Comparison of COBE� Spectra? software version 4.7 PBSC and version 6.0 auto PBSC? program

Wilke, Robert; Brettell, M; Prince, H.M.; Wolf, M.; McKenna, Robert W.; Serpell, E; Molloy, Mick; Stevens, Hallam; Quinn, Michael; Gates, Priscilla; Chapple, J. Paul

doi:10.1002/(sici)1098-1101(1999)14:1<26::aid-jca5>3.0.co;2-1

Cited by 17 publications

(15 citation statements)

References 4 publications

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“…The most remarkable difference between the two procedures was that products collected by using the AutoPBSC procedure were contaminated with fewer platelets than products obtained by using the MNC. This finding was previously reported in leukapheresis performed for allogenic PBSC collection in mobilized donors and patients receiving autologous PBSC separation or photopheresis [16,17,22,23].…”

Section: Discussionsupporting

confidence: 83%

Leukapheresis for the extraction of monocytes and various lymphocyte subpopulations from peripheral blood: product quality and prediction of the yield using different harvest procedures

Wolf¹,

Meyer²,

Riggert³

2005

Vox Sanguinis

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Section: Discussionsupporting

confidence: 83%

Leukapheresis for the extraction of monocytes and various lymphocyte subpopulations from peripheral blood: product quality and prediction of the yield using different harvest procedures

Wolf¹,

Meyer²,

Riggert³

2005

Vox Sanguinis

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“…If the PB CD34 ϩ cells exceeded 5 ϫ 10 6 /l on the morning of the planned collection, apheresis was performed on that day. Indeed, we have previously demonstrated the utility of this threshold value for initiating apheresis 17,20 and the strong correlation between PB CD34 ϩ cell and the autograft CD34 ϩ cell content was confirmed in this study (Spearman correlation; r = 0.8643, P Ͻ 0.0001) (Figure 1). The peak days for PB CD34 ϩ cells were day 8 and day 9.…”

Section: Apheresis Collectionssupporting

confidence: 78%

“…Of the total 72 initial sets of collections performed, the median number of CD34 ϩ cells collected was 3.4 ϫ 10 Given the recognised early neutrophil nadir with docetaxel, 12 we were particularly interested in examining the kinetics of CD34 ϩ cell mobilisation following docetaxel and G-CSF. Having previously demonstrated the value of a morning PB CD34 count in predicting PBPC yield 17,20 we found that harvesting was required in some patients as early as day 7 (median time to initiate collection was day 8) with all patients commencing collection between days 7 and 9. In comparison, when we mobilized a similar cohort of patients with ifosfamide with either doxorubicin or paclitaxel with the same G-CSF regimen, the peak days for collection were later (days [10][11][12].…”

Section: Discussionmentioning

confidence: 78%

Docetaxel effectively mobilizes peripheral blood CD34+ cells

Prince

Toner

Seymour

et al. 2000

Bone Marrow Transplant

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Summary:We prospectively evaluated docetaxel (100 mg/m 2 ) with G-CSF (10 g/kg S.C., daily) for mobilization efficiency in 26 patients with breast cancer. The minimum target yield was Ͼ4.5 ؋ 10 6 CD34 ؉ cells/kg (optimum = 9 ؋ 10 6 /kg), sufficient to support the subsequent three cycles of high-dose therapy (HDT). The peak days for peripheral blood (PB) CD34؉ cells were day 8 and day 9. Seven collections began on day 7, 16 on day 8 and three on day 9. The median peripheral blood progenitor cell (

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“…Nevertheless, this change in the procedure could influence other separation parameters. Concerning our data (Table 1a) and also some data in the literature, 24,25 it seems that harvests using the autoPBSC program, albeit causing less thrombocytopenia, might also be less effective for CD34 þ collection.…”

Section: Discussionsupporting

confidence: 55%

Mathematical model of peripheral blood stem cell harvest kinetics

Mayer

Pospíšil

Korístek

2003

Bone Marrow Transplant

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Summary:A mathematical model of peripheral blood stem cell harvests was developed, taking two new parameters R (number of recruited cells/minute) and E f (efficiency of collection) into consideration in addition to concentrations and collected amounts of cells. This model was tested on 241 harvest procedures in cancer patients (chemotherapy þ G-CSF stimulation), donors of allogeneic PBSC, and platelet donors, using different collection procedures, with a Cobe Spectra Cell separator. The relationships between preapheresis concentrations, R, E f and harvested amounts of cells were complex, and different for different harvest procedures and populations of donors. However, invariably, recruitment played an important role and contributed significantly to the final harvest in all types of cells studied. For example, for the patient group, mean recruitment was 1.3 Â 10 6 CD34 þ cells/min and the amount of recruited cells corresponded to 65% of all collected cells. Recruitment was significantly influenced by pretreatment with chemo-therapy and/or radiotherapy. The mean recruitment values for the subgroups with limited, moderate, and extensive pretreatment were 1.65 Â 10 6 , 0.87 Â 10 6 , and 0.32 Â 10 6 CD34 þ cells released per minute, respectively. The finding of a quick and massive recruitment phenomenon may stimulate further research into hematopoiesis in order to maximize harvested cells.

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Comparison of COBE� Spectra? software version 4.7 PBSC and version 6.0 auto PBSC? program

Cited by 17 publications

References 4 publications

Leukapheresis for the extraction of monocytes and various lymphocyte subpopulations from peripheral blood: product quality and prediction of the yield using different harvest procedures

Leukapheresis for the extraction of monocytes and various lymphocyte subpopulations from peripheral blood: product quality and prediction of the yield using different harvest procedures

Docetaxel effectively mobilizes peripheral blood CD34+ cells

Mathematical model of peripheral blood stem cell harvest kinetics

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