Comparison of Calbindin D‐28k and S‐100 Protein B in Neuroblastoma as Determined by Enzyme Immunoassay

Abstract

Levels of two calcium‐binding proteins, calbindin D‐28k (calbindin‐D) and S‐100 protein B (S‐100b), were measured by immunoassay in solid tumors obtained surgically from pediatric patients. Mean concentrations of calbindin‐D and S‐100b in 73 neuroblastomas (23 ganglioneuroblastomas and 50 neuroblastomas) were 10‐ or 25‐fold higher, respectively, than those in other types of solid tumors in pediatric patients (n=15). The mean tumor concentration of calbindin‐D in patients with neuroblastoma (n=73) was 25.1 ng/mg (range 0.20 to 317.0 ng/mg soluble protein, SE=6.26); that of S‐100b was 278.3 ng/mg (range 0.93 to 2521 ng/mg soluble protein, SE=71.7). The mean concentration of calbindin‐D (4.4 ng/mg soluble protein) was significantly (P < 0.05) lower in stage IV, the most advanced stage. The mean concentration of S‐100b (74.0 ng/mg soluble protein) was lower in patients with undifferentiated neuroblastomas (P < 0.01). Tumor levels of the two calcium‐binding proteins were not correlated in patients with neuroblastoma, but each was strongly correlated with outcome in patients with neuroblastoma. The evidence suggests that measurements of the calcium‐binding proteins calbindin‐D and S‐100b would be useful for evaluating the prognosis of patients with neuroblastoma.

S-100 and MATH-1 Protein Expressions Can Be Useful for the Prediction of Clinical Outcome in Neuroblastoma Patients

“…The function of S-100 proteins has been investigated in several cancer types including NB. [14][15][16][17] A study conducted by Leclerc et al 14 reported that S-100A6 promotes the survival of NB cells by interacting with RAGE (receptor for advanced glycation end products) receptor. Another study with NB histologic sections showed that S-100 positivity of the cells in tumor tissue can be related to the favorable clinical outcome.…”

“…16 S-100B expression in NB was shown to be able to predict the clinical outcome of the patients. 17 MATH-1 (also called ATOH1; Atonal Homolog 1) protein is a basic helix-loop-helix (bHLH) transcription factor mainly expressed in neuronal progenitor cells of the nervous system. It plays role in neuronal cell differentiation through development.…”

Processing characteristics and physicomechanical properties of natural rubber and liquid natural rubber blends

“…Calbindin‐D 28k expression was also found in a subset of medulloblastomas presenting poorly differentiated cells but was absent in medulloblastomas with desmoplastic characteristics 46. Measurement of calbindin‐D 28k and S100 proteins has also been proposed to be useful for evaluating the prognosis of children suffering from neuroblastoma, a neuro‐ectodermal derived peripheral tumor,47 and recently calbindin‐D 28k was found to be a prognostic factor in lung carcinoma 39…”

Calbindin‐D28k

“…Calbindin-D 28k expression was also found in a subset of medulloblastomas presenting poorly differentiated cells but was absent in medulloblastomas with desmoplastic characteristics. 46 Measurement of calbindin-D 28k and S100 proteins has also been proposed to be useful for evaluating the prognosis of children suffering from neuroblastoma, a neuroectodermal derived peripheral tumor, 47 and recently calbindin-D 28k was found to be a prognostic factor in lung carcinoma. 39 In the current study, four Ca 2ϩ -binding proteins (calbindin-D 28k , calretinin, ␣-parvalbumin, and ␤-parvalbumin) exhibited different patterns of expression in the blue round cells of the 39 medulloblastomas analyzed (see Table 2).…”