Comparison of commercially available New York City medium and Martin-Lewis medium for recovery of Neisseria gonorrhoeae from clinical specimens

Lawton, William D.; Koch, Lori

doi:10.1128/jcm.16.4.754-755.1982

J Clin Microbiol

1982

DOI: 10.1128/jcm.16.4.754-755.1982

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Comparison of commercially available New York City medium and Martin-Lewis medium for recovery of Neisseria gonorrhoeae from clinical specimens

William D. Lawton¹,

Lori Koch²

Abstract: In 100 clinical specimens containing Neisseria gonorrhoeae, commercially prepared New York City medium in JEMBEC plates was superior to Martin-Lewis medium for recovery of gonococci in 49 specimens, equal to Martin-Lewis medium in 43 specimens, and less effective in 8 specimens.

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An evaluation of pre-poured selective media for the isolation of Neisseria gonorrhoeae

Young¹,

Moyes²

1996

Journal of Medical Microbiology

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Fourteen commercial media supplied as pre-poured plates were compared with an 'inhouse' selective medium for their ability to support the growth of 105 gonococcal isolates (representing a wide variety of serovars encountered in natural infection), 25 meningococcal and 20 Neisseria lactamica isolates, and to inhibit the growth of 71 isolates of non-pathogenic neisseriae and miscellaneous organisms. Only two of the prepoured plate media and the in-house selective medium yielded growth of duplicate cultures of all 105 gonococcal isolates after incubation for 24 h: one other medium provided growth of all the isolates after incubation for 48 h. The ability of the various media to suppress the growth of the 71 isolates of non-pathogenic neisseriae and miscellaneous organisms ranged from 97.2 to 71.8% of isolates inhibited. Of the four media that enabled growth of all the gonococcal strains, inhibition was 94.4% for the inhouse medium, 85.9% and 80.3% for the two media on which all gonococci grew after 24 h and 71.8% for the medium on which all of the gonococci grew after 48 h. Failure of growth of gonococci was associated with: serogroup IA isolates (p < 0.001), AHU auxotype (p < 0.001) and the presence of vancomycin rather than lincomycin in the selective medium (p < 0.02).

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An evaluation of pre-poured selective media for the isolation of Neisseria gonorrhoeae

Young¹,

Moyes²

1996

Journal of Medical Microbiology

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Comparative evaluation of New York City and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

Greenwood¹,

Voß²,

Smith³

et al. 1986

J Clin Microbiol

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Commercially manufactured New York City (NYC) medium and modified Thayer-Martin (MTM) medium were compared for their ability to isolate Neisseria gonorrhoeae from clinical specimens. Twenty-seven public health laboratories throughout California evaluated 4,802 specimens collected from patients attending either sexually transmitted disease or family planning clinics. Totals of 726 and 737 N. gonorrhoeae isolates were recovered from NYC and MTM medium, respectively. Although less contamination was noted on NYC medium, MTM medium was equivalent to commercially prepared NYC medium for the isolation of N. gonorrhoeae from clinical specimens.

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Comparison of commercially available New York City medium and Martin-Lewis medium for recovery of Neisseria gonorrhoeae from clinical specimens

Abstract: In 100 clinical specimens containing Neisseria gonorrhoeae, commercially prepared New York City medium in JEMBEC plates was superior to Martin-Lewis medium for recovery of gonococci in 49 specimens, equal to Martin-Lewis medium in 43 specimens, and less effective in 8 specimens.

Cited by 2 publications

References 2 publications

An evaluation of pre-poured selective media for the isolation of Neisseria gonorrhoeae

An evaluation of pre-poured selective media for the isolation of Neisseria gonorrhoeae

Comparative evaluation of New York City and modified Thayer-Martin media for isolation of Neisseria gonorrhoeae

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