Comparison of Brazilian Plants Used to Treat Gastritis on the Oxidative Burst ofHelicobacter pylori-Stimulated Neutrophil

Abstract

Ten Brazilian medicinal plants used to treat gastritis and ulcers were carefully selected on the basis of ethnopharmacological importance and antiulcerogenic activity previously described. The antioxidant activity of the methanolic extracts was determined in analysis conditions that simulate a real biological activity on inhibition of the oxidative burst induced in neutrophils using Helicobacter pylori as activator, by a luminol-amplified chemiluminescence assay. The extracts, at low concentration (5 μg/mL), exhibited a large variation in inhibitory effects of H. pylori-induced oxidative burst ranging from 48% inhibition to inactive, but all extracts, excluding Byrsonima intermedia, had inhibitory activity over 80% at the concentration of 100 μg/mL. The total suppressive antioxidant capacity measured as the effective concentration, which represents the extract concentration producing 50% inhibition of the chemiluminescence induced by H. pylori, varies from 27.2 to 56.8 μg/mL and was in the following order: Qualea parviflora > Qualea multiflora > Alchornea triplinervia > Qualea grandiflora > Anacardium humile > Davilla elliptica > Mouriri pusa > Byrsonima basiloba > Alchornea glandulosa > Byrsonima intermedia. The main groups of compounds in tested extracts are presented. Differences in the phytochemical profile, quantitatively and qualitatively, of these plants can explain and justify their protective effect on the gastric mucosa caused by the neutrophil-generated ROS that occurs when H. pylori displays its evasion mechanisms.

Medicinal plants in the treatment ofHelicobacter pyloriinfections

“…Arg (Euphorbiaceae) are commonly used in folk medicine for the cure of gastric disorders . Recently, the antibacterial , anti-inflammatory (Lopes et al, 2010), antioxidant (Bonacorsi et al, 2013), antisecretory, gastroprotective, and anti-H. pylori effects of this species were reported. Alchornea triplinervia methanol extract was found to display antibacterial activity against the standard strain of H. pylori.…”

Natural Products for the Prevention and Management of Helicobacter pylori Infection

“…It was demonstrated that 5-methoxy-3,4dehydroxanthomegnin from Paepalanthus latipes (Kitagawa, Bonacorsi, Da Fonseca, Vilegas, & Raddi, 2012), and the methanol extracts of Qualea parviflora and Qualea multiflora (Bonacorsi, Da Fonseca, Raddi, Kitagawa, & Vilegas, 2013) could inhibit H. pylori-induced oxidative burst in rats, and the latter two showed activities at low concentrations (5 μg/mL). In another study, methyl gallate from Byrsonima crassa showed the highest inhibitory effects on oxidative burst of H. pylori-stimulated neutrophils (IC 50 : 3.4 μg/mL), among all the phenolic compounds and methanol extract from B. crassa (Bonacorsi, Raddi, Da Fonseca, Sannomiya, & Vilegas, 2012).…”

Dissimilarity between Mouriri elliptica (Mart.) plants cultivated in vitro and in situ through anatomic parameters

“…M. elliptica leaves and/or bark have been used to treat gastric ulcers and gastritis, and they may also have an antimicrobial effect. These medicinal effects stem from stimulation of the immune system in response to pathogens (Moleiro et al, 2009;Vasconcelos et al, 2010b) and from the inhibition of the oxidative capacity of Helicobacter pylori (Bonacorsi et al, 2013).…”