Comparison of Conventional PCR and Multiplex Real-Time PCR Assay for Detection of &lt;i&gt;Staphylococcus aureus&lt;/i&gt; and &lt;i&gt;Bacillus cereus&lt;/i&gt;
in Ready-to-Eat Foods

Lee, Yu-Si; Kim, Seokhwan; Kim, Mi‐Gyeong; Kim, Soon Han

doi:10.13103/jfhs.2021.36.2.141

J. Fd Hyg. Safety

2021

DOI: 10.13103/jfhs.2021.36.2.141

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Comparison of Conventional PCR and Multiplex Real-Time PCR Assay for Detection of <i>Staphylococcus aureus</i> and <i>Bacillus cereus</i> in Ready-to-Eat Foods

Yu-Si Lee¹,

Seokhwan Kim²,

Mi‐Gyeong Kim³

et al.

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2023

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Cited by 2 publications

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Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

Byun¹,

Kim²,

Cho³

et al. 2023

Korean J. Food Preserv.

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The objective of this study is to compare and assess the effectiveness of real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), and the selective agar plate method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat (RTE) foods. In RTE foods, the detection performance of the three methods (RT-PCR [SureTectTM kit and PowerChekTM kit], LAMP [3M MDS], selective agar) were similar at 0-10, 10-50, 50-100, and 100- CFU/mL of Salmonella spp. and L. monocytogenes. We found that with RT-PCR, the Ct value of salad was significantly higher (p<0.05) than other RTE foods, indicating that fiber plays a critical role as an obstacle to the rapid detection of Salmonella spp. However, the Ct value displayed a mixed pattern according to the inoculation level of L. monocytogenes. The use of rapid detection kits and machines mostly depends on the user’s choice, with accuracy, ease of use, and economy being the primary considerations. As an RT-PCR kit, SureTectTM and PowerChekTM showed high accuracy in detecting Salmonella spp. and L. monocytogenes in RTE foods, showing that they can replace the existing RT-PCR kits available. Additionally, LAMP also showed excellent detection performance, suggesting that it has the potential to be used as a food safety management tool.

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Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

Byun¹,

Kim²,

Cho³

et al. 2023

Korean J. Food Preserv.

View full text Add to dashboard Cite

show abstract

Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

Byun¹,

Kim²,

Cho³

et al. 2023

Korean J. Food Preserv.

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show abstract

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Comparison of Conventional PCR and Multiplex Real-Time PCR Assay for Detection of <i>Staphylococcus aureus</i> and <i>Bacillus cereus</i> in Ready-to-Eat Foods

Cited by 2 publications

References 23 publications

Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

Comparison of SureTectTM with phenotypic and genotypic method for the detection of Salmonella spp. and Listeria monocytogenes in ready-to-eat foods

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