Comparison of chemical methods and immunoassay for the detection of pesticide residues in various matrices

Bonwick, Graham; Abdul‐Latif, P.; Sun, Cuilian; Baugh, P. J.; Smith, Christopher J.; Armitage, Ruth Ann; Davies, D. Huw

doi:10.1080/09540109409354838

Cited by 11 publications

(8 citation statements)

References 6 publications

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“…An immunoassay would provide a fast and selective method for the detection of FEN at trace levels 9–11. A number of enzyme‐linked immunosorbent assay (ELISA) methods have been reported for the detection of synthetic pyrethroids, such as permethrin,12–15 deltamethrin,16–18 cyhalothrin19 and flucythrinate 20…”

Section: Introductionmentioning

confidence: 99%

N‐Heterocyclic Carbene Catalyzed [4+2] Cycloaddition of Nitroalkenes with Oxodienes

Chen

Sun

2013

Chemistry A European J

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Section: Introductionmentioning

confidence: 99%

N‐Heterocyclic Carbene Catalyzed [4+2] Cycloaddition of Nitroalkenes with Oxodienes

Chen

Sun

2013

Chemistry A European J

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“…A number of enzymelinked immnosorbert assay (ELISA) methods have been reported for the detection of synthetic pyrethroids. They include allethrin (Pullen and Hock, 1995), s-bioallethrin (1R,3R,4′S-allethrin) (Wing et al, 1978), bioresmethrin (Hill et al, 1993), deltamethrin (Queffelec et al, 1998;Lee et al, 1998), fenpropathrin (Wengatz et al, 1998), and permethrin (Stanker et al, 1989;Skerritt et al, 1992;Bonwick et al, 1994). To our knowledge no study has been reported on the development of immunoassays for esfenvalerate.…”

Section: Introductionmentioning

confidence: 99%

Development of an Immunoassay for the Pyrethroid Insecticide Esfenvalerate

Shan

Stoutamire

Wengatz

et al. 1999

J. Agric. Food Chem.

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A competitive enzyme-linked immunosorbent assay was developed for the detection of the pyrethroid insecticide esfenvalerate. Two haptens containing amine or propanoic acid groups on the terminal aromatic ring of the fenvalerate molecule were synthesized and coupled to carrier proteins as immunogens. Five antisera were produced and screened against eight different coating antigens. The assay that had the least interference and was the most sensitive for esfenvalerate was optimized and characterized. The I(50) for esfenvalerate was 30 +/- 6.2 microg/L, and the lower detection limit (LDL) was 3.0 +/- 1.8 microg/L. The assay was very selective. Other pyrethroid analogues and esfenvalerate metabolites tested did not cross-react significantly in this assay. To increase the sensitivity of the overall method, a C(18) sorbent-based solid-phase extraction (SPE) was used for water matrix. With this SPE step, the LDL of the overall method for esfenvalerate was 0.1 microg/L in water samples.

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“…For example, one use for immunoassays would be to monitor the exposure of applicators and farm workers to pyrethroids, such as fenpropathrin, to reduce occupational exposure (Chen et al, 1991). Another possible application of immunoassays for pyrethroid residue analysis is the monitoring of food (Stanker et al, 1989;Skerritt et al, 1992;Hill et al, 1993) and environmental samples (Bonwick et al, 1994). The expected residues in food are generally low because of low application rates and relatively rapid degradation in the environment.…”

Section: Introductionmentioning

confidence: 99%

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of the Pyrethroid Insecticide Fenpropathrin

Wengatz

Stoutamire

Gee

et al. 1998

J. Agric. Food Chem.

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A competitive enzyme-linked immunosorbent assay (ELISA) was developed for the quantitative detection of fenpropathrin [(RS)-α-cyano-3-phenoxybenzyl-2,2,3,3-tetramethylcyclopropanecarboxylate]. Polyclonal antisera were isolated from rabbits immunized with two different fenpropathrin hapten conjugates. One hapten contained an amino function; the other contained a carboxyl group for conjugation to carrier proteins. Mollusk hemocyanins, thyroglobulin, and fetuin were used as carrier proteins. The antisera varied greatly in their affinities for fenpropathrin. A homologous assay system using the coating antigen format was the most sensitive. The IC50 for fenpropathrin was 20 μg/L, and the lower detection limit was 2.5 μg/L. Pyrethroids, such as phenothrin, permethrin, resmethrin, fenvalerate, deltamethrin, cyfluthrin, and cypermethrin, and the pyrethroid metabolites, 3-phenoxybenzoic acid and fenpropathrin acid, did not cross-react significantly in this assay. Ten percent acetone or methanol and a pH of 4 were determined to be optimum assay conditions. Various cationic, anionic, and nonionic detergents had no significant effect on the assay. Keywords: Fenpropathrin; pyrethroid; ELISA; pesticide; enzyme immunoassay; cross-reactivity

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Comparison of chemical methods and immunoassay for the detection of pesticide residues in various matrices

Cited by 11 publications

References 6 publications

N‐Heterocyclic Carbene Catalyzed [4+2] Cycloaddition of Nitroalkenes with Oxodienes

N‐Heterocyclic Carbene Catalyzed [4+2] Cycloaddition of Nitroalkenes with Oxodienes

Development of an Immunoassay for the Pyrethroid Insecticide Esfenvalerate

Development of an Enzyme-Linked Immunosorbent Assay for the Detection of the Pyrethroid Insecticide Fenpropathrin

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