Comparison of Cerenkov Luminescence Imaging (CLI) and gamma camera imaging for visualization of let-7 expression in lung adenocarcinoma A549 Cells

Improved radiosynthesis of 123I-MAPi, an auger theranostic agent

“…The crude mixture was purified by flash column chromatography (DCM:MeOH 0-10%) affording the title compound as a white solid (37.1 mg, 0.049 mmol, 29%); 1 H NMR (500 MHz, Chloroform-d) d 10.20 (bs, 1H), 8.46 (dt, J ¼ 6.9, 3.8 Hz, 1H), 7.79 À 7.67 (m, 3H), 7. 133.7, 131.8, 131.7, 129.5, 129.3, 128.4, 127.9, 127.3, 126.6, 125.0, 123.7 (d, J ¼ 17.8 Hz, 1 C), 116.3 (d, J ¼ 25.2 Hz, 1 C), 47.4 (2 C), 42.3 (2 C), 37.7, 29.0 (3 C), 27.3 (3 C), 13.7 (3 C), 9.6 (3 C); 19 F (Yang et al 2012 To a 1.5 mL microcentrifuge tube was added 4-(4-fluoro-3-(4-(3-(tributylstannyl)benzoyl)piperazine-1-carbonyl)benzyl) phthalazin-1(2H)-one (100 mg, 0.132 mmol) in anhydrous MeCN (10 mL), N-Chlorosuccinimide (NCS) (50 mg, 0.37 mmol) in a solution of anhydrous methanol (25 mL), acetic acid (10 mL), anhydrous acetonitrile (20 mL) and 123 I-NaI in NaOH 0.1 M (0.34-0.41 GBq, 9.29-11.04 mCi). After addition, the reaction was stirred at 650 rpm for 15 min at 25 C. The crude reaction was purified by RP-HPLC (C18 Waters Atlantis T3) column (C18-RP, 5 mm, 6 Â 250 mm) Solvent A: H 2 O, Solvent B: MeCN; flow rate: 1 mL/min; gradient: 0-5 min 5% B; 5-15 min 5-95% B; 15-18 min 95% B; 18-20 min 95 À 5% B).…”

Cerenkov luminescence imaging: physics principles and potential applications in biomedical sciences

“…Other preclinical CLI applications Potential uses of preclinical CLI which have been evaluated are also intraoperative imaging of radionuclides [109], surgical resection [110], endoscopic imaging for intraoperative surgical navigation [111], study of gene expression [57, 112–114], dynamic imaging [115], interscapular brown adipose tissue visualization [116], quantitive tissue oxygenation assessment [117], and negative contrast imaging of blood vessel attenuation of Cerenkov light to image vasculature [55]. In [118], Cerenkov-specific contrast agents for pH detection have been developed, while in [119] antibody-based PET radiotracers have been evaluated via CLI.…”

Cerenkov radiation: a multi-functional approach for biological sciences

“…However, combined high-resolution OI technique, CLI shows good prospect of 131 I imaging with high resolution. In another study, CLI was used for visualizing the expression of let-7 in lung adenocarcinoma A549 cells transfected with human sodium/iodine symporter-ras gene (hNIS-RU) [38]. Then, 3D CLI tomography was demonstrated to quantify tumor-associated aminopeptidase N (APN/CD13) expression with 131 I labeled Asn-Gly-Arg peptide ( 131 I-NGR) [39].…”