Comparison of Brilliant Green Agar and Hektoen Enteric Agar Media in the Isolation of Salmonellae from Food Products

Abstract

Brilliant Green (BG) agar and Hektoen enteric (HE) agar media were compared for their efficiency in isolating salmonellae from various food products. Of the 11,226 food specimens examined, 1,662 (or 14.9%) yielded salmonellae. Of this number, 1,475 (88.7%) were recovered from BG agar and 1,315 (79.1%) were recovered from HE agar media. The results indicate that BG agar is more effective in isolating salmonellae from food products. A smaller subsidiary study showed HE agar to be more selective than BG agar. Four hundred ten specimens yielded 92 nonlactose-fermenting isolants other than salmonellae on BG agar and only 11 such isolants on HE agar.

Comparison of selective enrichment and plating media for Salmonella isolation from broiler carcasses

“…(USDA, 2019). Hektoen Enteric (HE) agar inhibits gram‐positive bacteria and differentiates Salmonella using bile salts, bromothymol blue, and acid fuchsin dyes to produce blue to blue‐green colonies with a black center (Chang et al, 1999; Goo, Ching, & Gooch, 1973; King et al, 2005; Kristensen, Lester, & Jürgens, 1925).…”