Comparison of Buffalo green monkey kidney cells and McCoy cells for the isolation of Chlamydia trachomatis in shell vial centrifugation culture

Johnston, Sheryl L.G.; Siegel, Charles S.

doi:10.1016/0732-8893(92)90023-m

Cited by 9 publications

(5 citation statements)

References 13 publications

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“…Interestingly, our strain was obtained in endothelial cell cultures, whereas McCoy cells are usually recommended for isolation of this species from clinical specimens (3). C. trachomatis has been grown in vitro in McCoy cells (28,29,31), HeLa cells (13,23), and BGMK cells (10,12), including in shell vial systems (10,29), but to our knowledge this is the first time growth in endothelial cells has been reported for diagnostic purposes. This is compatible with a recent demonstration that Chlamydia pneumoniae and also C. trachomatis can infect human umbilical vein endothelial cells in vitro (7).…”

Section: Discussionmentioning

confidence: 98%

Isolation in Endothelial Cell Cultures of Chlamydia trachomatis LGV (Serovar L2) from a Lymph Node of a Patient with Suspected Cat Scratch Disease

Maurin

Raoult

2000

J Clin Microbiol

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An inguinal lymph node, removed from a 21-year-old Romanian man suspected of having cat scratch disease, was sent to our laboratory for Bartonella culture. Lymph node specimens were inoculated on blood-enriched agar and in an endothelial cell culture system using the centrifugation shell vial technique. Bacteria were grown in cell monolayers and detected as positive with an anti-Bartonella henselae rabbit serum. However, such bacteria were identified as Chlamydia trachomatis biovar LGV serovar L2 by PCR sequencing techniques. Pathological examination of tissue biopsies was compatible with either lymphogranuloma venereum or cat scratch disease. The shell vial system is suitable for isolation of intracellular pathogens responsible for chronic lymphadenopathies, including C. trachomatis, Bartonella species, Francisella tularensis, and mycobacteria. However, care should be taken when identifying Chlamydia spp. and Bartonella spp. using polyclonal antibodies, since species of both genera have common antigens which are responsible for cross-reactions.

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Section: Discussionmentioning

confidence: 98%

Isolation in Endothelial Cell Cultures of Chlamydia trachomatis LGV (Serovar L2) from a Lymph Node of a Patient with Suspected Cat Scratch Disease

Maurin

Raoult

2000

J Clin Microbiol

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“…Cell monolayers for culture of C. trachomatis are grown in dram or shell vials on glass coverslips or in the wells of multiwell cell culture dishes (132). Traditionally, McCoy (195,200,242), HeLa 229 (108,170), and, more recently, BGMK (89,106) cells have been used to support the growth of C. trachomatis. The shell vial method of culture is more sensitive for clinical specimens than the use of 96-well microculture plates when McCoy cells and growth medium with cycloheximide are used (200,242).…”

Section: Laboratory Methodsmentioning

confidence: 99%

Current methods of laboratory diagnosis of Chlamydia trachomatis infections

Black

1997

Clin Microbiol Rev

426

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Infections caused by Chlamydia trachomatis are probably the most common sexually transmitted diseases in the United States. Commonly unrecognized and often inadequately treated, chlamydial infections can ascend the reproductive tract and cause pelvic inflammatory disease, which often results in the devastating consequences of infertility, ectopic pregnancy, or chronic pelvic pain. C. trachomatis infections are also known to increase the risk for human immunodeficiency virus infection. The obligate intracellular life cycle of C. trachomatis has traditionally required laboratory diagnostic tests that are technically demanding, labor-intensive, expensive, and difficult to access. In spite of these historical challenges, however, laboratory diagnosis of C. trachomatis has been a rapidly advancing area in which there is presently a wide array of commercial diagnostic technologies, costs, manufacturers. This review describes and compares the diagnostic methods for C. trachomatis infection that are currently approved for use in the United States, including the newest DNA amplification technologies which are yet to be licensed for commercial use. Issues to consider in selecting a test for purposes of screening versus diagnosis based on prevalence, performance, legal, social, and cost issues are also discussed.

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“…Cervical swabs were collected and placed into a viral transport media (M4RT; Remel Products) and then maintained at 270°C. Chlamydia culture was performed in dram vials containing Buffalo Green Monkey Kidney Cells (BGMK) (Diagnostic Hybrids) following a modification of Krech [14,15]. Briefly, after aspiration of maintenance medium, duplicate BGMK vials were inoculated with 200 lL of vortexed specimen.…”

Section: Trachomatis Detection: Culturementioning

confidence: 99%

The Effects of a Single Cervical Inoculation of Chlamydia trachomatis on the Female Reproductive Tract of the Baboon (Papio anubis)

Bell¹,

Bergin²,

Harris³

et al. 2011

The Journal of Infectious Diseases

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Comparison of Buffalo green monkey kidney cells and McCoy cells for the isolation of Chlamydia trachomatis in shell vial centrifugation culture

Cited by 9 publications

References 13 publications

Isolation in Endothelial Cell Cultures of Chlamydia trachomatis LGV (Serovar L2) from a Lymph Node of a Patient with Suspected Cat Scratch Disease

Isolation in Endothelial Cell Cultures of Chlamydia trachomatis LGV (Serovar L2) from a Lymph Node of a Patient with Suspected Cat Scratch Disease

Current methods of laboratory diagnosis of Chlamydia trachomatis infections

The Effects of a Single Cervical Inoculation of Chlamydia trachomatis on the Female Reproductive Tract of the Baboon (Papio anubis)

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