Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study

Taylor, T.H.; Elliott, Thomas; Colturato, L.F.; Straub, R.J.; Mitchell-Leef, Dorothy; Nagy, Zsolt

doi:10.1016/j.fertnstert.2005.10.053

Cited by 23 publications

(9 citation statements)

References 7 publications

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“…Oocytes were retrieved, trimmed of blood, and stripped of cumulus cells as described by Taylor et al (2006). Oocytes were graded for maturity, separated, placed into a 60-mm dish (Thermo scientific, Rochester, New York, USA) containing 250 µL drops of continuous single culture (CSC; Irvine Scientific, Santa Ana, California, USA) supplemented with 10% serum substitute supplement (SSS; Irvine Scientific, Santa Ana, California, USA) and overlaid with 8 ml oil (Irvine Scientific, Santa Ana, California, USA).…”

Section: Embryo Culturementioning

confidence: 99%

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Taylor

Patrick

Gitlin

et al. 2014

Reproductive BioMedicine Online

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Comprehensive chromosome screening is typically used for aneuploidy analysis of blastocysts. It is believed that either day of blastocyst development is acceptable. Euploidy rates and outcomes were examined between day 5 and day 6 blastocysts in two studies. First, euploidy rates of day 5 and day 6 blastocysts were examined on a per-embryo and per-patient basis. Second, outcomes were compared when only euploid day 5 or day 6 blastocysts were transferred in a cryopreserved embryo transfer cycle. In cycles (n = 70) that had blastocysts biopsied on both day 5 and day 6, day 5 blastocysts had a higher chance of being euploid than day 6 blastocysts (125/229 [54.6%]) and (77/180 [42.8%]), respectively (P = 0.0231). Similarly, euploid rates in blastocysts from patients (n = 193) with day 5 biopsy, day 6 biopsy, or both, were significantly higher in day 5 (235/421 [55.8%]) compared with day 6 (184/413 [44.6%]) blastocysts (P = 0.0014). In the second study, 50 women (36.1 ± 4.3 years) and 39 women (35.1 ± 3.8 years) with only euploid day 5 or euploid day 6 blastocysts transferred during a cryopreserved embryo transfer had similar cycle outcomes. Although underpowered, these data suggest that euploid day 6 blastocysts are as capable of positive outcomes as their euploid day 5 counterparts.

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Section: Embryo Culturementioning

confidence: 99%

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Taylor

Patrick

Gitlin

et al. 2014

Reproductive BioMedicine Online

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“…Ever since only few studies were performed aiming to correlate the possible toxic effects of hyaluronidase in several laboratorial parameters of in vitro fertilization. Other studies reported some trials using lower concentrations, such as 40 and 20 IU/mL, but they only compared this bovine-derived enzyme to the recombinant enzyme, not supporting our findings, but they also suggest some possible effects of toxicity that could influence fertilization rates and embryo quality (De Vos et al ., 2008; Taylor et al ., 2006). …”

Section: Discussionmentioning

confidence: 99%

Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality

Moura¹,

Gurgel

Machado³

et al. 2017

JBRA Assisted Reproduction

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ObjectiveHyaluronidase enzyme is an extremely important factor for the process of oocyte denudation, but little is known about its negative effects.MethodsThis prospective randomized study analyzed the results of using different concentrations of hyaluronidase (Diluted: 8IU/mL and Normal: 80IU/mL) used for denudation of sibling-oocytes for 22 women undergoing treatment for assisted reproduction by ICSI. A total of 192 oocytes were injected, being 104 for group I (diluted) and 88 for group II (normal). We analyzed fertilization rate, cleavage, embryo quality at 48 and 72 hours and number of transferred embryos in each group.ResultsThe diluted enzyme group showed better results in fertilization rates (92.3% vs. 80.6%), mean cleavage (4.18 ± 2.57 vs. 3.09 ± 1.90), in 48-hour embryos A and A + B (60.9% vs. 44.1% and 90.2% vs. 82.3%) and at 72 hours (45.6% vs. 36.8% and 77.1% vs 66.2%), and number of embryos selected for transfer (61.8% vs. 38.1%). The overall pregnancy rate was 59.1%.ConclusionThis study demonstrates that the use of 8 IU/mL of hyaluronidase, according to the following protocol, is beneficial and can be successfully used for oocyte denudation, and it is also economically advantageous to the laboratory

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“…80 Because of its purity, recombinant human hyaluronidase, ICSI Cumulase® (Origio), may be used at higher concentrations; as a result, the incubation time becomes less critical. 81 For these reasons, this product may reduce, if not eliminate, the risk of damage or trauma to the oocytes by the mechanical denudation step. Additionally, the risk of animal pathogen contamination is alleviated.…”

Section: Handling Of Oocytesmentioning

confidence: 99%

Gamete and Embryo Manipulation

Bormann¹

2014

Yen &Amp; Jaffe's Reproductive Endocrinology

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Comparison of bovine- and recombinant human-derived hyaluronidase with regard to fertilization rates and embryo morphology in a sibling oocyte model: a prospective, blinded, randomized study

Cited by 23 publications

References 7 publications

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Comparison of aneuploidy, pregnancy and live birth rates between day 5 and day 6 blastocysts

Low concentration of hyaluronidase for oocyte denudation can improve fertilization rates and embryo quality

Gamete and Embryo Manipulation

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