Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: Anin vitro Cell Culture Study

Abstract

Aim: The aim of this study is to assess and compare the efficacy of Jordanian propolis and full concentration mature coconut water in their ability to preserve periodontal ligament (PDL) cell viability after exposure of PDL cells to up to 120 minutes dry storage.Materials and methods: PDL cells were obtained from sound permanent first molars which were cultured in Dulbecco's Modified Eagles Medium (DMEM). Cultures were subjected to 0, 30, 45, 60, 90 and 120 minutes dry storage times then incubated with 100% mature coconut water, Jordanian propolis and DMEM for 45 minutes at room temperature (18-26°C). Untreated cells served as controls at each dry storage time tested. PDL cell viability was assessed by MTT assay. Statistical analysis of data was accomplished by using one-way analysis of variance complemented by Tukey test and the level of significance was 5% ( p < 0.05).Results: Up to 60 minutes dry storage, no significant improvement on the percentage of viable cells was found from soaking in all tested media. On the other hand, soaking in mature coconut water only resulted in higher percentages of viable cells at >60 minutes dry storage. However, this improvement was not significant (p > 0.05).Conclusion: Avulsed teeth which have been left dry for <45 minutes should be replanted immediately, whereas avulsed teeth which have been left dry for >45 minutes may benefit from soaking for 45 minutes in mature coconut water.How to cite this article: Al-Haj Ali SN, Al-Jundi S, Mhaidat N. Comparison of Coconut Water and Jordanian Propolis on Survival of Bench-dried Periodontal Ligament Cells: An in vitro Cell Culture Study. Int J Clin Pediatr Dent 2013;6(3):161-165.

Effect of two different storage media of avulsed tooth on viability and apoptosis of human gingival fibroblast: An in vitro study.

“…Avulsion of permanent tooth is considered the most serious traumatic injury resulting in aesthetic, functional and psychological problems to injured patient (37,38) . The recommended treatment in such case is instant replantation of the dislodged tooth; however, it is not always possible to do so.…”

Medios De Conservación De Las Piezas Avulsionadas: Revisión De Literatura Basada en Evidencia

“…Contiene aminoácidos, oligoelementos, vitaminas, minerales y aceites esenciales Gracias a la acción antibiótica del propóleo, que protege de la actividad de virus y bacterias, la colmena es uno de los lugares más estériles conocidos en la naturaleza. No obstante, estudios científicos han demostrado que el efecto del propóleo se consigue gracias a la acción sinérgica de todos sus componentes y concluyen que es tan efectivo como la leche y el HBSS (18,27,30,31,34). Leche: En 1981 Andreasen (Dinamarca) y Blomlof, Lindskog y Hammarstrom (Suecia) fueron los primeros en reportar sobre la viabilidad de la leche fresca descremada pasteurizada como medio para conservar un diente avulsionado.…”

“…Ahangari Z en su artículo del 2013 concluye que hay una alta tasa de éxito que se puede conseguir cuando los dientes avulsionados se mantienen en condiciones húmedas y llevados a una clínica dental tan pronto como sea posible (31). EN 2013 Al-Haj Ali SN en un artículo y de Paula Reis MV en otro publican que el Aloe Vera al 10, 30 y 50% puede ser recomendado como medio de almacenamiento celular (27,28). En el mismo artículo del 2013 de Paula Reis MV propone el agua de coco y la leche de soja como medios de almacenamiento debido a sus nutrientes las cuales conservan la viabilidad celular El agua de coco resultaría ser un medio de almacenamiento prometedor para los dientes avulsionados Los estudios indicaron que el grupo de agua de coco demostró un número significativamente mayor de células PDL viable que el propoleo (28,30).…”

Natural products in endodontics

“…The results showed that avulsed teeth, which have been left dry for more than 45 minutes, soaked in mature coconut water for 45 minutes in mature coconut water could be beneficial. 31 Prueksakorn et al 32 examined the preservative and proliferative effects of Thai propolis extract. Their results exhibited that 2.5 mg ml-1 of Thai propolis was the appropriate dose for preserving the viability of PDL cells, and it was comparable to Hanks Balanced Salt Solution (HBSS).…”