Comparison of Conventional and Commercial Trichrome Staining Methods for Detecting Protozoan in Stool Samples

Aydın, Mesut; Adıyaman, G.; Kaya, Tayfun; Kuştımur, Semra; Al, Funda Doğruman

doi:10.9775/kvfd.2012.6089

Cited by 3 publications

(4 citation statements)

References 3 publications

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“…They also stated that, because direct microscopy has a limited sensitivity, antigen detection procedures such as ELISA should be used to confirm diagnosis in individuals with suspected amoebiasis [36]. According to a 2012 study, persistent trichrome staining is the preferred approach of E.histolytica detection since it enables for later examination of faeces for identification of the protozoa's internal structure [37]. In 2014, researchers discovered that stool antigen testing by ELISA for the diagnosis of amoebiasis has 100% sensitivity and 100% specificity, and they advised that this technology be used as a diagnostic test [38].…”

Section: Discussionmentioning

confidence: 99%

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

Onosakponome¹,

Ohanu²,

U.³

2021

MRJI

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Objective: Amoebiasis is a parasitic infection caused by E.histolytica, accurate diagnosis of E. histolytica is important in the treatment of amoebiasis and to avoid preventable costs. The study objective is to compare different diagnostic methods used in the diagnosis of amoebiasis for detection of E.histolytica parasite. Materials and Methods: Faecal and serum specimens of 200 patients defined as symptomatic (diarrhea and dysentery) and asymptomatic (a case history of E.histolytica infection) was used for the study .Stool specimen was examined with microscopy, cultured in Boeck and drbolav’s medium and anti-E. histolytica antibodies were investigated using a latex slide test. Stool samples were also examined by immunoassay methods for specific antigens which is the reference standard for comparison. Result: Two hundred (200) samples examined for E.histolytica parasite 12(6.0%) were positive in in microscopy,34(17%) in bichro-latex antibody test and 6(3.0%) in Boeck and drbolav’s culture medium. The three test methods showed significant detection of E.histolytica parasite(p<0.05). Microscropic method detected 100% of E.histolytica infection in symptomatic patients and Boeck and Drbohlav’s culture medium detected 33.3%. However, the method of diagnosis is not associated with the diagnosis E.histolytica infection in asymptomatic and symptomatic Patients(p>0.05).The diagnostic precision of the microscopy diagnostic method showed that sensitivity was 40.2%, specificity was 82.3%, PPV 39.6% and NPV 70.4% .The sensitivity was 86.6%, specificity was 70.6% PPV 87.6% and NPV 75.6% for bichro-latex antibody test and The sensitivity was 20.6 %, specificity was 50.6 %, PPV 34.6%, NPV 61.2% for Boeck and Drbohlav’s culture medium Conclusion: The result from the comparison of the three diagnostic methods for E.histolytica parasite indicate high sensitivity and specificity for bichro-latex antibody test when compared with the other methods. however, in areas were molecular technology such as Polymerase chain reaction and enzyme linked immunosorbent assay is not available, bichro-latex antibody assay is recommended. although,microscopic examination can be used in diagnosis of amoebiasis in geographical areas with technological limitation in health.

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Section: Discussionmentioning

confidence: 99%

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

Onosakponome¹,

Ohanu²,

U.³

2021

MRJI

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“…They also reported that, due to the low sensitivity of direct microscopy, the use of antigen detection methods by ELISA would be appropriate to confirm diagnosis in patients with suspected amoebiasis. Aydin et al [22] stated that the preferred method is permanent trichrome staining because it allows faeces to be examined later for the identification of the internal structure of the protozoa.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Various Methods in the Diagnosis of Entamoeba histolytica in Stool and Serum Specimens

2016

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Objective: Entamoeba histolytica is indistinguishable from Entamoeba dispar in direct microscopic examination. A definitive diagnosis of E. histolytica is important in terms of the treatment of the patient and to avoid unnecessary costs. This study's aim is to determine the prevalence of E. histolytica and to make a comparison of the different diagnostic tests in the patients specimens defined as E. histolytica/E. dispar infection. Materials and Methods:Faecal and serum specimens of 90 patients defined as E. histolytica/E. dispar with microscopy (wet mount examination with 0.85% saline and Lugol's iodine) were examined. Stool samples were examined by trichrome staining for trophozoites and cysts and by immunoassay methods for specific adhesin antigens (Wampole ® E. histolytica II antigen testing) and for specific serine-rich 30 kD membrane protein (Serazym® E. histolytica antigen testing). Anti-E. histolytica antibodies were investigated using a latex slide test and indirect hemagglutination methods in serum specimens. Results:Presence of E. histolytica was not confirmed in 31.1% cases with trichrome staining, 62.2% of the Wampole antigen test, 64.4%, of the Serazym antigen test, 73.3% of the indirect hemagglutination test and 75.6%. of the latex agglutination. Considering the common results from Wampole and Serazym antigen testing as a reference standard, the specificity/sensitivity is 100/53.85% for trichrome staining, 75.00/98.11% for the latex agglutination test and 78.57/96.77% for the indirect hemagglutination test. Conclusion:It has been shown that investigation of E. histolytica in stools by direct wet-smear microscopy alone can cause significant false positive results. To obtain a reliable diagnosis for E. histolytica and to avoid unnecessary treatment for this parasite, at least one more specific assay, particularly an antigen testing and microscopy, is required. Gereç ve Yöntem: Nativ incelemeyle (dışkı örnekleri serum fizyolojik ve lügolde süspansiye edilerek) E. histolytica/E. dispar tanısı konulmuş 90 olgunun dışkı ve serum örnekleri incelenmiştir. Dış-kı örnekleri; trikrom boyama ile direkt mikroskobik olarak; özgün adezin antijeni Wampole® E. histolytica II kitleriyle ve spesifik antijen Serin-rich 30kD membran proteinleri Serazym® E. histolytica (Seramun Diagnostic Gmbh, Almanya) kitleriyle immunoassay yöntemiyle incelenmiştir. Serum örneklerinde anti-E. histolytica antikorları lateks lam testi ve indirekt hemaglütinasyon yönte-miyle araştırılmıştır. KeywordsBulgular: E. histolytica tanısı trikrom boyama yöntemiyle olguların %31,1'inde, Wampole antijen testiyle %62,2'sinde, Serazym antijen testiyle %64,4'ünde, indirekt hemaglütinasyon testiyle %73,3'ünde ve lateks lam aglütinasyon testiyle %75,6'sında ör-nekte doğrulanmamıştır. Wampole antijen testi ve Serazym antijen testi ortak sonuçları referans alındığında testlerin duyarlılık/ özgüllükleri sırasıyla trikrom boyama için %100/53,85, lateks aglütinasyonu için %75,00/98,11 ve indirekt hemaglütinasyon testi için %78,57/96,77 olarak bulun...

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“…Trikrom boyama dışında demir hematoksilen, giemsa, field ve wright boyaları da başarıyla kullanıl-maktadır. Kültür yöntemi Blastocystis tanısında duyarlılığı yüksek bir yöntemdir ancak zaman alıcı bir yöntem olup, sonuçların en az 2-3 gün beklenmesi ve bazı Blastocystis izolatlarının kültürde çoğalmaması gibi zorlukları bulunmaktadır (1,3,(12)(13)(14)(15) .…”

Section: Introductionunclassified

“…Kuruyan lamların üzerine kapatıcı (Eukitt ® Quick-hardening mounting medium, Fluka Biochemika, ABD) damlatılarak lamelle kapatıldı. Preparatlar immersiyon yağı kullanılarak ışık mikroskobunda 100x objektifte incelendi (15) .…”

Section: Introductionunclassified

A Comparison of a Modified Formalin-Ether Concentration Technique with a Commercial (Feconomics®) Method and Its Two Modifications in the Investigation of Blastocystis sp.

Terzioğlu¹,

Oguz²,

Bakkour³

et al. 2018

TMCD

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ÖZ Amaç: Çalışmada, ticari olarak geliştirilmiş bir dışkı yoğunlaştırma yöntemi olan Feconomics ® (Salubris, Türkiye) ile Feconomics ® 'in iki farklı modifikasyonunun, modifiye formol eter çöktürme (MFEÇ) yöntemiyle karşılaştırılarak Blastocystis spp.'nin mikroskobik tanısındaki etkinliğinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: Bu amaçla çeşitli gastrointestinal sistem yakınmaları ile gönderilen 750 hastaya ait dışkı örneği nativ-lugol yöntemi ile incelenmiş ve Blastocystis spp. saptanan 90 olgunun dışkı örneği çalışmaya dâhil edilmiştir. Dışkı örnekleri rutin uygulanan MFEÇ yöntemi, Feconomics ® ve Feconomics ® 'in iki farklı modifikasyonu ile yoğunlaştırılmıştır. Örnekler yoğunlaştırmadan önce ve sonra nativlugol ve trikrom boyama yöntemi ile incelenmiştir. Nativ-lugol inceleme ve trikrom boyama yöntemi, her alanda görülen protozoon sayısı ve protozoonların tipik morfolojik özellikleri açısından değerlendirilmiştir. Verilerin istatistiksel analizinde, IBM SPSS programı yardımıyla ki-kare testi kullanılmıştır ve p<0.05 değeri anlamlı olarak kabul edilmiştir. Bulgular: MFEÇ yöntemi ile yapılan yoğunlaştırma sonrası nativlugol inceleme, direkt nativ-lugol inceleme ile karşılaştırıldığında, her alanda görülen protozoon sayısı açısından istatistiksel fark anlamlı bulunmamıştır (nativ: p=0.104; lugol: p=0.168). Feconomics ® ve iki farklı modifikasyonu ile yapılan yoğunlaştırma sonrası nativlugol inceleme, direkt nativ-lugol inceleme ile karşılaştırıldığında ise her alanda görülen protozoon sayısı açısından istatistiksel fark anlamlı bulunmuştur (nativ: p=0.006, p=0.017, p=0.025; lugol: p<0.001, p<0.001, p=0.004). Feconomics ® ve iki farklı modifikasyonu sonrasında yapılan trikrom boyamada Blastocystis spp.'nin morfolojisinin bozulduğu ve tipik görünümlerinin kaybolduğu saptanmıştır. Sonuç: Feconomics ® 'in nativ-lugol inceleme için parazitoloji laboratuvarlarında hızlı ve etkili bir yoğunlaştırma yöntemi olarak kullanılabileceği sonucuna varılmıştır.

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Comparison of Conventional and Commercial Trichrome Staining Methods for Detecting Protozoan in Stool Samples

Cited by 3 publications

References 3 publications

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

Comparison of Microscopy, Boeck and Drbolav’s Stool Culture Medium and Bichro-latex Antibody Test with A Reference Elisa Antigen Test for E. histolytica Diagnosis in Calabar, Nigeria

Comparison of Various Methods in the Diagnosis of Entamoeba histolytica in Stool and Serum Specimens

A Comparison of a Modified Formalin-Ether Concentration Technique with a Commercial (Feconomics®) Method and Its Two Modifications in the Investigation of Blastocystis sp.

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