2015
DOI: 10.1002/jmv.24206
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Comparison of clinical performances among Roche Cobas HPV, RFMP HPV PapilloTyper and Hybrid Capture 2 assays for detection of high-risk types of human papillomavirus

Abstract: The cervical cancer screening guidelines suggest that early detection of HPV16 and HPV18 is helpful for identifying women with cervical intraepithelial neoplasia (CIN) grade two or higher. We comparatively evaluated three HPV DNA assays, Roche Cobas HPV, RFMP HPV PapilloTyper, and Hybrid Capture 2 (HC2). A total of 861 cervical swab samples from women over 30 years of age were classified into two groups, that is, high grade squamous intraepithelial lesion (HSIL) and non-HSIL, according to cervical cytology res… Show more

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Cited by 5 publications
(1 citation statement)
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“…Currently, the way to test for HPV6 and HPV11, including genotype specific polymerase chain reaction (PCR), Quantitative real-time PCR (qPCR), polymerase chain reaction-restricted fragment length polymorphisms (PCR-RELP), droplet digital PCR (ddPCR), loop-mediated isothermal amplification (LAMP) and hybrid capture 2 test (HC2) ( Table 1 ). these kits possess high specificity and sensitivity, but require high skilled technical personnel, high cost, long detection time, well-equipped laboratories, cumbersome temperature-control devices and sophisticated analysis, which limit the wide adoption of these methods in resource-poor areas ( Grce et al., 2000 ; Hawkins et al., 2013 ; Lindh et al., 1992 ; Oliveira et al., 1994 ; Yu et al., 2015 ). Therefore, it is essential to develop a more efficient method for rapid screening of HPV6 and HPV11 infections to promote early detection and treatment.…”
Section: Introductionmentioning
confidence: 99%
“…Currently, the way to test for HPV6 and HPV11, including genotype specific polymerase chain reaction (PCR), Quantitative real-time PCR (qPCR), polymerase chain reaction-restricted fragment length polymorphisms (PCR-RELP), droplet digital PCR (ddPCR), loop-mediated isothermal amplification (LAMP) and hybrid capture 2 test (HC2) ( Table 1 ). these kits possess high specificity and sensitivity, but require high skilled technical personnel, high cost, long detection time, well-equipped laboratories, cumbersome temperature-control devices and sophisticated analysis, which limit the wide adoption of these methods in resource-poor areas ( Grce et al., 2000 ; Hawkins et al., 2013 ; Lindh et al., 1992 ; Oliveira et al., 1994 ; Yu et al., 2015 ). Therefore, it is essential to develop a more efficient method for rapid screening of HPV6 and HPV11 infections to promote early detection and treatment.…”
Section: Introductionmentioning
confidence: 99%