Comparison of Bovine, Simian and Human Rotavirus Structural Glycoproteins

Kouvelos, Kallie; Petric, Martin; Middleton, Peter J.

doi:10.1099/0022-1317-65-7-1211

Cited by 10 publications

(9 citation statements)

References 18 publications

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“…Depending on the strain of rotavirus, gene segment 8 or 9 codes for VP7 (25,180). For example, gene segment 8 codes for VP7 in the human serotype 2 rotavirus Hu/5 isolated in Melbourne, Australia (95), and for the bovine rotavirus UK (207). Gene segment 9 codes for VP7 in the human serotype 1 rotavirus Wa and for the simian rotavirus SA11 (180,183,207).…”

Section: Molecular Biology and Classificationmentioning

confidence: 99%

Human viral gastroenteritis.

Christensen¹

1989

Clinical Microbiology Reviews

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CHRISTENSEN propagated by using special cell types or techniques or both (320, 353, 372). In addition, a variety of in vitro detection systems, including enzyme immunoassays (149, 175, 333, 408), radioimmunoassays (137, 141, 181, 264), latex agglutination (140, 169), and nucleic acid hybridization techniques (116, 175), have been developed for their rapid detection. The detection and identification of these agents are important since viral gastroenteritis is the second most common clinical entity in developed countries, second only to viral upper respiratory tract illness (188). Worldwide, acute gastroenteritis and its associated dehydration afflicts almost 500 million children annually. In underdeveloped or developing countries, acute gastroenteritis, including viral gastroenteritis, is the leading cause of death of children under the age of 4 years (360). Since 1980, a wealth of literature has developed covering many aspects of these viruses and the infections they cause, including their molecular biology, epidemiology, immunology, and clinical features. In addition, detection tests for routine use have been developed for rotaviruses (310) and fecal adenoviruses (333) and experimental rotavirus vaccines have been developed (184, 377, 379, 380). Of these viruses, rotaviruses are the most common known cause of viral gastroenteritis in infants and young children, with the fastidious fecal adenoviruses most likely being the second most common cause in this age group (301, 369). Calici-, astro-, and coronaviruses are probably responsible for a minority of illness in the young age group (253, 302). In contrast, the Norwalk and Norwalk-like viruses have caused considerable numbers of outbreaks of gastroenteritis among older children, adolescents, and adults (136, 137, 189). ROTAVIRUSES Epidemiology Rotaviruses were first discovered in humans 15 years ago by Bishop et al. (18, 19) by the EM examination of duodenal

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Section: Molecular Biology and Classificationmentioning

confidence: 99%

Human viral gastroenteritis.

Christensen¹

1989

Clinical Microbiology Reviews

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“…Second, some parts of VP7 could be covered by carbohydrate that might mask antigenic epitopes in a manner analogous to that described for the influenza hemagglutinin (37). However, glycosylation is unlikely to result in the masking of the whole surface of SAl1 VP7, because the molecule possesses but a single glycosylation site: clearly, there is room on the surface for more than one carbohydrate moiety, since other strains have multiple glycosylation sites which are filled (22,23). Third, the regions of VP7 on the VOL.…”

Section: -mentioning

confidence: 99%

Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis

Gunn¹,

Sato²,

Powell³

et al. 1985

J Virol

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The rotavirus neutralizing antigen, VP7, is a 37,000-molecular-weight glycoprotein which is a major component of the outer shell of the virion. The amino acid sequence of VP7 for strain S2 (human serotype 2) and Nebraska calf diarrhea virus (bovine serotype) has been inferred from the nucleic acid sequence of cloned copies of genomic segment nine. Comparison of the amino acid sequences of these two VP7 proteins with those already determined for other rotavirus strains reveals extensive sequence conservation between serotypes with clusters of amino acid differences sited predominantly in hydrophilic domains of the protein. Six peptides have been synthesized that span the hydrophilic regions of the molecule. Antisera to these peptides both recognize the respective homologous peptides in a solid-phase radioimmunoassay and bind to denatured VP7 in a Western blot. However, none of the antisera either recognize virus or exhibit significant neutralizing activity, indicating that these peptide sequences are not available on the surface of the virus.

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“…However, some monoclonal antibodies react with viruses in both subgroups, so VP6 proteins must also share common epitopes. VP7 is the major outer capsid protein which is glycosylated and has an approximate molecular weight of 34,000 to 40,000 (93,106,163,180,183,207,275,335,350). This protein is responsible for the serotype specificity of the virus (10,92,94,95,106,163,180,337), which was initially determined by virus neutralization (166,402,404).…”

Section: Molecular Biology and Classificationmentioning

confidence: 99%

“…For example, gene segment 8 codes for VP7 in the human serotype 2 rotavirus Hu/5 isolated in Melbourne, Australia (95), and for the bovine rotavirus UK (207). Gene segment 9 codes for VP7 in the human serotype 1 rotavirus Wa and for the simian rotavirus SA11 (180,183,207). VP4 is an outer capsid protein which has an apparent molecular weight of 88,000 (241).…”

Section: Molecular Biology and Classificationmentioning

confidence: 99%

Human viral gastroenteritis

Christensen

1989

Clin Microbiol Rev

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During the last 15 years, several different groups of fastidious viruses that are responsible for a large proportion of acute viral gastroenteritis cases have been discovered by the electron microscopic examination of stool specimens. This disease is one of the most prevalent and serious clinical syndromes seen around the world, especially in children. Rotaviruses, in the family Reoviridae, and fastidious fecal adenoviruses account for much of the viral gastroenteritis in infants and young children, whereas the small caliciviruses and unclassified astroviruses, and possibly enteric coronaviruses, are responsible for significantly fewer cases overall. In addition to electron microscopy, enzyme immunoassays and other rapid antigen detection systems have been developed to detect rotaviruses and fastidious fecal adenoviruses in the stool specimens of both nonhospitalized patients and those hospitalized for dehydration and electrolyte imbalance. Experimental rotavirus vaccines have also been developed, due to the prevalence and seriousness of rotavirus infection. The small, unclassified Norwalk virus and morphologically similar viruses are responsible for large and small outbreaks of acute gastroenteritis in older children, adolescents, and adults. Hospitalization of older patients infected with these viruses is usually not required, and their laboratory diagnoses have been limited primarily to research laboratories.

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Comparison of Bovine, Simian and Human Rotavirus Structural Glycoproteins

Cited by 10 publications

References 18 publications

Human viral gastroenteritis.

Human viral gastroenteritis.

Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis

Human viral gastroenteritis

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