Comparison of Botryosphaeran production by the ascomyceteous fungus Botryosphaeria sp., grown on different carbohydrate carbon sources, and their partial structural features

Abstract

The influence of glucose concentration and other carbohydrates (monosaccharides: fructose, galactose, mannose; polyols: mannitol and sorbitol; disaccharides: lactose, sucrose and commercial sucrose; and industrial sugarcane molasses) were compared as sole carbon sources for the production of Botryosphaeran, an exopolysaccharide (EPS) produced by Botryosphaeria sp. The optimum glucose concentration for EPS production was 50 g l(-1). With the exception of mannitol, the fungus produced EPS on all carbon sources studied, with highest yields occurring with sucrose followed by glucose. All EPS showed exclusively glucose after acid hydrolysis and monosaccharide analysis. FTIR spectroscopy demonstrated the presence of beta-anomers indicating that all the EPS produced by Botryosphaeria sp. on the different carbon sources were essentially of the beta-D-glucan type.

Produção e caracterização de biomassa extracelular obtida por fermentação submersa usando Lasidioplodia theobromae isolado do cacau

“…Após esse período, o meio foi ultracentrifugado sendo o sobrenadante precipitado em 3 volumes de etanol gelado. O peso da goma e da biomassa residual foi determinado por gravimetria e a fonte de carbono que apresentou melhor produção foi selecionada para os demais testes [15] .…”

“…Diferentes concentrações da fonte de carbono (10 g.L -1 -50 g.L -1 ) e o pH (4,0-8,0) do meio foram testadas, buscando avaliar a influência destes fatores na produção das macromoléculas fúngicas e o ensaio de maior produção foi selecionado para testes de caracterização [15] .…”

Novos compósitos de polianilina e exopolissacarídeos com aplicações em biomateriais: uma revisão

“…Os exopolissacarídeos (EPS) são definidos como polissacarídeos extracelulares, produzidos por alguns fungos e bactérias, os quais são encontrados ligados à superfície das células ou são excretados para o meio extracelular, na forma de limo (SUTHERLAND, 1998;STELUTI et al 2004).…”

Soybean Oil and Meal as Substrates for Lipase Production by Botryosphaeria ribis, and Soybean Oil to Enhance the Production of Botryosphaeran by Botryosphaeria rhodina

“…The side branches consist of glucosyl and gentiobiosyl residues linked to the β-1,3-D-glucan backbone chain by β-1,6-bonds (Barbosa et al, 2003). When grown on different carbohydrate substrates, Botryosphaeria rhodina MAMB-05 produced a family of botryosphaerans that differed only in the degree of branching (Steluti et al, 2004). Botryosphaeria rhodina MAMB-05 grown on fructose presented a higher degree of branching (31 %) compared to that when grown on sucrose and glucose (21-22 %) as carbon sources.…”

“…Botryosphaeran production by Botryosphaeria rhodina MAMB-05 and its isolation from the ECF through precipitation with ethanol was developed as described by Steluti et al, (2004), and Silva et al, (2007).…”