Abstract:The aim of this study was to compare bone density of bone defects treated with lyophilizated amniotic membrane (LAM) and collagen membrane (CM) at three and five weeks. Two bone defects of 4mm in diameter and 6mm deep were created in left distal femoral diaphysis of New Zealand rabbits (n=12). The animals were randomly divided into two groups. One of the defects was covered with lyophilized amniotic membrane (Rosa Chambergo Tissue Bank/National Institute of Child Health-IPEN, Lima, Peru) or collagen membrane (Dentium Co, Seoul, Korea). The second one was left uncovered (NC). The rabbits were killed after three and five weeks (three rabbits/period). The results showed a high bone density and defect repair by new bone. The tomographic study revealed that bone density of the defects treated with LAM at three weeks was equivalent to the density obtained with CM and higher density compared with NC (p<0.05). At five weeks, the bone density obtained with LAM was more than the density obtained with CM and NC (p<0.05). The histomorphometric study showed no significant differences between LAM and CM at three and five weeks (p>0.05). The results show that the lyophilizated amniotic membrane provides equal or higher bone density than the collagen membrane.