Comparison of capillary zone electrophoresis with ion chromatography and standard photometric methods for the determination of inorganic anions in atmospheric aerosols

Da̧bek-Złotorzyńska, Ewa; Dlouhy, Joseph F.; Houle, Nicole; Piechowski, Maria; Ritchie, Scott

doi:10.1016/0021-9673(94)01108-q

Cited by 30 publications

(13 citation statements)

References 13 publications

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“…Filters used in air sampling provide such a challenge, with anions such as phosphate, methanesulfonate and oxalate being present in low concentrations and chloride, sulfate and acetate occurring at concentrations an order of magnitude greater. The analysis of air filter samples for anions is traditionally performed using ion chromatography [18][19][20], with relatively few uses of CE for such samples having been reported [21][22][23][24][25]. DabekZlotorzynska et al [21,24,25] used a pyromellitate electrolyte and indirect detection at 254 nm to determine nitrate, nitrite, chloride, oxalate, and sulfate in air filter samples.…”

Section: Analysis Of Air Filter Samplesmentioning

confidence: 98%

Sensitive indirect photometric detection of inorganic and small organic anions by capillary electrophoresis using Orange G as a probe ion

et al. 2003

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This study addresses the two major problems in the use of dyes as highly absorbing probes for indirect photometric detection in capillary electrophoresis (CE). First, effective electroosmotic flow (EOF) modification or suppression to allow separation and detection of a wide mobility range of analytes is not straightforward when electrolytes containing increased dye concentrations are used. The suppression of EOF to less than + 5x10(-9) m(2)V(-1)s(-1) was achieved with a combination of a poly(ethylenimine) (PEI)-coated capillary and the addition of the neutral polymer hydroxypropylmethylcellulose (HPMC) to the background electrolyte. Second, the deterioration of baselines due to adsorption of the dye probe to the capillary wall is generally a problem. In this work, baseline quality at higher probe concentrations was significantly improved by a rather unusual but highly effective combination of a simultaneous application of a slight overpressure (25 mbar) at the injection end during the separation, and the use of a relatively narrow capillary of 50 microm inner diameter. Both measures would appear to be counterproductive. Optimisation of the probe concentration with regard to signal-to-noise ratio resulted in an electrolyte of 4 mM Orange G, 0.05% HPMC buffered at pH 7.7 by the addition of 10.0 mM histidine isoelectric buffer. Very high separation efficiencies of 128 000-297 000 plates were made possible by the relatively high probe concentration. Combined with excellent detection sensitivity, even with the introduction of hydrodynamic flow and a reduced optical path length, these measures resulted in limits of detection ranging from 0.216 to 0.912 microM with a deuterium lamp light source (248 nm) and from 0.147 to 0.834 microM with a 476 nm blue light-emitting diode (LED) light source. Reproducibility over 30 consecutive runs without changing the electrolyte was excellent, with relative standard deviation (RSD) values of 0.14-0.80% for migration time, 1.27-3.36% for peak area and 0.88-5.12% for peak heights. The optimised electrolyte was used for the analysis of inorganic anions in air filter samples, providing good agreement with results obtained by ion chromatography.

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Section: Analysis Of Air Filter Samplesmentioning

confidence: 98%

Sensitive indirect photometric detection of inorganic and small organic anions by capillary electrophoresis using Orange G as a probe ion

et al. 2003

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“…As can be seen from the table, the large majority of studies showed no comparison data. For the few that did, the comparison has been mainly between CZE and IC [3,23,25,41,43,45,63,65,67,70,72,74,76,77,84,86,96,97,104,106,110,111,113,119,124,126,127,131,134,135,140,141,146,148,149,152,158,174,190,194,195,219]. Typical examples include studies by Yang et al [126], who found that results obtained using a high-sensitivity method developed using sample stacking technique together with the use of an internal standard correlated well with a standard IC method, and work by Fung and Lau [149], who examined a CZE method for the analysis of inorganic anions in rainwater and used an IC technique in parallel, reporting that the results for the major anions were within statistical variation.…”

Section: Evaluating Accuracymentioning

confidence: 99%

Quantitative capillary zone electrophoresis of inorganic anions

Paull

King

2003

Electrophoresis

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Despite the availability of commercial capillary electrophoresis systems for over ten years, where quantitative analysis is required, capillary zone electrophoresis (CZE) has often failed to replace ion chromatography as the method of choice for a large number of analytes, not least inorganic anions. To investigate the reasons for this apparent failing, a review is presented of work that has been carried out to-date involving the quantitative application of CZE to the determination of inorganic anions in industrial and environmental samples. This review summarizes work both investigating and improving the quantitative aspects of the CZE of inorganic anions. A complete survey of how CZE has been applied to the determination of inorganic anions in real samples is given, including what, if any, analytical performance parameters were investigated and quoted, and if quality assurance data and validation methods were briefly considered, thoroughly investigated or simply ignored.

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“…As chromophore and buffer have been employed phtalate [11,15,18,62,67,72,73,91,98,100], benzoate [9,36,81,82,85,86], PDC [5,6,10,14,20,25,38,61,75], TMA [22,45,54,57,58,74], MES [16,31], PMA [36,37,41,82], 2,4-dihydroxybenzoic acid with e-aminocaproic acid [35,96], 1,2-dimethylimidazole and trimellitic acid [13], p-hydroxybenzoate [40,43,47,63], p-anisate [84], 3,5-dinitrobenzoic acid [42], salicylic acid with Tris [48,52], benzoic acid and Tris [107], NDC [39], ...…”

Section: Background Electrolytementioning

confidence: 99%

Capillary electrophoresis for short‐chain organic acids and inorganic anions in different samples

et al. 2003

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This review article is a comprehensive survey of capillary electrophoresis methods developed for the measurement of short-chain organic acids and inorganic anions in a wide variety of matrices, such as food and beverages, environmental, industry, and other applications, as well as clinical applications in body fluids such as urine, plasma or cerebrospinal fluid. Details of sample pretreatment and of electrophoretic conditions have been collected in tables, arranged by the type of matrix. Strategies employed for method development for the analysis of these compounds by capillary electrophoresis in real samples are discussed.

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Comparison of capillary zone electrophoresis with ion chromatography and standard photometric methods for the determination of inorganic anions in atmospheric aerosols

Cited by 30 publications

References 13 publications

Sensitive indirect photometric detection of inorganic and small organic anions by capillary electrophoresis using Orange G as a probe ion

Sensitive indirect photometric detection of inorganic and small organic anions by capillary electrophoresis using Orange G as a probe ion

Quantitative capillary zone electrophoresis of inorganic anions

Capillary electrophoresis for short‐chain organic acids and inorganic anions in different samples

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