Comparison of Bone Formation in Rabbits Using Hydroxyapatite and β-Tricalcium Phosphate Scaffolds Fabricated From Egg Shells

Kim, S.H.; Kim, W.; Cho, J.H.; Oh, Nam Sik; Lee, Myung Hyun; Lee, Sang Jin

doi:10.4028/www.scientific.net/amr.47-50.999

Cited by 12 publications

(23 citation statements)

References 3 publications

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“…Eggshell-derived hydroxyapatite is hydrophilic in nature, absorbed by body fluids and blood, so that handling becomes easy for placing it in the surgical site. Because of ethical concerns collection of tissue for histologic examination has excluded in this study but histologic examination of EHA has been studied in animal models [16][17][18][19][20] and surface modified eggshell in humans which showed promising results. 21 The preliminary reports of EHA showed good results in humans by Kattimani et al 23 Other interesting trends for HA include applications in drug delivery, cell culture, purification of antibodies on industrial scale, as an artificial blood vessel or trachea, as well as a catheter made of an HAcomposite.…”

Section: Discussionmentioning

confidence: 96%

“…All the bone regeneration parameters were well correlated with the observations of the study performed by other investigators in the literature. [18][19][20]23 Eggshell-derived hydroxyapatite seems to be promising graft material with excellent properties for grafting [18][19][20]23 with viable various production techniques. [25][26][27][28][29][30][31][32][33][34][35] Lack of disease transfer risks, biocompatibility, and ease of use makes EHA a viable choice as regenerative material.…”

Section: Discussionmentioning

confidence: 99%

“…16,17 The eggshell powder has been examined in rats for bone healing. [16][17][18][19][20] Few studies show surface modified eggshell powder as osteoconductive bone filling material with variable benefit in bone regeneration. 16,17,21 The literature showed material properties of eggshell-derived hydroxyapatite (EHA) are superior when compared with the commercially available graft materials.…”

mentioning

confidence: 98%

“…23 Histomorphometric evaluation showed that the EHA has excellent new bone formation ability. [18][19][20][21] This has led to the curiosity to prepare the EHA from eggshell waste in a very economical way. [22][23][24] The aim of this study is to evaluate and compare the efficacy of indigenously prepared EHA with commercially available synthetic hydroxyapatite (SHA) for enhancement of the bone regeneration.…”

mentioning

confidence: 99%

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Eggshell-Derived Hydroxyapatite

Kattimani

Lingamaneni

Chakravarthi

et al. 2016

Journal of Craniofacial Surgery

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 98%

mentioning

confidence: 99%

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Eggshell-Derived Hydroxyapatite

Kattimani

Lingamaneni

Chakravarthi

et al. 2016

Journal of Craniofacial Surgery

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“…It has also been reported that calcium phosphate doped with two or more trace elements has shown better biologic performance such as accelerated mineralization and significantly higher bone formation [8]. The egg shell derived HA, β-TCP and biphasic calcium phosphates derived from waste has already been used as a bone graft substitute in in vivo models [9]. Due to the presence of various biologically relevant ions such as Mg 2+ , Sr 2+ , SiO [2][3][4] , F -, K + and Na + in an optimal composition, the egg shell derived bioceramics exhibits enhanced biological properties [6].…”

Section: Introductionmentioning

confidence: 99%

Self Setting Bone Cement Formulations Based on Egg shell Derived TetraCalcium Phosphate BioCeramics

phosphate¹,

shells²,

cements³

et al. 2015

Bioceram Dev Appl

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Egg shells have been used as a calcium source for synthesis of tetra calcium phosphate (ETTCP) by solid state reaction method. The cell parameters and cell volume of ETTCP measured by X-ray powder diffraction method were lower than the tetra calcium phosphate prepared using synthetic Ca(CO) 3 (pure TTCP) for comparison. The vibration bands of ETTCP were also slightly different from the vibration bands of pure TTCP characterized by Fourier transformed infrared spectroscopy. ETTCP has been tried as a main component in a self setting bone cement to evaluate the advantages of the presence of the biologically relevant ions such as Mg 2+ , Sr 2+ , SiO 2-4 , F -, K + and Na + ions in the cement properties. The setting time of the ETTCP derived cement was ~ 11 min compared to ~ 16 min of the pure TTCP derived cement. The amount of hydroxyapatite formed as the end product was about 12% higher for ETTCP derived cement than pure TTCP derived cement after 28 days of immersion in phosphate buffer solution as confirmed by phase analysis. Elemental analysis also indicates the presence of trace elements in minor concentration in ETTCP derived cement. Although both the cements showed similar compressive strength after 28 days, the initial strength of the ETTCP derived cement was remarkably higher during initial stages of the hardening reaction (24 h-7 days) compared to TTCP derived cement. Cell viability of L6 cells was higher and cell spreading was more for the ETTCP derived cement than pure TTCP derived cement. The present study has demonstrated the advantages of eggshell derived TTCP in bone cement formulations due to the presence of biologically relevant ions. This may help the clinician with brief surgical procedure by using faster setting cement as well as the patient to have quick recovery with a higher initial strength of cement.

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Injectable macroporous naturally‐derived apatite bone cement as a potential trabecular bone substitute

Dewangan,

Sampath Kumar,

Doble

et al. 2024

J Biomed Mater Res

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In this study, we have formulated a novel apatite bone cements derived from natural sources (i.e. eggshell and fishbone) with improved qualities that is, porosity, resorbability, biological activity, and so forth. The naturally‐derived apatite bone cement (i.e. FBDEAp) was prepared by mixing hydroxyapatite (synthesized from fishbone) and tricalcium phosphate (synthesized from eggshell) as a solid phase with a liquid phase (a dilute acidic blend of cement binding accelerator and biopolymers like gelatin and chitosan) with polysorbate (as liquid porogen) to get a desired bone cement paste. The prepared cement paste sets within the clinically acceptable setting time (≤20 min), easily injectable (>85%) through hands and exhibits physiological pH stability (7.3–7.4). The pure apatite phased bone cement was confirmed by x‐ray diffraction and Fourier transform infrared spectroscopy analyses. The FBDEAp bone cement possesses acceptable compressive strength (i.e. 5–7 MPa) within trabecular bone range and is resorbable up to 28% in simulated body fluid solution within 12 weeks of incubation at physiological conditions. The FBDEAp is macroporous in nature (average pore size ~50–400 μm) with interconnected pores verified by SEM and micro‐CT analyses. The FBDEAp showed significantly increased MG63 cell viability (>125% after 72 h), cell adhesion, proliferation, and key osteogenic genes expression levels (up to 5–13 folds) compared to the synthetically derived, synthetic and eggshell derived as well as synthetic and fishbone derived bone cements. Thus, we strongly believe that our prepared FBDEAp bone cement can be used as potential trabecular bone substitute in orthopedics.

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Comparison of Bone Formation in Rabbits Using Hydroxyapatite and β-Tricalcium Phosphate Scaffolds Fabricated From Egg Shells

Cited by 12 publications

References 3 publications

Eggshell-Derived Hydroxyapatite

Eggshell-Derived Hydroxyapatite

Self Setting Bone Cement Formulations Based on Egg shell Derived TetraCalcium Phosphate BioCeramics

Injectable macroporous naturally‐derived apatite bone cement as a potential trabecular bone substitute

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