Comparison of colorimetric and chemiluminescent enzyme‐linked immunosorbent assay for the detection of endosulfan in food samples

Abstract

Pesticides have become part of food protection since their inception. Endosulfan, an organochlorine insecticide, has been used against insect pests such as whiteflies, aphids, red spiders and mites. Methods of immunochemical assays have been devised for the determination and analysis of pesticides and commonly used for the analysis of contaminants in food, water, soil and body fluids. Chicken IgY antibodies raised against endosulfan haptens were used for the detection of endosulfan. We have compared colorimetric (CO) and chemiluminescence (CL) enzyme-linked immunosorbent assay (ELISA) techniques for the detection of endosulfan isomers in a food matrix. CL ELISA assay was found to be more sensitive than CO assay. The mean recovery was 81.2-95.6% for α- and β-endosulfan-spiked food samples with 2.8-4.6% relative standard deviation. The detection of the endosulfan isomers was linear in the range 100 µg/mL-5 fg/mL, with a limit of detection at 100 µg/mL and 5 fg/mL for the CL ELISA method and 100 µg/mL and 1 ng/mL for the CO ELISA method respectively. These methods can be used for the rapid and reliable detection of organochlorine pesticide endosulfan.

Advances in Chicken IgY-Based Immunoassays for the Detection of Chemical and Biological Hazards in Food Samples

“…In addition, colorimetric and chemiluminescent ELISAs based on IgY have been applied for detecting endosulfan. 148 Endosulfan is a 7:3 mixture of α and β stereoisomers. Therefore, IgY against endosulfan isomers (both α and β) was used.…”

“…In addition, the applicability of the immunoassay was proven by detecting glyphosate in surface water with a recovery of 86–135%. In addition, colorimetric and chemiluminescent ELISAs based on IgY have been applied for detecting endosulfan . Endosulfan is a 7:3 mixture of α and β stereoisomers.…”