Comparison of commercially available media for detection and isolation of Ureaplasma urealyticum and Mycoplasma hominis

Abstract

The Mycotrim Triphasic flask system (Irvine Scientific, Irvine, Calif.) was compared with a system composed of Mycotrim GU broth (Irvine Scientific) and A7 or A8 agar (Remel, Lenexa, Kans.) for the ability to detect Ureapla*ma urealyticum and Mycoplasma hominis from 129 genital specimens. Of the 64 specimens positive for U. urealyticum, 25, 98, and 100%o were detected on Mycotrim Triphasic agar and A7 and A8 agars, respectively. All 18 specimens that grew M. hominis were detected by A7 and A8 agars, and 94% grew on Mycotrim Triphasic agar. Mycotrim GU broth detected all of the positive specimens, and Mycotrim Triphasic broth detected all but one. Mycotrim GU broth inoculated simultaneously with either A7 or A8 agar was found to be more sensitive and cost-effective than the Mycotrim Triphasic flask system.

Evaluation of the MYCOPLASMA IST3 urogenital mycoplasma assay in an international multicentre trial

“…Species identification by the MYCOPLASMA IST3 was compared with A7 agar identification by colony morphology 18 for M. hominis (‘fried egg’, i.e. central colony with lower surrounding coronal dispersion across the surface of the agar) and Ureaplasma species (‘sea urchin’, i.e.…”

“…MICs were determined for all isolates using broth microdilution methods compliant with CLSI M43A guidelines (thresholds detailed in Supplementary Materials and methods ). 10 , 11 , 17 Confirmation of species was performed by colony morphology determination on A7 agar 18 and by specific multiplex qPCR relative to a standardized plasmid containing defined copies of all primer/probe targets as previously published. 15 Viability and quantification of M. hominis and Ureaplasma spp.…”

A New Multiplex Genetic Detection Assay Method for the Rapid Semi-Quantitative Detection of Six Common Curable Sexually Transmitted Pathogens From the Genital Tract

“…These pathogens cannot be grown using normal microbiological culture methods, except N. gonorrhoeae which is fastidious and often will also be culture negative but PCR positive. Molecular diagnostic methods for gene detection technology, such as polymerase chain reaction (PCR) and high-throughput sequencing are useful for the identification of microorganisms that are difficult to culture due to their slow growth, or because of complex procedural issues, or the need specific transport protocols and culture media ( Broitman et al, 1992 ). However, high-throughput sequencing is expensive and is not recommended for the routine screening of these pathogens.…”