Comparison of Chicken Immune Responses to Immunization with Vaccine La Sota or ZG1999HDS Strain of Newcastle Disease Virus

Abstract

Newcastle disease (ND) is a highly contagious avian disease. Global control of ND is mainly based on vaccination of poultry; however, reported outbreaks of ND in vaccinated flocks indicate a constant need to re-evaluate the existing vaccines and a development of the new ones. In this study, 4-week-old male chickens of the layer commercial hybrid were immunized oculonasally with a commercial NDV live La Sota vaccine (LS group), a suspension of lyophilized NDV strain ZG1999HDS (ZG group), or saline (Control (K) group). Antibody response was determined by haemagglutination inhibition (HI) assay. Cell-mediated immunity (CMI) was characterized by immunophenotyping of leukocyte’s and T-lymphocyte’s subpopulations (flow cytometry). Applied NDV strains did not cause any adverse reaction in treated chickens. Both strains induced the significantly higher HI antibody response in comparison to the control group, and overall antibody titer was higher in ZG group than in LS group. CMI, manifested as a higher proliferation of B- and T-helper cells, yielded better results in the ZG groups than in the LS group. Based on the obtained results, we conclude that the strain ZG1999HDS is immunogenic and is a suitable candidate for further research and development of poultry vaccines.

Evaluation of the immune response of layer chickens to Newcastle disease virus vaccines using the new vaccination regimens

“…(2020). Administering a double dose of vaccination to a flock of chickens addresses the variability in immune responses, ensuring that even birds with weaker immune systems can develop protective immunity (Nedeljkovic et al., 2022; Illango et al., 2005; Sarba et al., 2021). This finding also supports previous study (Hu et al., 2022; Dimitrov et al., 2017; Miller et al., 2013; Ravikumar et al., 2022), which found that a double dose offers a higher concentration of antigens, boosting the possibility of a stronger and more effective immune response in chickens.…”

Oncolytic activity of Newcastle disease virus strain zg1999hds on oral malignant melanoma in dog: A case report

“…This Case Report reports on the use of NDV strain ZG1999HDS as an oncolytic agent in the treatment of OMM in a dog. The strain was reported to be mild, superior to La Sota strain of NDV as immunogenic and cytolitic agent for several tumour cells lines in vitro (FsaR -mouse fibrosarcoma, SC-CVII-mouse squamous cell carcinoma, CT26WTmouse colon adenocarcinoma, 4T1 -mouse breast adenocarcinoma) and in vivo (CT26WT and 4T1) (Nedeljković et al, 2022;Mazija et al, 2011).…”

Molecular characterization of Newcastle disease virus vaccines in Nigeria

“…Biosecurity involves various techniques, such as cleaning, disinfecting, preventing farm access by rodents and wild birds, and strict hygiene practices among visitors and farm staff [ 20 ]. In chickens vaccinated with a combination of live and inactivated vaccines, a strong level of immunity is exhibited compared with that in chickens receiving only live vaccines; meanwhile, genotype-matched vaccines provide better protective immunity than heterogeneous vaccine strains [ 21 ]. Live LaSota and B1 vaccines were first developed based on lentogenic NDV strains and are the vaccine strains that are most commonly used worldwide [ 22 ].…”