Comparison of conventional dried blood spots and volumetric absorptive microsampling for tacrolimus and mycophenolic acid determination

Paniagua-González, Lucía; Lendoiro, Elena; Otero-Antón, Esteban; López-Rivadulla, Manuel; de-Castro-Ríos, Ana; Cruz, Angelines

doi:10.1016/j.jpba.2021.114443

Cited by 25 publications

(45 citation statements)

References 27 publications

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“…The method employed here, volumetric absorptive microsampling, has been extensively evaluated for the obtention of dried blood specimens at a fixed volume for application to many bioanalytical purposes 13 . We propose its usefulness in the on-field collection of blood samples in situations such as the trail-running where access to specialized personnel or storage equipment might be limited.…”

Section: Discussionmentioning

confidence: 99%

“…The blood was left to dry in the open air until the next day and then stored frozen at -80 °C. The metabolites were extracted from the devices employing a mixture of water: acetonitrile (1:3, v:v), containing 13 C-labeled phenylalanine as internal standard, for one hour, keeping the samples at 4ºC. The extract was then centrifuged (13000 rpm, 15 minutes at room temperature), and the supernatant was stored at -20 °C to allow any proteins to precipitate.…”

Section: Sample Preparationmentioning

confidence: 99%

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Metabolomics reveals that fittest trail runners show a better adaptation of bioenergetic pathways

Puigarnau

Fernández

Òbis

et al. 2022

Journal of Science and Medicine in Sport

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Section: Discussionmentioning

confidence: 99%

Section: Sample Preparationmentioning

confidence: 99%

Metabolomics reveals that fittest trail runners show a better adaptation of bioenergetic pathways

Puigarnau

Fernández

Òbis

et al. 2022

Journal of Science and Medicine in Sport

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“…Disturbingly, a wide variability in the systematic DBS-to-plasma divergence is discernible between these studies, with Passing-Bablok slopes and mean concentration ratios ranging 0.51-0.78. 2,7,17,47 This translates to capillary DBS concentrations being generally 1.25-to 2-fold lower than venous plasma concentrations. Whereas individual haematocrit-based conversion seems the most sound approach from a theoretical perspective, the reported population haematocrits of 0.39-0.41 in these studies do to not fully explain the reported systematic biases.…”

Section: Discussionmentioning

confidence: 99%

“…Various validation studies have been conducted for liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays capable of quantifying tacrolimus, cyclosporine, everolimus, sirolimus and/or mycophenolic acid (MPA) in DBS, 2, VAMS, [34][35][36][37][38][39][40][41][42][43][44][45] or both. [46][47][48] Five of these assays allowed for the simultaneous quantification of 1 or more immunosuppressant and serum creatinine, 11,13,15,20,38,48 facilitating synchronised remote monitoring of kidney function and immunosuppressant exposure. However, the absence of MPA in these assays comprised an important limitation, as most kidney transplant recipients receive immunosuppressive therapy with tacrolimus, MPA and prednisolone.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, whereas the availability of such assays is important as a first step towards remote monitoring, subsequent clinical validation to demonstrate interchangeability with conventional venous sampling-based methods is crucial before considering these methods for routine clinical care. Whereas such clinical validation studies with DBS and/or VAMS samples have been performed for tacrolimus, either alone [34][35][36]42,46 or in combination with MPA, 2,7,47 sirolimus, 16 cyclosporine, 19,40 creatinine, 20,38,48 or creatinine and cyclosporine, 13 and for everolimus, either alone 44 or in combination with sirolimus, 4 this is not the case for MPA and creatinine, let alone tacrolimus in combination with MPA and creatinine.…”

Section: Introductionmentioning

confidence: 99%

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Volumetric microsampling for simultaneous remote immunosuppressant and kidney function monitoring in outpatient kidney transplant recipients

Zwart

Metscher

Boog

et al. 2022

Brit J Clinical Pharma

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Immunosuppressant and kidney function monitoring are crucial for kidney transplant recipient follow-up. Microsamples enable remote sampling and minimise patient burden as compared to conventional venous sampling at the clinic. We developed a liquid chromatography-tandem mass spectrometry assay to quantify tacrolimus, mycophenolic acid (MPA), creatinine and iohexol in dried blood spot (DBS), and volumetric absorptive microsample (VAMS) samples. Methods:The assay was successfully validated analytically for all analytes. Clinical validation was conducted by direct comparison of paired DBS, VAMS and venous reference samples from 25 kidney transplant recipients. Patients received iohexol 5-15 minutes before immunosuppressant intake and were sampled 0, 1, 2 and 3 hours thereafter, enabling tacrolimus and MPA area under the concentration-time curve (AUC) and creatinine-based and iohexol-based glomerular filtration rate (GFR) estimation. Method agreement was evaluated using Passing-Bablok regression, Bland-Altman analysis and the percentages of values within 15-30% of the reference (P 15 -P 30 ) with a P 20 acceptance threshold of 80%.Results: For DBS samples, method agreement was excellent for tacrolimus trough concentrations (n = 25, P 15 = 92.0%) and AUCs (n = 25; P 20 = 95.8%) and adequate for creatinine-based GFR trend monitoring (n = 25; P 20 = 80%). DBS-based MPA AUC assessment showed suboptimal agreement (n = 16; P 20 = 68.8%), but was considered acceptable given its P 30 of 100%. The assay performed inadequately for DBS-based iohexol GFR determination (n = 24; P 20 = 75%). The VAMS technique generally showed inferior performance, but can be considered for certain situations. Conclusion:The assay was successfully validated for tacrolimus, MPA and creatinine quantification in DBS samples, enabling simultaneous remote kidney function trendThe authors confirm that the Principal Investigator for this article is Dirk Jan Moes.

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Blood microsampling technologies: Innovations and applications in 2022

Thangavelu

Wouters

Kindt

et al. 2023

Analytical Science Advances

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With the development of highly sensitive bioanalytical techniques, the volume of samples necessary for accurate analysis has reduced. Microsampling, the process of obtaining small amounts of blood, has thus gained popularity as it offers minimal‐invasiveness, reduced logistical costs and biohazard risks while simultaneously showing increased sample stability and a potential for the decentralization of the approach and at‐home self‐sampling. Although the benefits of microsampling have been recognised, its adoption in clinical practice has been slow. Several microsampling technologies and devices are currently available and employed in research studies for various biomedical applications. This review provides an overview of the state‐of‐the‐art in microsampling technology with a focus on the latest developments and advancements in the field of microsampling. Research published in the year 2022, including studies (i) developing strategies for the quantitation of analytes in microsamples and (ii) bridging and comparing the interchangeability between matrices and choice of technology for a given application, is reviewed to assess the advantages, challenges and limitations of the current state of microsampling. Successful implementation of microsampling in routine clinical care requires continued efforts for standardization and harmonization. Microsampling has been shown to facilitate data‐rich studies and a patient‐centric approach to healthcare and is foreseen to play a central role in the future digital revolution of healthcare through continuous monitoring to improve the quality of life.

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Comparison of conventional dried blood spots and volumetric absorptive microsampling for tacrolimus and mycophenolic acid determination

Cited by 25 publications

References 27 publications

Metabolomics reveals that fittest trail runners show a better adaptation of bioenergetic pathways

Metabolomics reveals that fittest trail runners show a better adaptation of bioenergetic pathways

Volumetric microsampling for simultaneous remote immunosuppressant and kidney function monitoring in outpatient kidney transplant recipients

Blood microsampling technologies: Innovations and applications in 2022

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