Comparison of Brucella immunoglobulin M and G flow assays with serum agglutination and 2-mercaptoethanol tests in the diagnosis of brucellosis

Zeytinoğlu, Ayşın; Turhan, Ajda; Altuğlu, İmre; Bilgiç, Altınay; Abdoel, Theresia H.; Smits, Henk L.

doi:10.1515/cclm.2006.033

Cited by 15 publications

(10 citation statements)

References 7 publications

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“…Hence, in the present investigation, LFA was evaluated with RBPT and iELISA considering cELISA as the gold standard. Our results are in agreement with the high sensitivity and specificity observed for the flow assay by earlier reports from Spain [ 27 ], Turkey [ 28 , 29 ] Kazakhstan [ 28 ], and Egypt [ 30 , 31 ]. The LFA has shown good PPV and NPV greater than RBPT and almost similar to that of iELISA.…”

Section: Discussionsupporting

confidence: 93%

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Shome¹,

Filia²,

Padmashree³

et al. 2015

Vet World

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Aim:The aim was to evaluate lateral flow assay (LFA) as a field test for investigation of brucellosis outbreak in organized buffalo farm.Materials and Methods:A total of 153 serum samples were tested to detect the presence of brucella antibodies by LFA and three other serological tests i.e. rose bengal plate test (RBPT), protein G based indirect enzyme-linked immunoassay (iELISA), and competitive ELISA (cELISA). The performances of LFA and other serological tests were evaluated using OIE complaint cELISA as the gold standard.Results:Serological tests revealed 50% of the animals were seropositive for Brucella antibodies and correlated with clinical history of abortions, infertility, and productive failures. The newly developed assay showed 87.1% and 92.6% sensitivity and specificity, which was even higher than the specificity of RBPT.Conclusions:The investigation proved the potential usefulness of LFA for field diagnosis of brucellosis in the regions where laboratory facilities are limited.

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Section: Discussionsupporting

confidence: 93%

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Shome¹,

Filia²,

Padmashree³

et al. 2015

Vet World

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“…This could be an important aspect in countries such as Egypt where the disease is widely spread. Furthermore, our results are in agreement with the high sensitivity and specificity observed for the flow assay by earlier reports from Spain [19], Turkey [21,22], and Kazakhstan [21]. Therefore, in the absence of the laboratory support, the flow assay may be used for the confirmation of brucellosis in febrile patients with clinical suspicion of brucellosis by testing a drop of blood collected by finger prick.…”

Section: Discussionsupporting

confidence: 91%

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Marei

Boghdadi

Abdel-Hamed

et al. 2011

J Infect Dev Ctries

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Introduction: Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-ofcare test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. Methodology: Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year period were collected. All samples were subjected to the Brucella IgM/IgG lateral flow assay, serum agglutination test (SAT), rose bengal RB Test (RB), 2-mercapteoethanol (2-ME), culture and PCR. SAT, 2-ME, culture and PCR were retested after the end of the treatment. Results: Culture and SAT confirmed the diagnosis of brucellosis in twenty patients. While 90% of the samples were positive by SAT, only 30% and 85% were positive by culture and PCR respectively. The sensitivity of the lateral flow assay calculated for the Brucella IgM/IgG was 95% and specificity was 97%. Conclusion: These data show that the lateral flow assay is more suitable for diagnosis of brucellosis in Egypt than culture and SAT. Application of the PCR on serum samples collected during follow-up revealed that the DNA of the pathogen was yet not completely cleared almost 60 days after the start of treatment with doxycycline and ciprofloxacin.

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“…The Brucella lateral flow assay proved to be slightly more sensitive in detecting low levels of specific IgG or IgM antibodies than SAT [66].…”

Section: The Sat Rbt and Lateral Flow Assaymentioning

confidence: 93%

Implications of laboratory diagnosis on brucellosis therapy

Dahouk

Nöckler

2011

Expert Review of Anti-infective Therapy

167

131

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Brucellosis is one of the world's most widespread bacterial zoonoses, leading to tremendous economic losses in endemic regions and serious complaints in affected patients. The infection may be transmitted by direct animal contact, but is usually acquired through the consumption of contaminated food products of animal origin, mainly via unpasteurized goat's milk and cheese. Furthermore, brucellosis is the most common bacterial laboratory-acquired infection worldwide [1].Although many national and international programs have been established to eradicate the pathogen and control its spreading in animal husbandry, brucellosis is still a re-emerging disease. The surveillance of animal brucellosis is difficult due to bacterial persistence in wildlife and environmental reservoirs, with consecutive spill-over to domestic animals [2].Brucellosis is caused by members of the genus Brucella (B.), which are gram-negative, facultative intracellular coccobacilli that were historically differentiated by their preferred animal host, varying pathogenicity and a few selected phenotypic traits. The genus comprises six classical species: B. melitensis bv 1-3 (primarily isolated from sheep and goats); B. abortus bv 1-6 and 9 (from cattle and other Bovidae); B. suis bv 1-3 (from pigs), bv 4 (from reindeer), bv 5 (from small rodents); B. canis (from dogs); B. ovis (from sheep); and B. neotomae (from desert wood rats). Recently, two novel species of marine origin, B. pinnipedialis (isolated from seals) and B. ceti (from dolphins and whales) [3], B. microti isolated from the common vole (Microtus arvalis) [4], red foxes (Vulpes vulpes) [5] and from soil [6], and B. inopinata isolated from a breast implant wound of a 71-year-old female patient [7] have been described. In the past, a lot of atypical Brucella strains arose. These could represent novel species or lineages of already described species, for example various Brucella strains originating from wild native rodent species in North Queensland, Australia [8], a novel Brucella isolate in association with two cases of stillbirth in nonhuman primates [9], and a B. inopinata-like strain (BO2), which was isolated from a lung biopsy of a 52-year-old Australian patient suffering from chronic destructive pneumonia [10].Physicians' awareness of the infection is very poor in many countries, and most cases correctly identified are clinically advanced. Because of its protean clinical manifestations, human brucellosis can be easily confused with other infectious and noninfectious diseases, leading to diagnostic delays and late onset of therapy. The isolation of the fastidious organisms is often unsuccessful or takes a long time, which is why the presumptive clinical diagnosis is usually confirmed by Brucellosis is a worldwide zoonosis with a huge economic impact on animal husbandry and public health. The diagnosis of human brucellosis can be protracted because the disease primarily presents as fever of unknown origin with unspecific clinical signs and symptoms. The isolation rate of the fastidiou...

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Comparison of Brucella immunoglobulin M and G flow assays with serum agglutination and 2-mercaptoethanol tests in the diagnosis of brucellosis

Cited by 15 publications

References 7 publications

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Evaluation of lateral flow assay as a field test for investigation of brucellosis outbreak in an organized buffalo farm: A pilot study

Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

Implications of laboratory diagnosis on brucellosis therapy

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