2016
DOI: 10.1016/j.smallrumres.2015.12.007
|View full text |Cite
|
Sign up to set email alerts
|

Comparison of commercial Triladyl extender with a tris-fructose-egg-yolk extender on the quality of frozen semen and pregnancy rate after transcervical AI in Bangladeshi indigenous sheep (Ovis aries)

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

1
15
0

Year Published

2016
2016
2022
2022

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 15 publications
(16 citation statements)
references
References 21 publications
1
15
0
Order By: Relevance
“…Sheep insemination pipette with an excentrical tip (Minitube, Germany) with 0.25 ml semen straw was introduced in to the cervix and tried to push forward by manipulating through the cervical rings. Semen was then expelled from the pipette as deeply as possible in the cervix (Kumar and Naqvi, 2014;Rekha et al, 2016).…”
Section: Transcervical Ai In Indigenous Ewesmentioning
confidence: 99%
“…Sheep insemination pipette with an excentrical tip (Minitube, Germany) with 0.25 ml semen straw was introduced in to the cervix and tried to push forward by manipulating through the cervical rings. Semen was then expelled from the pipette as deeply as possible in the cervix (Kumar and Naqvi, 2014;Rekha et al, 2016).…”
Section: Transcervical Ai In Indigenous Ewesmentioning
confidence: 99%
“…Functional membrane integrity (FMI). FMI was evaluated by the hypoosmotic test (HOST) (Rekha et al, 2016). For this, 50 μL of thawed semen was mixed with 200 μL of a prewarmed (37 °C) hypoosmotic solution of 5.4% sucrose (100 mOsmol/l) in Falcon tubes, and incubated at 37 °C for minutes.…”
Section: Structural Membrane Integrity (Smi)mentioning
confidence: 99%
“…Usually, during freezing-thawing process of semen, the sperm undergoes several vital biological and functional changes due to cold shocks and cryo-injuries, especially affects membranes that distresses their functional abilities (Nur et al, 2010;Chelucci et al, 2015). To establish a simple and sustainable cryopreservation protocol for ram semen in Bangladesh, efforts are focused on different types of diluents, dilution and freezing methods (Mahmuda et al, 2015;Rekha et al, 2016;Rekha et al, 2018;Jha et al, 2019). Evidence from previous studies, a hand-made tris-fructose-citrate (20% egg yolk and 5-7% glycerol) and commercial diluents Triladyl ® , two-steps dilution (fractional procedure) and three-steps manual freezing (in LN2 vapourusing styrofoam) are seemed to be recommended protocol for freezing ram semen in Bangladesh, already extended for field fertility testing (Rekha et al 2016;Rekha et al 2018;Jha et al, 2019).…”
Section: Introductionmentioning
confidence: 99%
“…To establish a simple and sustainable cryopreservation protocol for ram semen in Bangladesh, efforts are focused on different types of diluents, dilution and freezing methods (Mahmuda et al, 2015;Rekha et al, 2016;Rekha et al, 2018;Jha et al, 2019). Evidence from previous studies, a hand-made tris-fructose-citrate (20% egg yolk and 5-7% glycerol) and commercial diluents Triladyl ® , two-steps dilution (fractional procedure) and three-steps manual freezing (in LN2 vapourusing styrofoam) are seemed to be recommended protocol for freezing ram semen in Bangladesh, already extended for field fertility testing (Rekha et al 2016;Rekha et al 2018;Jha et al, 2019). However, more effort needs to standardize and select a sustainable frozen ram semen production technology, both in terms of dilutes and freezing methods.…”
Section: Introductionmentioning
confidence: 99%