture of hypoxia-conditioned rat mesenchymal stem cells with conventional in vitro culture of normoxia-conditioned rat mesenchymal stem cells for testicular failure therapy with low libido in rats

Cited by 11 publications

References 21 publications

POTENSI MADU PADA PENINGKATAN JUMLAH SEL SPERMATOGENIK TIKUS (Rattus norvegicus) YANG KEKURANGAN NUTRISI

POTENSI MADU PADA PENINGKATAN JUMLAH SEL SPERMATOGENIK TIKUS (Rattus norvegicus) YANG KEKURANGAN NUTRISI

Effectiveness of mesenchymal stem cells cultured under hypoxia to increase the fertility rate in rats (Rattus norvegicus)

Effect of low oxygen tension on transcriptional factor OCT4 and SOX2 expression in New Zealand rabbit bone marrow-derived mesenchymal stem cells

Contact Info

Product

Resources

About