Comparison of Blood Gas Analysis between Benchtop and Handheld Device

Abstract

The use of blood gas analysis is to determine the Acid-base status required to treat patients with emergency conditionssuch as metabolic disorders and respiratory diseases. Benchtop device is commonly used in hospitals to analyze blood gas;however, handheld devices are recently more often used in emergency settings due to its quick and simple process. Thisstudy was performed to compare blood gas analysis results between the i-STAT handheld device and the Nova pHox Ultrabenchtop device that were currently being used in the central laboratory. This cross-sectional study was conducted by using42 arterial blood patients that were measured with i-STAT handheld device dan Nova pHox Ultra benchtop device. The pH,pCO2, and pO2 parameters were then evaluated. The data were analyzed using Spearman's correlation test, Mann-Whitneytest, and Bland-Altman plots. This study showed a very strong positive correlation for all parameters. Mann-Whitneycomparison test showed that there was no significant difference between the result of the two devices (p-value > 0.05). Allparameters showed that 95% of plots were within the acceptable limit. There was no clinical significance on the mean biasesof blood gas results between both devices. The i-STAT and Nova pHox Ultra devices showed a good agreement for bloodgas measurement. Therefore, both devices can be used interchangeably with minimal effect on clinical decision-making.

Comparison of selected serum biochemistry measurements between the Nova Prime Plus VET, Nova pHOx Ultra, and Beckman Coulter AU680 analyzers in dogs

“…Method comparison studies have been conducted using human whole blood and serum samples for the Nova pHOx Ultra analyzer and the Nova Critical Care Xpress analyzer or i-STAT analyzer. [22][23][24][25] Good agreement was observed in the first two studies, whereas in the latter two studies, the Ultra direct ISE analyzer read whole blood Na concentration 4.9 and 4.3 mmol/L lower, and whole blood K concentration 0.25 and 0.04 mmol/L lower than indirect ISE autoanalyzers. 24,25 For comparison, the Ultra direct ISE analyzer read whole blood Na concentration 2.5 mmol/L lower, and whole blood K concentration 0.11 mmol/L higher than the indirect ISE Beckman chemistry analyzer in our study.…”

“…Finally, samples were not analyzed in duplicate, as recommended. 2 Blood gas chemistry sample analyses from critically ill animals are rarely run in duplicate 10,[22][23][24][25] because of the added cost and time in obtaining results and time-related changes in analytes. Consequently, single measurement was preferred in our study as it reflected the use of the analyzers in veterinary medicine.…”

“…These appear to be the first method comparison studies for the Nova Prime Plus VET and Nova pHOx Ultra blood gas chemistry analyzers in veterinary medicine. Method comparison studies have been conducted using human whole blood and serum samples for the Nova pHOx Ultra analyzer and the Nova Critical Care Xpress analyzer or i‐STAT analyzer 22‐25 . Good agreement was observed in the first two studies, whereas in the latter two studies, the Ultra direct ISE analyzer read whole blood Na concentration 4.9 and 4.3 mmol/L lower, and whole blood K concentration 0.25 and 0.04 mmol/L lower than indirect ISE autoanalyzers 24,25 .…”

“…Based on this information, we speculate that the variability in measured values by direct ISE on frozen serum samples can be decreased by collecting and storing the sample under conditions that minimize the potential for increased sample pH, thawing at 37°C, and ensuring adequate vortexing of the sample before measurement.Nevertheless, it is preferable to use fresh whole blood samples instead of aerobically collected and stored plasma or serum samples when measuring electrolyte concentrations using direct ISE methods.These appear to be the first method comparison studies for the Nova Prime Plus VET and Nova pHOx Ultra blood gas chemistry analyzers in veterinary medicine. Method comparison studies have been conducted using human whole blood and serum samples for the Nova pHOx Ultra analyzer and the Nova Critical Care Xpress analyzer or i-STAT analyzer [22][23][24][25]. Good agreement was observed in the first two studies, whereas in the latter two studies, the Ultra direct ISE analyzer read whole blood Na concentration 4.9 and 4.3 mmol/L lower, and whole blood K concentration 0.25 and 0.04 mmol/L lower than indirect ISE autoanalyzers 24,25.…”

Perbandingan Hasil Analisis Gas Darah Arteri Antara Alat Point of Care Testing (Poct) Dan Laboratory Blood Gas Analyzer Pasien Pneumonia

“…15 Hal ini sesuai dengan penelitian lainnya oleh Liana dkk (2019) menggunakan alat i-STAT dan Nova pHox Ultra dengan total 42 sampel terdapat korelasi yang bermakna (p<0,05) dan hubungan sangat kuat (r≥0,8). 16 Sebuah studi lainnya yang dilakukan oleh Boonlert dkk (2003) ditemukan adanya korelasi antara hasil pemeriksaan POCT analisis gas darah OPTI CCA dan OMNI 9 dengan alat analisis gas darah di rumah sakit (SP, RxL, CRT, san Cell Dyn) menunjukan adanya korelasi antara kedua hasil pemeriksaan. 17 Pada penelitian yang dilakukan oleh Lukkonen dkk (2015) menggunakan alat POCT EPOC dengan laboratory blood gas analyzer Rapidlab RL1265 dan Rapid point RP500, ditemukan adanya hubungan antara hasil analisis gas darah menggunakan alat POCT EPOC dengan laboratory blood gas analyzer Rapidlab RL1265 dan Rapid point RP500.…”